Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,389	G→A	A123A (GCC→GCT)	dhaL ←	dihydroxyacetone kinase, C‑terminal domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,389	0	G	A	100.0%	202.2 / NA	59	A123A (GCC→GCT)	dhaL	dihydroxyacetone kinase, C‑terminal domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

TGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGG  >  NC_000913/1249260‑1249514                                                                                                                                  |                                                                                                                              tgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCcg                                                                                                                      <  2:198564/139‑1 (MQ=255) tgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCcg                                                                                                                      >  1:698551/1‑139 (MQ=255) tgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCcg                                                                                                                      >  2:1296236/1‑139 (MQ=255)                            aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGa                                                                                                                   >  2:1475801/1‑115 (MQ=255)                            aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGa                                                                                                                   <  1:1475801/115‑1 (MQ=255)                              tttCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                         <  1:123474/138‑1 (MQ=255)                                        cACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGa                                                                               >  1:561515/1‑139 (MQ=255)                                                   agagaTTTTGCTCGCTAGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAACTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                    <  1:1179863/139‑1 (MQ=255)                                                     agaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                        >  1:1301963/1‑117 (MQ=255)                                                     agaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                        <  2:1301963/117‑1 (MQ=255)                                                         tttGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtgt                                                              <  2:698551/139‑1 (MQ=255)                                                                gCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCtt                                                       >  1:417161/1‑139 (MQ=255)                                                                 cTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCttt                                                      >  2:1432563/1‑139 (MQ=255)                                                                   ggACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    <  1:884012/139‑1 (MQ=255)                                                                            tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTc                                                             <  1:185583/121‑1 (MQ=255)                                                                            tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTc                                                             >  2:185583/1‑121 (MQ=255)                                                                            tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTgg                                           <  2:1061252/139‑1 (MQ=255)                                                                            tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTgg                                           >  2:991069/1‑139 (MQ=255)                                                                                   tCCACCACCGGCGCCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                          <  2:998955/69‑1 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                          >  1:998955/1‑69 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                         >  1:485731/1‑86 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                         <  2:485731/86‑1 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                         >  1:1165310/1‑86 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                         <  2:1165310/86‑1 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    >  2:792373/1‑123 (MQ=255)                                                                                   tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    <  1:792373/123‑1 (MQ=255)                                                                                    ccaccaCCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCtg                                   >  2:1310984/1‑139 (MQ=255)                                                                                     caccacCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcg                                                                                           >  1:47973/1‑82 (MQ=255)                                                                                     caccacCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcg                                                                                           <  2:47973/82‑1 (MQ=255)                                                                                          ccGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggc                             <  1:1310984/139‑1 (MQ=255)                                                                                             gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACa                                                                                    >  1:1259966/1‑81 (MQ=255)                                                                                             gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACa                                                                                    <  2:1259966/81‑1 (MQ=255)                                                                                             gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                            <  1:1155795/137‑1 (MQ=255)                                                                                             gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                            >  2:1155795/1‑137 (MQ=255)                                                                                             gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGcgcg                          >  1:1072008/1‑139 (MQ=255)                                                                                                    cacatcacacatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTG‑CAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAaaga                  <  1:408306/139‑1 (MQ=255)                                                                                                           cacatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTg                                                >  2:699360/1‑103 (MQ=255)                                                                                                           cacatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTg                                                <  1:699360/103‑1 (MQ=255)                                                                                                            acatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    >  1:1386916/1‑98 (MQ=255)                                                                                                            acatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    <  2:1386916/98‑1 (MQ=255)                                                                                                            acatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    >  1:228881/1‑98 (MQ=255)                                                                                                            acatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                    <  2:228881/98‑1 (MQ=255)                                                                                                             catGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCcgcg                                                                                                 <  2:3909/52‑1 (MQ=255)                                                                                                             catGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCcgcg                                                                                                 >  1:3909/1‑52 (MQ=255)                                                                                                               tGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCgcgc                                                                                                >  1:477844/1‑51 (MQ=255)                                                                                                               tGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCgcgc                                                                                                <  2:477844/51‑1 (MQ=255)                                                                                                                 gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                        <  1:1089032/57‑1 (MQ=255)                                                                                                                 gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                        >  2:1089032/1‑57 (MQ=255)                                                                                                                 gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGa                                                                                       >  2:828783/1‑58 (MQ=255)                                                                                                                 gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGa                                                                                       <  1:828783/58‑1 (MQ=255)                                                                                                                 gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACa      >  2:1121713/1‑139 (MQ=255)                                                                                                                  ttttATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAg     <  2:417161/139‑1 (MQ=255)                                                                                                                   tttATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGc    >  2:927158/1‑139 (MQ=255)                                                                                                                    ttATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCg   <  1:991069/139‑1 (MQ=255)                                                                                                                     tATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCgg  <  2:1072008/139‑1 (MQ=255)                                                                                                                          ccAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                    <  1:1155754/68‑1 (MQ=255)                                                                                                                          ccAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                    >  2:1155754/1‑68 (MQ=255)                                                                                                                            aGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                            >  2:977265/1‑106 (MQ=255)                                                                                                                            aGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                            <  1:977265/106‑1 (MQ=255)                                                                                                                                  |                                                                                                                              TGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGG  >  NC_000913/1249260‑1249514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑