Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,318,967 G→A D274D (GAC→GAT)  trpC ← indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,318,9670GA100.0% 201.9 / NA 59D274D (GAC→GAT) trpCindole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

GGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCGTCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGAA  >  NC_000913/1318835‑1319101
                                                                                                                                    |                                                                                                                                      
ggCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATaa                                                                                                                                  <  2:154405/139‑1 (MQ=255)
 gCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAg                                                                                                                                 <  1:1196384/139‑1 (MQ=255)
          tgtttGCGGAACACGCCCACATACTGCAACGGTGCCGCAGCCATCCCATCCTGCGCCTGTTCAACGTTGTAGCAAGGCCGCGATGTCCGAACATAGATCGACCGTCCGTAAATCACGCCCGCCTCATCCGCTGCTGTAg                                                                                                                        >  2:192988/4‑139 (MQ=255)
                     cacGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  2:247223/139‑1 (MQ=255)
                        gCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCAcgc                                                                                                          >  2:549320/1‑139 (MQ=255)
                        gCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCAcgc                                                                                                          >  1:1348872/1‑139 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCa                                                                                                      <  2:604381/133‑1 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCa                                                                                                      >  1:604381/1‑133 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  1:1122505/139‑1 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                >  2:621788/1‑139 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  1:621788/139‑1 (MQ=255)
                                  tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  1:1404619/139‑1 (MQ=255)
                                    cAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCTACTCGGTGATGTCGCAACAAAAATCAACTCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  1:923964/124‑1 (MQ=255)
                                    cAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACTCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             >  2:923964/1‑124 (MQ=255)
                                       cGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCttt                                                                                                                          >  2:326799/1‑108 (MQ=255)
                                       cGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCttt                                                                                                                          <  1:326799/108‑1 (MQ=255)
                                         gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAgg                                                                                                    >  1:1272270/1‑128 (MQ=255)
                                         gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAgg                                                                                                    <  2:1272270/128‑1 (MQ=255)
                                         gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcaca                                                                                               >  1:1269029/1‑133 (MQ=255)
                                         gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGCTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcaca                                                                                               <  2:1269029/133‑1 (MQ=255)
                                          tGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTa                                                                                        >  1:132254/1‑139 (MQ=255)
                                             cGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATtc                                                                                     <  2:1261056/139‑1 (MQ=255)
                                               cAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAg                                                                                                                                 >  1:826913/1‑93 (MQ=255)
                                               cAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAg                                                                                                                                 <  2:826913/93‑1 (MQ=255)
                                                 gCCATCACTTCCTGCGCCTGTTTAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCAc                                                                                 <  2:1050092/139‑1 (MQ=255)
                                                                 ccTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAA‑‑‑GCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCAc                                                              >  1:502554/1‑139 (MQ=255)
                                                                  cTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAA‑‑‑GCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCAcg                                                             <  2:502554/139‑1 (MQ=255)
                                                                   tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAgctg                                                                                                                              >  1:1402075/1‑76 (MQ=255)
                                                                   tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAgctg                                                                                                                              <  2:1402075/76‑1 (MQ=255)
                                                                   tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTa                                                                                                                         <  1:1121511/81‑1 (MQ=255)
                                                                   tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTa                                                                                                                         >  2:1121511/1‑81 (MQ=255)
                                                                   tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCa                                                               <  1:549320/139‑1 (MQ=255)
                                                                                          tGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  1:1314309/83‑1 (MQ=255)
                                                                                          tGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                >  2:1314309/1‑83 (MQ=255)
                                                                                               tCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       >  2:689417/1‑71 (MQ=255)
                                                                                               tCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       <  1:689417/71‑1 (MQ=255)
                                                                                               tCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCAt                                   <  2:132254/139‑1 (MQ=255)
                                                                                                  caacaaAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGCCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTg                                                     <  2:73584/118‑1 (MQ=255)
                                                                                                  caacaaAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGCCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTg                                                     >  1:73584/1‑118 (MQ=255)
                                                                                                    acaaAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACg                              <  2:170759/139‑1 (MQ=255)
                                                                                                             aaCCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCaa                     >  2:1392166/1‑139 (MQ=255)
                                                                                                             aaCCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCaa                     >  2:1376842/1‑139 (MQ=255)
                                                                                                                 cACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAg                 >  1:1345090/1‑139 (MQ=255)
                                                                                                                  aCCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  1:655681/46‑1 (MQ=255)
                                                                                                                  aCCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             >  2:655681/1‑46 (MQ=255)
                                                                                                                  aCCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCaa                     >  1:412811/1‑134 (MQ=255)
                                                                                                                  aCCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCaa                     <  2:412811/134‑1 (MQ=255)
                                                                                                                    cGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  2:796556/57‑1 (MQ=255)
                                                                                                                    cGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                >  1:796556/1‑57 (MQ=255)
                                                                                                                    cGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACt                                                                                           >  1:1110663/1‑62 (MQ=255)
                                                                                                                    cGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACt                                                                                           <  2:1110663/62‑1 (MQ=255)
                                                                                                                    cGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGaaa              >  1:1242167/1‑139 (MQ=255)
                                                                                                                        aaTCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCAcggcg                                                          >  2:778572/1‑91 (MQ=255)
                                                                                                                        aaTCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCAcggcg                                                          <  1:778572/91‑1 (MQ=255)
                                                                                                                         aTCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGt         >  2:965011/1‑139 (MQ=255)
                                                                                                                              gcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCg    >  1:1395715/1‑139 (MQ=255)
                                                                                                                              gcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCg    >  2:1440655/1‑139 (MQ=255)
                                                                                                                                ccGCATCATCAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGaa  <  1:965011/139‑1 (MQ=255)
                                                                                                                                ccGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGaa  <  1:192988/139‑1 (MQ=255)
                                                                                                                                ccGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGaa  <  1:1195521/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
GGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCGTCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGAA  >  NC_000913/1318835‑1319101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑