Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,458,573	T→C	C104R (TGT→CGT)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,458,573	0	T	C	100.0%	212.5 / NA	57	C104R (TGT→CGT)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/29);  total (28/29)

TAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTG  >  NC_000913/1458461‑1458702                                                                                                                 |                                                                                                                                  tAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCggcaggg                                                                                                         >  2:935991/1‑139 (MQ=255)  aaCGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCggcagggg                                                                                                        <  2:710589/139‑1 (MQ=255)      aTCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCggcaggggcagg                                                                                                    >  2:1414070/1‑139 (MQ=255)        cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCa                                                                                                                              <  2:677769/111‑1 (MQ=255)        cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCa                                                                                                                              >  1:677769/1‑111 (MQ=255)        cGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGgcg                                                                                                  >  2:645418/1‑139 (MQ=255)        cGTAACGTTGATCCCACAGGCCCCGCACCCGATTTAGGGATGTCAGTTGAACATTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGgcg                                                                                                  >  2:871380/1‑139 (MQ=255)          tAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCa                                                                                                >  1:1157529/1‑139 (MQ=255)              gTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAacac                                                                                            <  1:1089793/139‑1 (MQ=255)                  aTCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAAcactggc                                                                                        >  1:638620/1‑139 (MQ=255)                   tCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAAcactggcg                                                                                       >  1:10568/1‑138 (MQ=255)                   tCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAAcactggcg                                                                                       <  1:935991/139‑2 (MQ=255)                          ggCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCcgtg                                                                                                                                <  1:1327588/91‑1 (MQ=255)                          ggCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCcgtg                                                                                                                                >  2:1327588/1‑91 (MQ=255)                           gCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTgggggg                                                                               <  1:508160/139‑1 (MQ=255)                            ccccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGc                                                                              <  1:1386644/139‑1 (MQ=255)                            ccccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGc                                                                              <  1:330680/139‑1 (MQ=255)                             cccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCggcaggggca                                                                                                      <  2:624863/114‑1 (MQ=255)                             cccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCggcaggggca                                                                                                      >  1:624863/1‑114 (MQ=255)                              ccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            >  1:1404385/1‑139 (MQ=255)                              ccGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            <  1:645418/139‑1 (MQ=255)                                 cACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACAt                                                                         >  2:826428/1‑139 (MQ=255)                                                tGTCAGTTGAACGTTTCTATAACCAACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCg                                                          <  2:1345685/139‑1 (MQ=255)                                                 gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            <  1:1351460/120‑1 (MQ=255)                                                 gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            >  2:1351460/1‑120 (MQ=255)                                                  tCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGtt                                                        <  1:1414070/139‑1 (MQ=255)                                                              ttCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGgcg                                                                                                  >  2:635047/1‑85 (MQ=255)                                                              ttCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGgcg                                                                                                  <  1:635047/85‑1 (MQ=255)                                                                cTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTc                                          <  1:900183/139‑1 (MQ=255)                                                                 tataACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                         <  1:676189/139‑1 (MQ=255)                                                                        cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                           <  1:93725/98‑1 (MQ=255)                                                                        cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGAc                                                                           >  2:93725/1‑98 (MQ=255)                                                                         aCTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGttt                                 >  2:285001/1‑139 (MQ=255)                                                                          cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                >  1:661423/1‑139 (MQ=255)                                                                          cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                >  2:1450147/1‑139 (MQ=255)                                                                           tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAaca                                                                                             <  1:117115/77‑1 (MQ=255)                                                                           tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAaca                                                                                             >  2:117115/1‑77 (MQ=255)                                                                           tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                    >  2:996766/1‑134 (MQ=255)                                                                           tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                    <  1:996766/134‑1 (MQ=255)                                                                           tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAg                               <  2:638620/139‑1 (MQ=255)                                                                            ggTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGt                              <  2:1404385/139‑1 (MQ=255)                                                                               aCGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAg                           <  2:661423/139‑1 (MQ=255)                                                                                cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                    >  2:677489/1‑129 (MQ=255)                                                                                cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                    <  1:677489/129‑1 (MQ=255)                                                                                 gTCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                         >  1:672697/1‑139 (MQ=255)                                                                                  tCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                        <  2:10568/139‑1 (MQ=255)                                                                                  tCGCCTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                        >  2:942092/1‑139 (MQ=255)                                                                                     cgtGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTACCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                     >  2:904706/3‑139 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGGAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                <  2:1029397/128‑1 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            <  2:991643/84‑1 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGa                                                                            >  1:991643/1‑84 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                >  1:1029397/1‑128 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                          <  1:679594/134‑1 (MQ=255)                                                                                     ccTGGCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                          >  2:679594/1‑134 (MQ=255)                                                                                        ggCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAaca                                                                                             >  1:259038/1‑64 (MQ=255)                                                                                        ggCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAaca                                                                                             <  2:259038/64‑1 (MQ=255)                                                                                        ggCAAAACTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTa                  >  1:30206/1‑139 (MQ=255)                                                                                            aaaCTGCCAAAACCGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATAc              <  2:1444285/139‑1 (MQ=255)                                                                                                        ccGGTGATCCGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCGCTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTg  <  1:871380/139‑1 (MQ=255)                                                                                                                 |                                                                                                                                  TAACGATCGTAACGTTGATCCCACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCACTGGGGGGCGACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTG  >  NC_000913/1458461‑1458702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑