| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,610,596 | C→T | A29T (GCC→ACC) | uxaB ← | altronate oxidoreductase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,610,596 | 0 | C | T | 98.4% | 212.1 / NA | 63 | A29T (GCC→ACC) | uxaB | altronate oxidoreductase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (28/34); minor base A (1/0); total (29/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
AATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGA > NC_000913/1610465‑1610728 | aaTGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAgg > 1:933851/1‑139 (MQ=255) ggtggtGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAg < 2:274215/139‑1 (MQ=255) tgtACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTAcc > 1:147049/1‑139 (MQ=255) cAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCa > 1:1408229/1‑139 (MQ=255) cAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCa > 2:860955/1‑139 (MQ=255) gACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCAcc < 2:25892/139‑1 (MQ=255) gACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCAcc > 2:421834/1‑139 (MQ=255) aTCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACATCGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAAc > 2:725852/1‑139 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTAc < 2:271959/126‑1 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTAc > 1:271959/1‑126 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg > 2:1425332/1‑139 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg > 1:1381272/1‑139 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg > 1:317411/1‑139 (MQ=255) ccTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTg > 2:617733/1‑139 (MQ=255) gTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGa > 2:360920/1‑139 (MQ=255) gTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGa > 1:1276434/1‑139 (MQ=255) tGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt < 1:421834/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACATCGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 1:725852/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 2:360967/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 2:1408229/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 1:1496566/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 1:860955/139‑1 (MQ=255) cTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATAc < 1:1425332/139‑1 (MQ=255) cggcggGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGa < 1:44148/139‑1 (MQ=255) aaTGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGgtgcgc > 1:493891/1‑93 (MQ=255) aaTGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGgtgcgc < 2:493891/93‑1 (MQ=255) aaTGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACAGAATGATACGTTCTGGATACtgtg > 1:841466/1‑139 (MQ=255) gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgcg < 2:1381272/139‑1 (MQ=255) aCGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCaaa > 1:447290/1‑96 (MQ=255) aCGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCaaa < 2:447290/96‑1 (MQ=255) aacGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGAttt < 2:933851/139‑1 (MQ=255) cGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAgg > 2:654502/1‑71 (MQ=255) cGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAgg < 1:654502/71‑1 (MQ=255) cACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtt < 2:841466/139‑1 (MQ=255) cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt < 2:1115464/91‑1 (MQ=255) cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtttt > 1:727090/1‑139 (MQ=255) cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtttt > 2:530809/1‑139 (MQ=255) cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGtttt > 2:845085/1‑139 (MQ=255) cGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGAGCGCAGGAAGTTACCTTCACCAAACTGAATGAt > 1:1115464/1‑91 (MQ=255) ccAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTca < 2:594485/139‑1 (MQ=255) aaTTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAAt < 1:617733/139‑1 (MQ=255) aTCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTcc < 2:147049/139‑1 (MQ=255) ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt < 2:342290/75‑1 (MQ=255) ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt > 1:342290/1‑75 (MQ=255) tgtgCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGgtgcgc > 2:340323/1‑44 (MQ=255) tgtgCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGgtgcgc < 1:340323/44‑1 (MQ=255) tgtgCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCt > 1:853337/1‑139 (MQ=255) tgtgCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCt > 1:663238/1‑139 (MQ=255) tCATTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTa < 2:185953/139‑1 (MQ=255) aTTCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGt > 1:518419/1‑139 (MQ=255) ttCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAGACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTg < 2:954769/132‑1 (MQ=255) ttCAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTg > 1:954769/1‑132 (MQ=255) cAGGAGATCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCAt < 1:360920/139‑1 (MQ=255) agaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACaa < 2:518419/139‑1 (MQ=255) agaTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACaa < 1:808564/139‑1 (MQ=255) aTCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAAcc > 1:367378/1‑139 (MQ=255) tCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt < 2:435681/56‑1 (MQ=255) tCGATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGAt > 1:435681/1‑56 (MQ=255) gATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGt < 2:727090/139‑1 (MQ=255) gATTTGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGt < 2:809603/139‑1 (MQ=255) tGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGG‑‑ACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTa < 2:1142063/119‑1 (MQ=255) tGCCAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGG‑‑ACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTa > 1:1142063/1‑119 (MQ=255) ccAGTCAACAAAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAAt < 1:845085/139‑1 (MQ=255) aacaaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGt < 2:1500048/46‑1 (MQ=255) aacaaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGt > 1:1500048/1‑46 (MQ=255) acaaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACg < 2:663238/139‑1 (MQ=255) caaAGGTGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGa < 2:1276434/139‑1 (MQ=255) | AATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGA > NC_000913/1610465‑1610728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |