Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,677,937	T→C	intergenic (‑34/‑490)	pntA ← / → ydgH	pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,677,937	0	T	C	100.0%	192.2 / NA	52	intergenic (‑34/‑490)	pntA/ydgH	pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

CGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAT  >  NC_000913/1677807‑1678061                                                                                                                                   |                                                                                                                             cGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCtgt                                                                                                                      >  1:358737/1‑139 (MQ=255)   gTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTgtgt                                                                                                                    >  1:1010531/1‑139 (MQ=255)           cAGTTTCAGCAGCTGTTCCACTG‑TTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTc                                                                                                                   >  2:162901/1‑131 (MQ=255)           cAGTTTCAGCAGCTGTTCCACTG‑TTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTc                                                                                                                   <  1:162901/131‑1 (MQ=255)                  agcagcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     <  2:1503065/139‑1 (MQ=255)                  agcagcTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     >  1:670504/1‑139 (MQ=255)                        tGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTc                                                                                               >  2:516339/1‑139 (MQ=255)                        tGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTc                                                                                               >  1:902496/1‑139 (MQ=255)                              aCTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTAc                                                                                         <  1:156609/139‑1 (MQ=255)                              aCTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTAc                                                                                         <  1:516339/139‑1 (MQ=255)                                   ttttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCt                                                                                    <  2:670504/139‑1 (MQ=255)                                    tttGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTa                                                                                   >  2:1163075/1‑139 (MQ=255)                                                 cAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTAAGAGCTAAATGTTACTCCGt                                                                      <  2:358737/139‑1 (MQ=255)                                                          tttCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAaaataaa                                                             <  2:1010531/139‑1 (MQ=255)                                                                  gTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCT‑‑ATGTTACTCCGTTAAAATAAATTAGTAACaa                                                   <  1:814081/139‑1 (MQ=255)                                                                    tAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaa                                                   >  1:618124/1‑139 (MQ=255)                                                                     aaCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                  >  2:681228/1‑139 (MQ=255)                                                                     aaCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                  <  2:902496/139‑1 (MQ=255)                                                                      aCCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAAc                                                 <  2:618124/139‑1 (MQ=255)                                                                       aggTTCTCTTGGTATGCCAATTCGGATTATATTCCCTGCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTAATGTACGAGCTTAATGTTACTCCGTTAAAATAAATTAGTAACAAACg                                                <  2:1130555/137‑1 (MQ=255)                                                                          ttCTCTTGGTACGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                  >  1:358911/1‑134 (MQ=255)                                                                          ttCTCTTGGTACGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACaaa                                                  <  2:358911/134‑1 (MQ=255)                                                                            ctctTGGTATGCCAATTCGCATGTTATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCta                                           >  2:269420/1‑139 (MQ=255)                                                                               ttGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTATATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATaa                                        >  1:184537/1‑139 (MQ=255)                                                                                 ggTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACg                                      >  1:1276178/1‑139 (MQ=255)                                                                                    aTGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAATCTGCTCATGTACGAGCTTAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGtct                                   >  1:1130555/1‑137 (MQ=255)                                                                                         aaTTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     <  1:30525/68‑1 (MQ=255)                                                                                         aaTTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     >  2:30525/1‑68 (MQ=255)                                                                                           ttCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAAt                            >  2:794554/1‑139 (MQ=255)                                                                                            tCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     <  2:643767/65‑1 (MQ=255)                                                                                            tCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     >  1:643767/1‑65 (MQ=255)                                                                                              gCATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTAt                         <  1:794554/139‑1 (MQ=255)                                                                                               cATGATATTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATg                        >  1:328031/1‑139 (MQ=255)                                                                                                   atatTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     <  1:1089029/58‑1 (MQ=255)                                                                                                   atatTCCCTTCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAg                                                                                                     >  2:1089029/1‑58 (MQ=255)                                                                                                            tCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCtata                                         >  1:376477/1‑109 (MQ=255)                                                                                                            tCCATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCtata                                         <  2:376477/109‑1 (MQ=255)                                                                                                             ccATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAgcg          >  2:349335/1‑139 (MQ=255)                                                                                                              cATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCTTGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTTAAAATTATGCCTGTGATCTAgcgc         >  1:841355/1‑139 (MQ=255)                                                                                                              cATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCt                                                                                    >  2:887742/1‑64 (MQ=255)                                                                                                              cATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCt                                                                                    <  1:887742/64‑1 (MQ=255)                                                                                                              cATCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAgcgc         >  1:22401/1‑139 (MQ=255)                                                                                                                tCGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCa       >  2:1411125/1‑139 (MQ=255)                                                                                                                 cGGTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTg                                 <  1:885655/112‑1 (MQ=255)                                                                                                                 cGGTTTTATTGATGATGGCTGGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTg                                 >  2:885655/1‑112 (MQ=255)                                                                                                                   gTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa    >  2:1033368/1‑139 (MQ=255)                                                                                                                   gTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa    >  2:1054622/1‑139 (MQ=255)                                                                                                                   gTTTTATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCaaaa    <  1:269420/139‑1 (MQ=255)                                                                                                                     tttATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCTTGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAt  <  2:841355/139‑1 (MQ=255)                                                                                                                     tttATTGATGATGGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAt  <  2:184537/139‑1 (MQ=255)                                                                                                                              gatgGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAAc                                                     >  1:685909/1‑79 (MQ=255)                                                                                                                              gatgGCTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAAc                                                     <  2:685909/79‑1 (MQ=255)                                                                                                                                   |                                                                                                                             CGGTAAAACCCAGTTTCAGCAGCTGTTCCACTGTTTTTGGCGTTGCTGCAACACGGGTTTCATTGGTTAACCGTTCTCTTGGTATGCCAATTCGCATGATATTCCCTTCCATCGGTTTTATTGATGATGGTTTGCCTGTGTCAGGAGCCACACAAGCTGCTCATGTACGAGCTAAATGTTACTCCGTTAAAATAAATTAGTAACAAACGCCCTATAACGTACTGAAAATTATGCCTGTGATCTAGCGCCAAAAAT  >  NC_000913/1677807‑1678061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑