Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,343	A→G	Y167C (TAC→TGC)	purR →	transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,343	0	A	G	100.0%	234.4 / NA	63	Y167C (TAC→TGC)	purR	transcriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/34);  total (29/34)

GTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATC  >  NC_000913/1738211‑1738470                                                                                                                                     |                                                                                                                                gtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           <  1:402535/139‑1 (MQ=255) gtTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           <  2:1273387/139‑1 (MQ=255)    cTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCgg                                                                                                                        >  2:68599/1‑139 (MQ=255)     tGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCggg                                                                                                                       <  2:1129088/139‑1 (MQ=255)           cccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                 <  1:497952/139‑1 (MQ=255)            ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     <  2:555389/134‑1 (MQ=255)            ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     <  2:591904/134‑1 (MQ=255)            ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     >  1:555389/1‑134 (MQ=255)            ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     >  1:591904/1‑134 (MQ=255)            ccAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATc                                                                                                                >  2:434352/1‑139 (MQ=255)               gagCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGa                                                                                                             <  1:283262/139‑1 (MQ=255)                 gCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGAtt                                                                                                           >  1:515474/1‑139 (MQ=255)                 gCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGAtt                                                                                                           >  1:613619/1‑139 (MQ=255)                           gCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTc                                                                                                 <  1:145090/139‑1 (MQ=255)                               tGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACcg                                                                                             <  1:68599/139‑1 (MQ=255)                                         gTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGc                                                                                   >  2:56497/1‑139 (MQ=255)                                          tATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCg                                                                                  >  1:1388328/1‑139 (MQ=255)                                            tCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTc                                                                                <  2:1230747/139‑1 (MQ=255)                                                  tatCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           <  1:858739/90‑1 (MQ=255)                                                  tatCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           >  2:858739/1‑90 (MQ=255)                                                         aTGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGc                                                                   <  2:554361/139‑1 (MQ=255)                                                          tggtggTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCt                                                                  >  2:1330104/1‑139 (MQ=255)                                                              ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           <  1:719304/78‑1 (MQ=255)                                                              ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           >  2:719304/1‑78 (MQ=255)                                                              ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                 <  1:1325984/88‑1 (MQ=255)                                                              ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                 >  2:1325984/1‑88 (MQ=255)                                                              ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                              >  2:1145427/1‑139 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     >  1:1346800/1‑83 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                     <  2:1346800/83‑1 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAAcg                                                                                                      <  2:568908/98‑1 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAAcg                                                                                                      >  1:568908/1‑98 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAAc                                                             >  2:631293/1‑139 (MQ=255)                                                               gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAAc                                                             >  1:478838/1‑139 (MQ=255)                                                                   tGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAAt                                                                                       >  2:127982/1‑109 (MQ=255)                                                                   tGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAAt                                                                                       <  1:127982/109‑1 (MQ=255)                                                                         ggggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                 <  1:1509147/77‑1 (MQ=255)                                                                         ggggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                 >  2:1509147/1‑77 (MQ=255)                                                                          gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGaaa                                                                                        >  1:570182/1‑101 (MQ=255)                                                                          gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGaaa                                                                                        <  2:570182/101‑1 (MQ=255)                                                                               aaGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGc                                                                   <  2:487975/117‑1 (MQ=255)                                                                               aaGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGc                                                                   >  1:487975/1‑117 (MQ=255)                                                                                       gCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTg                                     <  1:631293/139‑1 (MQ=255)                                                                                          gACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAacac                                                      <  1:1492829/119‑1 (MQ=255)                                                                                          gACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAacac                                                      >  2:1492829/1‑119 (MQ=255)                                                                                           aCTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCgg                                 >  2:543122/1‑139 (MQ=255)                                                                                                  cGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           <  1:476308/42‑1 (MQ=255)                                                                                                  cGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGc                                                                                                                           >  2:476308/1‑42 (MQ=255)                                                                                                  cGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                          <  1:1330104/139‑1 (MQ=255)                                                                                                   gATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGa                         <  1:7602/139‑1 (MQ=255)                                                                                                   gATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGACCGCCTTGCCGGTTTTATGa                         <  1:1145427/139‑1 (MQ=255)                                                                                                    aTGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGaa                        <  2:669494/139‑1 (MQ=255)                                                                                                    aTGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGaa                        <  1:543122/139‑1 (MQ=255)                                                                                                     tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg                       <  2:394160/139‑1 (MQ=255)                                                                                                            aTTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATgg                >  1:555706/1‑139 (MQ=255)                                                                                                               gATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaag             <  2:515474/139‑1 (MQ=255)                                                                                                                    cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTc                                                                                                 >  1:365212/1‑50 (MQ=255)                                                                                                                    cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTc                                                                                                 <  2:365212/50‑1 (MQ=255)                                                                                                                      cgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTc                                                                                                 >  1:122811/1‑48 (MQ=255)                                                                                                                      cgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTc                                                                                                 <  2:122811/48‑1 (MQ=255)                                                                                                                      cgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCgat      >  2:1463662/1‑139 (MQ=255)                                                                                                                          cGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATc  <  2:613619/139‑1 (MQ=255)                                                                                                                                  gcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTg                                     <  1:1263853/96‑1 (MQ=255)                                                                                                                                  gcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                          >  1:590900/1‑107 (MQ=255)                                                                                                                                     |                                                                                                                                GTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATC  >  NC_000913/1738211‑1738470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑