Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,818,420 A→G R27R (CGT→CGC)  chbF ← phospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,818,4200AG100.0% 245.5 / NA 67R27R (CGT→CGC) chbFphospho‑chitobiase, general 6‑phospho‑beta‑glucosidase activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/35);  total (32/35)

AGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCG  >  NC_000913/1818298‑1818551
                                                                                                                          |                                                                                                                                   
aGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCtt                                                                                                                     >  1:45459/1‑139 (MQ=255)
  cTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTcc                                                                                                                   >  2:717359/1‑139 (MQ=255)
     cATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGt                                                                                                                >  1:1268677/1‑139 (MQ=255)
       tCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTtaa                                                                                                                              <  1:394482/123‑1 (MQ=255)
       tCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTtaa                                                                                                                              >  2:394482/1‑123 (MQ=255)
                    gTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAg                                                                                                 <  2:45459/139‑1 (MQ=255)
                       atcaatcaTCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgctg                                                                                              >  1:1158782/1‑139 (MQ=255)
                          aatcaTCCGTTGGCTGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCAGCATCCATAATTCGCTGACCGGCAAGTCGTGATAGTGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgctgctt                                                                                           <  1:925127/139‑2 (MQ=255)
                              atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGt                                                                                                                <  1:752724/114‑1 (MQ=255)
                              atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGt                                                                                                                >  2:752724/1‑114 (MQ=255)
                              atcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGc                                                                                       <  1:1450401/139‑1 (MQ=255)
                               tcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           <  2:637097/118‑1 (MQ=255)
                               tcCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           >  1:637097/1‑118 (MQ=255)
                                 cGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgctgc                                                                                             >  2:353520/1‑130 (MQ=255)
                                 cGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgctgc                                                                                             <  1:353520/130‑1 (MQ=255)
                                 cGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGccac                                                                                    <  1:121706/139‑1 (MQ=255)
                                          agaTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTga                                                                           >  1:712451/1‑139 (MQ=255)
                                            aTCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTgacg                                                                         >  1:1035353/1‑139 (MQ=255)
                                             tCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCg                                                                                                                                   <  2:1047940/80‑1 (MQ=255)
                                             tCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCg                                                                                                                                   >  1:1047940/1‑80 (MQ=255)
                                             tCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGAT‑GCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgct                                                                                               <  2:133827/115‑1 (MQ=255)
                                             tCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGAT‑GCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAgct                                                                                               >  1:133827/1‑115 (MQ=255)
                                                      atatCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTgac                                                                          >  2:915442/1‑128 (MQ=255)
                                                      atatCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTgac                                                                          <  1:915442/128‑1 (MQ=255)
                                                       tatCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATCGTGACGACTTTTAAttt                                                              >  1:69063/1‑139 (MQ=255)
                                                        atCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTc                                                             <  1:717359/139‑1 (MQ=255)
                                                            aGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGc                                                         >  1:796904/1‑139 (MQ=255)
                                                              tttCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           >  1:30557/1‑87 (MQ=255)
                                                              tttCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           <  2:30557/87‑1 (MQ=255)
                                                                       accaccTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTtct                                              >  1:442921/1‑139 (MQ=255)
                                                                       accaccTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTCAATTTCTGGCTCATAATTtct                                              <  1:685218/139‑1 (MQ=255)
                                                                       accaccTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCACCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTtct                                              <  1:694948/139‑1 (MQ=255)
                                                                         caccTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTcc                                            <  2:1035353/139‑1 (MQ=255)
                                                                            cTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCtt                                         <  2:1158782/139‑1 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCt                                                                                                                      >  2:1312914/1‑60 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCt                                                                                                                      >  2:97352/1‑60 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCt                                                                                                                      <  1:97352/60‑1 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCt                                                                                                                      <  1:1312914/60‑1 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           <  1:1461758/71‑1 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTc                                                                                                           >  2:1461758/1‑71 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTgg                                                          <  2:580073/120‑1 (MQ=255)
                                                                              tCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTgg                                                          >  1:580073/1‑120 (MQ=255)
                                                                                 acgtCCACAAGCCATAATTAGCGGCCCGGCAATTCGTGATAGCGCTTATTAAATCCATCCAGTAACTCCGGGGCGTAGCTCGTCCCGCCACCAATACTGACGACTTTTAATTTCTGGCTCAGAATTTCTCCCTTCAGTa                                    <  2:1112169/136‑1 (MQ=255)
                                                                                 aCATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTa                                    <  1:952204/139‑1 (MQ=255)
                                                                                 aCATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTa                                    <  2:69063/139‑1 (MQ=255)
                                                                                 aCATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTa                                    <  2:1268677/139‑1 (MQ=255)
                                                                                   aTCCGCCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATc                                                                                                                        <  1:195324/53‑1 (MQ=255)
                                                                                   aTCCCCCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATc                                                                                                                        >  2:195324/1‑53 (MQ=255)
                                                                                    tCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAg                                 <  2:712451/139‑1 (MQ=255)
                                                                                     ccaccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGa                                >  1:1361068/1‑139 (MQ=255)
                                                                                     ccaccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGa                                >  2:1513555/1‑139 (MQ=255)
                                                                                      caccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTccc                                                                                         >  2:921974/1‑81 (MQ=255)
                                                                                      caccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTccc                                                                                         <  1:921974/81‑1 (MQ=255)
                                                                                      caccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGcc                                                                                      >  2:2878/1‑84 (MQ=255)
                                                                                      caccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGcc                                                                                      <  1:2878/84‑1 (MQ=255)
                                                                                      caccaGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGaa                               >  1:1242630/1‑139 (MQ=255)
                                                                                         caGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTcc                                                                                                                   >  2:1455885/1‑52 (MQ=255)
                                                                                         caGCCATAATTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTcc                                                                                                                   <  1:1455885/52‑1 (MQ=255)
                                                                                                aaTTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGt                                                                                                                <  2:450234/48‑1 (MQ=255)
                                                                                                aaTTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGt                                                                                                                >  1:450234/1‑48 (MQ=255)
                                                                                                aaTTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCt                                                            <  2:93965/100‑1 (MQ=255)
                                                                                                aaTTCGCTGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCt                                                            >  1:93965/1‑100 (MQ=255)
                                                                                                       tGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTtct                                              >  2:831744/1‑107 (MQ=255)
                                                                                                       tGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTtct                                              <  1:831744/107‑1 (MQ=255)
                                                                                                       tGACCGGCAATTCGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCata              >  1:1143179/1‑139 (MQ=255)
                                                                                                                   cGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCg  <  1:1513555/139‑1 (MQ=255)
                                                                                                                   cGTGATAGCGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCg  <  2:1361068/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                   
AGCTTCATCGGGACGCCAGCGTTATCAATCATCCGTTGGCAGAGATCGAAAATAATATCCAGTTTCGGTTTACCACCTTCGACATCCACCAGCCATAATTCGCTGACCGGCAATTCGTGATAACGCTTAATAAATCCTTCCAGTAACTCCGGGGTATAGCTGCTCCCGCCACCAATAGTGACGACTTTTAATTTCTGGCTCATAATTTCTCCCTTCAGTACAGAATACTGATATCTGGCATATCTGCCCCCCCG  >  NC_000913/1818298‑1818551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑