| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,893,102 | G→A | A45V (GCA→GTA) | yoaA ← | putative ATP‑dependent helicase, DinG family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,893,102 | 0 | G | A | 94.1% | 110.1 / ‑0.4 | 34 | coding (134/1911 nt) | yoaA | putative ATP‑dependent helicase, DinG family |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/18); minor base . (1/1); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACA > NC_000913/1892971‑1893236 | ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccac > 1:517488/1‑139 (MQ=255) gaCTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccaccacc < 2:373357/139‑1 (MQ=255) gcgGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCtttt > 1:10886/1‑139 (MQ=255) ggCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTc < 2:474054/139‑1 (MQ=255) agagCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGc < 1:1192747/139‑1 (MQ=255) gATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTCCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGt < 2:942548/139‑1 (MQ=255) ccTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGAc > 2:219791/1‑139 (MQ=255) gCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTa < 1:19235/123‑1 (MQ=255) gCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTa > 2:19235/1‑123 (MQ=255) cAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCt < 1:461661/139‑1 (MQ=255) gCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCg > 1:860272/1‑139 (MQ=255) aGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTg < 1:728762/139‑1 (MQ=255) gaTAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTgg > 1:65892/1‑91 (MQ=255) gaTAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTgg < 2:65892/91‑1 (MQ=255) tttttCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCgg > 1:606629/1‑74 (MQ=255) tttttCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCgg < 2:606629/74‑1 (MQ=255) cGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCt < 2:749879/76‑1 (MQ=255) cGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCt > 1:749879/1‑76 (MQ=255) gcagcgcagGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccaccacc > 1:647750/1‑59 (MQ=255) gcagcgcagGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccaccacc < 2:647750/59‑1 (MQ=255) cGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTc < 1:219791/139‑1 (MQ=255) cGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTCTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTc < 1:955585/139‑1 (MQ=255) ggTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGt > 1:1028591/1‑139 (MQ=255) gTTTTGCCCGTACCGGTTCCT‑CTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGc > 1:704662/1‑139 (MQ=255) tGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGa < 1:85237/60‑1 (MQ=255) tGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGa > 2:85237/1‑60 (MQ=255) tACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATc < 2:572642/68‑1 (MQ=255) tACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATc > 1:572642/1‑68 (MQ=255) tACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATcgtc > 1:1215493/1‑139 (MQ=255) ccGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATcgtccg < 2:860272/139‑1 (MQ=255) cGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATcgtccgt < 2:10886/139‑1 (MQ=255) ggTTCCT‑CTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTca < 2:704662/139‑1 (MQ=255) gTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTca > 2:201999/1‑139 (MQ=255) tCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTcaca < 1:859078/139‑1 (MQ=255) | TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAATCGTCCGTCACA > NC_000913/1892971‑1893236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |