Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,127,360	T→C	T319A (ACG→GCG)	gmd ←	GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,127,360	0	T	C	100.0%	186.0 / NA	50	T319A (ACG→GCG)	gmd	GDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/22);  total (28/22)

CCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGCCCTTCTCTTCAACGCC  >  NC_000913/2127224‑2127477                                                                                                                                         |                                                                                                                      ccGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGC‑TTGGTCGTGTCGCCGAGCAGCGCTTc                                                                                                                    >  2:586897/1‑139 (MQ=255)    tGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCaa                                                                                                                  >  1:599967/1‑139 (MQ=255)      agaTTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACt                                                                                                                >  2:100791/1‑139 (MQ=255)                     gtgtTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAg                                                                                                 <  1:432917/139‑1 (MQ=255)                           tttCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAAcgc                                                                                           >  1:781036/1‑139 (MQ=255)                                ccgcTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTc                                                                                      >  2:553153/1‑139 (MQ=255)                                ccgcTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTc                                                                                      >  2:435057/1‑139 (MQ=255)                                  gcTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTCTCGTTCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                                    >  2:1082002/1‑139 (MQ=255)                                   cTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGTGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAAc                                                                                   <  1:1117039/139‑1 (MQ=255)                                      cGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGc                                                                                <  1:553153/139‑1 (MQ=255)                                                     ccaTTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCAcc                                                                 >  2:826539/1‑139 (MQ=255)                                                               cacCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACg                                                       <  2:260481/139‑1 (MQ=255)                                                                  cATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGccc                                                    >  2:8933/1‑139 (MQ=255)                                                                  cATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGccc                                                    >  2:495510/1‑139 (MQ=255)                                                                   aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCa                                                                                     >  2:532064/1‑105 (MQ=255)                                                                   aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCa                                                                                     <  1:532064/105‑1 (MQ=255)                                                                   aTCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCg                                                   >  2:484156/1‑139 (MQ=255)                                                                          tGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGc                                                                                                           <  1:1321189/76‑1 (MQ=255)                                                                          tGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGc                                                                                                           >  2:1321189/1‑76 (MQ=255)                                                                          tGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTc                                            <  1:100791/139‑1 (MQ=255)                                                                              ggTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGc                                        >  1:60302/1‑139 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCcggac                                                                                                      <  1:136910/73‑1 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCcggac                                                                                                      >  2:136910/1‑73 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCgg                                    >  1:805518/1‑139 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCgg                                    >  1:1121344/1‑139 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCgg                                    >  2:1176157/1‑139 (MQ=255)                                                                                  aTTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCgg                                    >  1:1179418/1‑139 (MQ=255)                                                                                       cGGTTTCCAGCCCAGTTTCTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACg                               <  1:1082002/139‑1 (MQ=255)                                                                                           ttCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGaaa                           <  2:60302/139‑1 (MQ=255)                                                                                           ttCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGaaa                           <  1:1174036/139‑1 (MQ=255)                                                                                            tCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCcgga                                                                                                       <  1:1064040/62‑1 (MQ=255)                                                                                            tCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCcgga                                                                                                       >  2:1064040/1‑62 (MQ=255)                                                                                                     gTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGcc                 <  2:599967/139‑1 (MQ=255)                                                                                                        tttCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGCCCtt              <  2:1179418/139‑1 (MQ=255)                                                                                                           cGTGCGCTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGCCCTtctc           >  1:1096374/1‑139 (MQ=255)                                                                                                               cgcTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCAccc                                                                >  1:831139/1‑82 (MQ=255)                                                                                                               cgcTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCAccc                                                                <  2:831139/82‑1 (MQ=255)                                                                                                                gcTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                                    >  2:1031876/1‑61 (MQ=255)                                                                                                                gcTTTGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                                    <  1:1031876/61‑1 (MQ=255)                                                                                                                    tGGTCGGGTCGCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGCCCTTCTCTTCAACGcc  <  1:1023939/139‑1 (MQ=255)                                                                                                                      gtcgggtcgCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTaa                                                                                              <  1:599660/45‑1 (MQ=255)                                                                                                                      gtcgggtcgCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTaa                                                                                              >  2:599660/1‑45 (MQ=255)                                                                                                                              gCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGAc                                <  2:464075/99‑1 (MQ=255)                                                                                                                              gCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGAc                                >  1:464075/1‑99 (MQ=255)                                                                                                                              gCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGaaa                           >  1:265847/1‑104 (MQ=255)                                                                                                                              gCCGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGaaa                           <  2:265847/104‑1 (MQ=255)                                                                                                                               ccGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCgg                                                                                         >  1:1018012/1‑41 (MQ=255)                                                                                                                               ccGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCgg                                                                                         <  2:1018012/41‑1 (MQ=255)                                                                                                                                cGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                                    >  2:1128732/1‑45 (MQ=255)                                                                                                                                cGAGCAGCGCTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCaa                                                                                    <  1:1128732/45‑1 (MQ=255)                                                                                                                                         |                                                                                                                      CCGTGAGATTTCAGCAGAGAGTGTTTTTTCGCCGCTTCGAGGTCATTAGCCACCATTTCAGACACCATCTCTCTGAGGGTGATTTCCGGTTTCCAGCCCAGTTTTTCGTGCGCTTTGGTCGGGTCGCCGAGCAGCGTTTCAACTTCAGCCGGACGGAAGTAACGCGGGTCAACAGCGATAATCACATCACCCGGTTTAACGCCCGGCGCGTCATGCCCGGTGACGGAAACCACAATGCCCTTCTCTTCAACGCC  >  NC_000913/2127224‑2127477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑