Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,248,780 C→T R280Q (CGG→CAG)  yeiE ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,248,7800CT100.0% 309.2 / NA 90R280Q (CGG→CAG) yeiEputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/46);  total (44/46)

AAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG  >  NC_000913/2248649‑2248911
                                                                                                                                   |                                                                                                                                   
aaGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcgc                                                                                                                              >  2:457164/1‑139 (MQ=255)
      ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaa                                                                                                                       >  1:1201451/1‑139 (MQ=255)
      ccAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaa                                                                                                                       >  1:344093/1‑139 (MQ=255)
       cAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      <  2:1201451/139‑1 (MQ=255)
                ttCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGtttt                                                                                                              >  1:343510/1‑139 (MQ=255)
                   tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                                  >  2:1343713/1‑116 (MQ=255)
                   tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                                  <  1:1343713/116‑1 (MQ=255)
                   tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           <  1:962809/139‑1 (MQ=255)
                   tGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           <  1:1475058/139‑1 (MQ=255)
                    ggCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGcc                                                                                                          >  1:539866/1‑139 (MQ=255)
                     gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         >  2:1032942/1‑139 (MQ=255)
                     gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         <  1:457164/139‑1 (MQ=255)
                        aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  2:128489/1‑139 (MQ=255)
                        aaTGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAACATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  2:1067717/1‑139 (MQ=255)
                          tgcatgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatgat                                                                                                    >  2:1105474/1‑139 (MQ=255)
                          tgcatgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  1:675642/1‑137 (MQ=255)
                          tgcatgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      <  2:675642/137‑1 (MQ=255)
                          tccatgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTCTAGCGCGTTGGAAAGGTGTTTTTGCCgatgat                                                                                                    <  2:1015974/137‑1 (MQ=255)
                              tgcTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGtat                                                                                                <  2:343510/139‑1 (MQ=255)
                                   aaGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc                                                                                           >  1:807090/1‑139 (MQ=255)
                                     ggCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGGTGATGTATACGCcac                                                                                         >  1:1010611/1‑139 (MQ=255)
                                      gcgcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                        <  2:539866/139‑1 (MQ=255)
                                                                       tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           <  1:622237/87‑1 (MQ=255)
                                                                       tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           >  2:622237/1‑87 (MQ=255)
                                                                       tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcac                                                                                         <  2:1419237/105‑1 (MQ=255)
                                                                       tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcac                                                                                         >  1:1419237/1‑105 (MQ=255)
                                                                        gTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCTGATGGACCGCAAc                                                      <  2:1010611/139‑1 (MQ=255)
                                                                        gTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc                                                      <  1:631905/139‑1 (MQ=255)
                                                                         ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                     >  2:512190/1‑107 (MQ=255)
                                                                         ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACg                                                                                     <  1:512190/107‑1 (MQ=255)
                                                                           cTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      >  2:687680/1‑72 (MQ=255)
                                                                           cTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      <  1:687680/72‑1 (MQ=255)
                                                                              gTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGTAGAGGGACCGCAACTTCACt                                                >  1:1173543/1‑139 (MQ=255)
                                                                                  aGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAt                                            >  1:992533/1‑139 (MQ=255)
                                                                                   gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGCAAGGTGTTTTTGCCg                                                                                                         >  1:827797/1‑77 (MQ=255)
                                                                                   gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         <  2:827797/77‑1 (MQ=255)
                                                                                   gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                         <  1:1507236/109‑1 (MQ=255)
                                                                                   gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                         >  2:1507236/1‑109 (MQ=255)
                                                                                   gCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtg                                           >  1:584895/1‑139 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGGGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc                                                      <  2:1221282/127‑1 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGGGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc                                                      >  1:1221282/1‑127 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCAc                                                 <  2:1410792/132‑1 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCAc                                                 >  1:1410792/1‑132 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtgt                                          >  2:820267/1‑139 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtgt                                          >  1:1024675/1‑139 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtgt                                          >  1:408233/1‑139 (MQ=255)
                                                                                    cAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAAtgt                                          >  1:1183073/1‑139 (MQ=255)
                                                                                      aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAATGGTGTTTTTGc                                                                                                           <  2:874471/72‑1 (MQ=255)
                                                                                      aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           >  1:874471/1‑72 (MQ=255)
                                                                                      aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCaga                                                                 >  1:1043822/1‑114 (MQ=255)
                                                                                      aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCaga                                                                 <  2:1043822/114‑1 (MQ=255)
                                                                                        ttAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcct                                      <  2:1024675/139‑1 (MQ=255)
                                                                                           aCGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctgcc                                   <  1:1067717/139‑1 (MQ=255)
                                                                                           aCGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctgcc                                   <  2:1183073/139‑1 (MQ=255)
                                                                                              cgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      <  2:1056668/53‑1 (MQ=255)
                                                                                              cgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGaaa                                                                                                                      >  1:1056668/1‑53 (MQ=255)
                                                                                              cgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatgat                                                                                                    <  1:333323/71‑1 (MQ=255)
                                                                                              cgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatgat                                                                                                    >  2:333323/1‑71 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcg                                                                                                                               >  1:765550/1‑41 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAgcg                                                                                                                               <  2:765550/41‑1 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  1:274618/1‑66 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      <  2:274618/66‑1 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                             >  2:464378/1‑139 (MQ=255)
                                                                                                 cacaTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAAt                             >  1:672716/1‑139 (MQ=255)
                                                                                                    aTTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGa                          <  1:1032942/139‑1 (MQ=255)
                                                                                                      ttGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctgc                                    >  1:1341123/1‑127 (MQ=255)
                                                                                                      ttGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgcctgc                                    <  2:1341123/127‑1 (MQ=255)
                                                                                                      ttGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATc                        <  2:807090/139‑1 (MQ=255)
                                                                                                        gCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCtt                      <  1:258645/139‑1 (MQ=255)
                                                                                                             aTCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGAc                                                            <  2:659182/96‑1 (MQ=255)
                                                                                                             aTCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGAc                                                            >  1:659182/1‑96 (MQ=255)
                                                                                                              tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATca                <  2:408233/139‑1 (MQ=255)
                                                                                                              tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATca                <  1:50999/139‑1 (MQ=255)
                                                                                                              tCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATca                <  2:1029934/139‑1 (MQ=255)
                                                                                                                       tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         >  2:907483/1‑41 (MQ=255)
                                                                                                                       tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                         <  1:907483/41‑1 (MQ=255)
                                                                                                                       tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                         >  1:1500923/1‑73 (MQ=255)
                                                                                                                       tCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgc                                                                         <  2:1500923/73‑1 (MQ=255)
                                                                                                                        ccAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGa      <  2:174012/139‑1 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           <  2:993024/37‑1 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGc                                                                                                           >  1:993024/1‑37 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      <  1:121939/42‑1 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                      >  2:121939/1‑42 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc                                                                                           <  2:631744/53‑1 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGcc                                                                                           >  1:631744/1‑53 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc                                                      >  1:1462399/1‑90 (MQ=255)
                                                                                                                         cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAAc                                                      <  2:1462399/90‑1 (MQ=255)
                                                                                                                           gAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAg   <  1:464378/139‑1 (MQ=255)
                                                                                                                            aaaGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAgg  <  1:1105474/139‑1 (MQ=255)
                                                                                                                                cgcTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGgcgc                                                                       <  1:995072/66‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
AAGCAGCCAATCTCTTTTCTGGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGACAGG  >  NC_000913/2248649‑2248911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: