| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,426,492 | A→G | R99R (CGT→CGC) | hisJ ← | histidine ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,426,492 | 0 | A | G | 100.0% | 146.6 / NA | 40 | R99R (CGT→CGC) | hisJ | histidine ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/21); total (19/21) | |||||||||||
GGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAG > NC_000913/2426359‑2426626 | gggTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGttt < 2:466487/139‑1 (MQ=255) tgCCCTGCAATACGCCTTCCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGt > 2:757896/1‑139 (MQ=255) actACGCCTACCCGTTTGCTTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGGCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGaaa < 2:390128/137‑1 (MQ=255) cTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGa > 2:1370939/1‑139 (MQ=255) ttCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCaa < 1:1482087/139‑1 (MQ=255) tCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAAt > 1:1421897/1‑139 (MQ=255) actcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATcttctt < 1:846381/139‑1 (MQ=255) actcgactGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATcttctt > 2:846381/1‑139 (MQ=255) cGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa > 2:646240/1‑99 (MQ=255) cGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa < 1:646240/99‑1 (MQ=255) tGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACg > 1:1567/1‑139 (MQ=255) aaTGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACggg > 1:196862/1‑139 (MQ=255) ccaAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTc < 2:1567/139‑1 (MQ=255) aaaCGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 2:402489/139‑1 (MQ=255) aaaCGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 1:757896/139‑1 (MQ=255) aaaCGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 1:177916/139‑1 (MQ=255) aCGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTCCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACa < 1:799655/139‑1 (MQ=255) gaATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa > 1:347651/1‑133 (MQ=255) gaATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 2:347651/133‑1 (MQ=255) aaTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa < 1:63304/132‑1 (MQ=255) aaTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGa > 2:63304/1‑132 (MQ=255) aaTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGt < 1:566796/139‑1 (MQ=255) aaTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGt > 2:347685/1‑139 (MQ=255) aTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa > 2:613878/1‑60 (MQ=255) aTCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa < 1:613878/60‑1 (MQ=255) aGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGc > 1:874660/1‑139 (MQ=255) cGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATcttctt < 2:221575/82‑1 (MQ=255) cGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATcttctt > 1:221575/1‑82 (MQ=255) cGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGAc > 2:1210172/1‑95 (MQ=255) cGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGAc < 1:1210172/95‑1 (MQ=255) tACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa > 2:1174367/1‑50 (MQ=255) tACAGTTTGTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGa < 1:1174367/50‑1 (MQ=255) gTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGt > 2:247446/1‑139 (MQ=255) gTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGt < 2:196862/139‑1 (MQ=255) gTCGGTGAAGGCTATTTCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGt < 2:874660/139‑1 (MQ=255) ttCTTGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCttt > 1:662185/1‑139 (MQ=255) tGCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCa < 1:247446/139‑1 (MQ=255) gCTGGCGTTTTTCCGTAATGGAAAGCGATGACatg < 2:566517/35‑1 (MQ=255) gCTGGCGTTTTTCCGTAATGGAAAGCGATGACatg > 1:566517/1‑35 (MQ=255) gCTGGCGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAg > 2:1257586/1‑139 (MQ=255) | GGGTGGTGCCCTGCAATACGCCTACCCGTTTGCCTTTCAGCGACTCGACTGTCGGCTGAATGTCAGAATTTTTCGCCACCACCAAACGAGAATCGGCAGCGTACAGTTTGTCGGTGAAGGCTATTTCTTGCTGACGTTTTTCCGTAATGGAAAGCGATGACATGATGGCGTCAATCTTCTTCGCTTTTAAGGACGGGATTAACGCATCCAGCGGATTTTCGACAAACGTACATTGCGTATTGATGCGTTTGCATAATTCCTTTGCCAG > NC_000913/2426359‑2426626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |