Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	505,757	G→A	G282S (GGC→AGC)	ushA →	bifunctional UDP‑sugar hydrolase/5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	505,757	0	G	A	100.0%	156.8 / NA	46	G282S (GGC→AGC)	ushA	bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

GCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACGGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGAAAGT  >  NC_000913/505622‑505881                                                                                                                                        |                                                                                                                             gCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCa                                                                                                                           >  2:503245/1‑139 (MQ=255) gCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCa                                                                                                                           >  2:2527/1‑139 (MQ=255)   aCGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATc                                                                                                                         >  2:96508/1‑139 (MQ=255)              ctgccGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCa                                                                                                              <  2:1105990/139‑1 (MQ=255)                 ccGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGgc                                                                                                           >  1:294628/1‑139 (MQ=255)                       cGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATtgtg                                                                                                                <  2:6070/128‑1 (MQ=255)                       cGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATtgtg                                                                                                                >  1:6070/1‑128 (MQ=255)                       cGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGa                                                                                                     >  1:1476154/1‑139 (MQ=255)                          tGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTg                                                                                                  <  1:503245/139‑1 (MQ=255)                              gatgatCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAAC‑AAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCa                                                                                             <  1:509091/139‑1 (MQ=255)                                      tcgGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAg                                                                                                    <  2:1432191/125‑1 (MQ=255)                                      tcgGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAg                                                                                                    >  1:1432191/1‑125 (MQ=255)                                          tggtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGa                                                                                  <  1:407034/139‑1 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACTCTATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGTGTGGGGCAAATACGTGGGACg                                                                                >  2:878779/1‑139 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGc                                                                                                               >  2:221/1‑108 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGc                                                                                                               <  1:221/108‑1 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGt                                                                                                   >  1:598178/1‑120 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGt                                                                                                   <  2:598178/120‑1 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACg                                                                                >  1:1490020/1‑139 (MQ=255)                                            gtCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACg                                                                                <  2:1476154/139‑1 (MQ=255)                                              cACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACggg                                                                              <  2:294628/139‑1 (MQ=255)                                                      aGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGAtttt                                                                      <  1:762934/139‑1 (MQ=255)                                                      aGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGAtttt                                                                      <  2:9266/139‑1 (MQ=255)                                                             gTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAg                                                                                                    <  1:488699/102‑1 (MQ=255)                                                             gTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAg                                                                                                    >  2:488699/1‑102 (MQ=255)                                                                       cggcAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGa                                                     >  1:1038401/1‑139 (MQ=255)                                                                                   aaaaaCAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGCTTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGt                                         <  2:1078940/139‑1 (MQ=255)                                                                                   aaaaaCAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGt                                         <  1:5891/139‑1 (MQ=255)                                                                                              gATTACGTGCCGGGTACGCCAGGCAAAACAGATCAAAAAAACAGCATCTGGATTGTGCAGGCGGATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGc                              <  1:889221/139‑1 (MQ=255)                                                                                                          ggTACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATGCATGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTGACTACCAGCTGATTCCGGTGa                  >  2:878490/1‑139 (MQ=255)                                                                                                            tACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGc                                                                             <  2:62072/78‑1 (MQ=255)                                                                                                            tACGCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGc                                                                             >  1:62072/1‑78 (MQ=255)                                                                                                               gCCATGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTg             <  1:1260621/139‑1 (MQ=255)                                                                                                                   tGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGtt                                        <  2:150633/108‑1 (MQ=255)                                                                                                                   tGCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGtt                                        >  1:150633/1‑108 (MQ=255)                                                                                                                    gCAAACCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTgaagaa        >  2:1312916/1‑139 (MQ=255)                                                                                                                        aCCAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGaaa    <  2:1490020/139‑1 (MQ=255)                                                                                                                          cAGATCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGAAAGt  >  2:671540/1‑139 (MQ=255)                                                                                                                             aTCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGc                                                                             <  1:1412242/61‑1 (MQ=255)                                                                                                                             aTCAACAAAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGc                                                                             >  2:1412242/1‑61 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGtt                                        <  1:889001/93‑1 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGtt                                        >  2:889001/1‑93 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTa                                       >  2:167018/1‑94 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTa                                       <  1:167018/94‑1 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGAAAGt  <  1:1321649/131‑1 (MQ=255)                                                                                                                                  caaAACAGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGAAAGt  >  2:1321649/1‑131 (MQ=255)                                                                                                                                        |                                                                                                                             GCACGCGCGCTGCCTGCCGGATCGCTGGCGATGATCGTCGGTGGTCACTCGCAAGATCCGGTCTGCATGGCGGCAGAAAACAAAAAACAGGTCGATTACGTGCCGGGTACGCCATGCAAACCAGATCAACAAAACGGCATCTGGATTGTGCAGGCGCATGAGTGGGGCAAATACGTGGGACGGGCTGATTTTGAGTTTCGTAATGGCGAAATGAAAATGGTTAACTACCAGCTGATTCCGGTGAACCTGAAGAAGAAAGT  >  NC_000913/505622‑505881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑