Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,133	C→T	V185I (GTC→ATC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,133	0	C	T	100.0%	189.6 / NA	55	V185I (GTC→ATC)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)

TTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2909999‑2910267                                                                                                                                       |                                                                                                                                       ttCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGataaca                                                                                                                                    <  2:1480967/139‑1 (MQ=255)   cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  >  1:1090861/1‑139 (MQ=255)   cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  >  2:207384/1‑139 (MQ=255)      gAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTg                                                                                                                               >  2:1113870/1‑139 (MQ=255)        aaaCGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGcc                                                                                                                             <  1:419047/139‑1 (MQ=255)           acgcccttcaaTTTTTCCTTCCCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          <  2:792843/130‑1 (MQ=255)                                  tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  2:1410575/139‑1 (MQ=255)                                  tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  1:515606/139‑1 (MQ=255)                                          aTGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTa                                                                                           <  1:207384/139‑1 (MQ=255)                                            gCGATTTTTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGa                                                                                         >  2:988433/1‑139 (MQ=255)                                            gCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGa                                                                                         <  1:1113870/139‑1 (MQ=255)                                                 ttCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTa                                                                                    <  2:1090861/139‑1 (MQ=255)                                                     gCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGa                                                                                >  1:1360478/1‑139 (MQ=255)                                                              ccTAAATGGCGAGCCAGATTAACCGTGACAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       >  1:1478399/1‑139 (MQ=255)                                                                      gCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtc                                                               >  2:784548/1‑139 (MQ=255)                                                                       cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcg                                                              >  1:1151629/1‑139 (MQ=255)                                                                       cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcg                                                              >  2:62943/1‑139 (MQ=255)                                                                       cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGAACCAAGcgtcg                                                              >  1:976807/1‑139 (MQ=255)                                                                         aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  2:876395/100‑1 (MQ=255)                                                                         aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   >  1:876395/1‑100 (MQ=255)                                                                         aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCCGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCgtcgtc                                                            <  1:802643/139‑1 (MQ=255)                                                                                    ccGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGcc                                                 <  1:625735/139‑1 (MQ=255)                                                                                        ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTc                                                                                                                      <  2:1106483/66‑1 (MQ=255)                                                                                        ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTc                                                                                                                      >  1:1106483/1‑66 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        <  2:231196/63‑1 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        >  1:231196/1‑63 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  <  1:991252/101‑1 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  >  2:991252/1‑101 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       >  1:1266563/1‑112 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       <  2:1266563/112‑1 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAAt                                                        >  1:118623/1‑127 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAAt                                                        <  2:118623/127‑1 (MQ=255)                                                                                         gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGt                                            >  1:872393/1‑139 (MQ=255)                                                                                                gcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCa                                     <  2:1360478/139‑1 (MQ=255)                                                                                                 cagGTCCCCCATTTCCTCCTCCGGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCaa                                    <  1:531965/139‑1 (MQ=255)                                                                                                 cagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCaa                                    <  2:872393/139‑1 (MQ=255)                                                                                                    gTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGt                                 >  1:1090735/1‑139 (MQ=255)                                                                                                      cccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGttt                               >  1:925321/1‑139 (MQ=255)                                                                                                       ccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTc                              >  2:820145/1‑139 (MQ=255)                                                                                                         ccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAg                                                                                          <  1:458047/77‑1 (MQ=255)                                                                                                         ccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAg                                                                                          >  2:458047/1‑77 (MQ=255)                                                                                                                  cctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTAc                                                                            <  2:97041/82‑1 (MQ=255)                                                                                                                  cctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTAc                                                                            >  1:97041/1‑82 (MQ=255)                                                                                                                    tccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTgcg                 >  2:306192/1‑139 (MQ=255)                                                                                                                      cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  2:1151629/139‑1 (MQ=255)                                                                                                                      cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  1:784548/139‑1 (MQ=255)                                                                                                                         tttAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    >  2:1057192/1‑51 (MQ=255)                                                                                                                         tttAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    <  1:1057192/51‑1 (MQ=255)                                                                                                                            aGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCAtt         <  2:1478399/139‑1 (MQ=255)                                                                                                                              ccTGCTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       <  1:993371/139‑1 (MQ=255)                                                                                                                                  gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       <  2:266132/135‑1 (MQ=255)                                                                                                                                  gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       >  1:266132/1‑135 (MQ=255)                                                                                                                                  gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc   >  2:320371/1‑139 (MQ=255)                                                                                                                                  gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc   >  2:902113/1‑139 (MQ=255)                                                                                                                                   tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGGCTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg  >  2:358062/1‑139 (MQ=255)                                                                                                                                   tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg  >  1:1273970/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2909999‑2910267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑