Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,043,412	G→A	A71V (GCA→GTA)	yqfB ←	UPF0267 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,043,412	0	G	A	100.0%	156.3 / NA	45	A71V (GCA→GTA)	yqfB	UPF0267 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/22);  total (23/22)

AATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACTCGTCG  >  NC_000913/3043277‑3043546                                                                                                                                        |                                                                                                                                       aaTGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACAt                                                                                                                                     >  2:1352523/1‑139 (MQ=255)          tttCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            <  1:978397/139‑1 (MQ=255)            tCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcagtc                                                                                                                          <  2:1417314/139‑1 (MQ=255)                          tGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGt                                                                                                            <  1:1352523/139‑1 (MQ=255)                                        aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTg                                                                                              >  1:813843/1‑139 (MQ=255)                                          aCATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  1:92738/139‑1 (MQ=255)                                             tttAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTg                                                                                         >  1:685253/1‑139 (MQ=255)                                                      aaTCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGtt                                                                                                           <  2:1463145/112‑1 (MQ=255)                                                      aaTCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGtt                                                                                                           >  1:1463145/1‑112 (MQ=255)                                                      aaTCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATc                                                                                >  1:1104944/1‑139 (MQ=255)                                                           caTAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCa                                                                           >  1:5796/1‑139 (MQ=255)                                                            aTAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCaa                                                                          >  1:643555/1‑139 (MQ=255)                                                                  ttGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATaa                                                                    <  2:1104944/139‑1 (MQ=255)                                                                      gtCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                >  1:1031486/1‑139 (MQ=255)                                                                       tCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTc                                                               <  1:1293163/139‑1 (MQ=255)                                                                          gACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATc                                                            >  2:1279467/1‑139 (MQ=255)                                                                           aCCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            >  1:697775/1‑106 (MQ=255)                                                                           aCCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  2:697775/106‑1 (MQ=255)                                                                               ggATAGATGTCAGCAATGACTTTTTTCCGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCaa                                                       <  2:861403/139‑1 (MQ=255)                                                                                     aTGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTc                                                 <  2:813843/139‑1 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            >  1:621184/1‑59 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            <  2:621184/59‑1 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTc                                                               <  1:888994/120‑1 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTc                                                               >  2:888994/1‑120 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            >  2:680388/1‑139 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            <  2:1031486/139‑1 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            >  2:579091/1‑139 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCATATCCAGCGTTACGGTTGAGGTTGCg                                                                                            >  1:57861/1‑91 (MQ=255)                                                                                          aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCATATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  2:57861/91‑1 (MQ=255)                                                                                               tGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            >  1:21767/1‑86 (MQ=255)                                                                                               tGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  2:21767/86‑1 (MQ=255)                                                                                                               agtcagGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                <  1:432210/98‑1 (MQ=255)                                                                                                               agtcagGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                >  2:432210/1‑98 (MQ=255)                                                                                                               agtcagGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGc                                      >  1:1105646/1‑124 (MQ=255)                                                                                                               agtcagGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGc                                      <  2:1105646/124‑1 (MQ=255)                                                                                                                   agGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGt                   <  1:1279467/139‑1 (MQ=255)                                                                                                                         atatTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGAtt             <  1:680388/139‑1 (MQ=255)                                                                                                                             tttCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGa         <  1:579091/139‑1 (MQ=255)                                                                                                                               tCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGtt                                                                                                           >  2:291084/1‑39 (MQ=38)                                                                                                                               tCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGtt                                                                                                           <  1:291084/39‑1 (MQ=38)                                                                                                                               tCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACt       >  2:1445875/1‑139 (MQ=255)                                                                                                                               tCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACt       >  2:693650/1‑139 (MQ=255)                                                                                                                                    ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGt                                                                                          >  1:1179726/1‑51 (MQ=255)                                                                                                                                    ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGt                                                                                          <  2:1179726/51‑1 (MQ=255)                                                                                                                                    ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACtcgtcg  >  1:1470211/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       AATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACTCGTCG  >  NC_000913/3043277‑3043546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑