Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,930	G→A	D100N (GAT→AAT)	ebgA →	evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,930	0	G	A	100.0%	230.4 / NA	67	D100N (GAT→AAT)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/31);  total (36/31)

ATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAA  >  NC_000913/3222796‑3223063                                                                                                                                       |                                                                                                                                      aTCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAAtgt                                                                                                                                   <  2:1043546/139‑1 (MQ=255)             tACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTccc                                                                                                                       >  2:992160/1‑139 (MQ=255)              aCCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTcccc                                                                                                                      >  2:112585/1‑139 (MQ=255)                    aGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGAt                                                                                                                <  1:531763/139‑1 (MQ=255)                       cTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGCGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAAc                                                                                                             >  1:1017673/1‑139 (MQ=255)                        ttCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAAcc                                                                                                            >  2:578610/1‑139 (MQ=255)                            ccTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAAc                                                                                                        <  1:1048785/139‑1 (MQ=255)                                tGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGt                                                                                                    >  1:905761/1‑139 (MQ=255)                                       aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGTCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATc                                                                                             >  1:751511/1‑139 (MQ=255)                                       aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGc                                                                                                  >  2:434411/1‑134 (MQ=255)                                       aTGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGc                                                                                                  <  1:434411/134‑1 (MQ=255)                                           cTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                         <  1:112585/139‑1 (MQ=255)                                           cTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACg                                                                                         <  1:646404/139‑1 (MQ=255)                                             gACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTa                                                                                       >  2:1471934/1‑139 (MQ=255)                                              aCTGGGGGCATATTACCGTCCCCGCCATGTGTCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCg                                                                                                                  >  2:1262398/1‑111 (MQ=255)                                              aCTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCg                                                                                                                  <  1:1262398/111‑1 (MQ=255)                                              aCTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTAt                                                                                      >  2:415198/1‑139 (MQ=255)                                                              cGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCa                                                                                            <  1:1428158/117‑1 (MQ=255)                                                              cGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCa                                                                                            >  2:1428158/1‑117 (MQ=255)                                                                  cccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTccc                                                                                                                       >  2:1375872/1‑86 (MQ=255)                                                                  cccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTccc                                                                                                                       <  1:1375872/86‑1 (MQ=255)                                                                   ccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGTCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGATGGCTg                                                                 <  2:751511/139‑1 (MQ=255)                                                                   ccGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTg                                                                 >  2:1172041/1‑139 (MQ=255)                                                                    cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTg                                                                 <  1:1251426/138‑1 (MQ=255)                                                                    cGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTg                                                                 >  2:1251426/1‑138 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCTATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGc                                                               >  1:738105/1‑139 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCg                                                                                                      <  1:802659/100‑1 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCg                                                                                                      >  2:802659/1‑100 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTgg                                                                <  1:600831/138‑1 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTgg                                                                >  2:600831/1‑138 (MQ=255)                                                                     gCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGc                                                               >  1:833406/1‑139 (MQ=255)                                                                         tgtgGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAggg                                                           <  1:415198/139‑1 (MQ=255)                                                                           tgGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTAt                                                                                              >  1:1250838/1‑102 (MQ=255)                                                                           tgGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTAt                                                                                              <  2:1250838/102‑1 (MQ=255)                                                                                         tCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGAtt                                           >  2:128736/1‑139 (MQ=255)                                                                                           aCGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGttt                                                                                                                            >  1:1149701/1‑56 (MQ=255)                                                                                           aCGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGttt                                                                                                                            <  2:1149701/56‑1 (MQ=255)                                                                                            cGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACg                                                 >  1:1097833/1‑130 (MQ=255)                                                                                            cGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACg                                                 <  2:1097833/130‑1 (MQ=255)                                                                                            cGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTaaa                                        >  1:1374332/1‑139 (MQ=255)                                                                                                   cTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACg                                 >  2:232565/1‑139 (MQ=255)                                                                                                   cTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACg                                 <  2:1017673/139‑1 (MQ=255)                                                                                                           taCCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGaa                         <  1:992160/139‑1 (MQ=255)                                                                                                           taCCGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGaa                         >  2:1101162/1‑139 (MQ=255)                                                                                                             ccGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAAc                       <  1:128736/139‑1 (MQ=255)                                                                                                             ccGACGAAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAAc                       <  1:232565/139‑1 (MQ=255)                                                                                                              cgacgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAAcc                      >  1:1373953/1‑139 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAAcc                                                                                                       >  1:546494/1‑56 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAAcc                                                                                                       <  2:546494/56‑1 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCa                                                                                 >  1:590903/1‑78 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCa                                                                                 <  2:590903/78‑1 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGaa                         <  2:1442330/134‑1 (MQ=255)                                                                                                                acgaAGGTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGaa                         >  1:1442330/1‑134 (MQ=255)                                                                                                                     ggTTTTCCGTTCCCCATCAATGTGCCGTTTGTcccc                                                                                                                      <  2:151073/36‑1 (MQ=255)                                                                                                                     ggTTTTCCGTTCCCCATCAATGTGCCGTTTGTcccc                                                                                                                      >  1:151073/1‑36 (MQ=255)                                                                                                                     ggTTTTCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTg               >  2:1061491/1‑139 (MQ=255)                                                                                                                       ttttCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGaa             <  2:1374332/139‑1 (MQ=255)                                                                                                                         ttCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGt           >  2:396740/1‑139 (MQ=255)                                                                                                                          tCCGTTCCCCATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTTCCTATCTACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAACTTTGACGGCGTCGACACCTATTTTGTAGTc          >  2:571972/1‑139 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAggg                                                           <  2:76168/84‑1 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAggg                                                           >  1:76168/1‑84 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTAttt                 >  1:1155095/1‑126 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTAttt                 <  2:1155095/126‑1 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGtt    <  1:1101162/139‑1 (MQ=255)                                                                                                                                ccccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGtt    >  2:7903/1‑139 (MQ=255)                                                                                                                                  ccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTaa  <  1:1471934/139‑1 (MQ=255)                                                                                                                                  ccATCAATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTaa  <  2:905761/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ATCCGCTGCAAGTACCAGAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGGCAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGATGTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGCGACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAA  >  NC_000913/3222796‑3223063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑