Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,135	T→C	I142I (ATT→ATC)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,135	0	T	C	100.0%	165.8 / NA	46	I142I (ATT→ATC)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/27);  total (19/27)

TGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAG  >  NC_000913/535999‑536268                                                                                                                                         |                                                                                                                                      tGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGa                                                                                                                                     <  1:524362/139‑1 (MQ=255)   ggTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAAc                                                                                                                                   >  1:1178137/1‑139 (MQ=255)   ggTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAAc                                                                                                                                   >  2:1018238/1‑139 (MQ=255)         aaaaaaaTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTa                                                                                                                             <  1:967610/139‑1 (MQ=255)          aaaaaaTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTaa                                                                                                                            <  2:323576/139‑1 (MQ=255)             aaaTTAACTGTCTGGCAGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCa                                                                                                                         >  2:527433/1‑139 (MQ=255)               aTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCTAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCAtt                                                                                                                       <  1:1018238/139‑1 (MQ=255)                 tAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCAtttt                                                                                                                     <  2:1178137/139‑1 (MQ=255)                  aaCTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTg                                                                                                                    <  2:780130/139‑1 (MQ=255)                     tGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGata                                                                                                                 <  2:750035/139‑1 (MQ=255)                       tCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATAtt                                                                                                               >  2:1030629/1‑139 (MQ=255)                              ggTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGtt                                                                                                        >  1:214619/1‑139 (MQ=255)                                 aaaaCGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTcc                                                                                                     >  2:965893/1‑139 (MQ=255)                                 aaaaCGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTcc                                                                                                     >  2:332778/1‑139 (MQ=255)                                        cGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCAc                                                                                              <  2:1311769/139‑1 (MQ=255)                                                    gcagTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCa                                                                                  <  1:653644/139‑1 (MQ=255)                                                        tGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcg                                                                              >  2:293759/1‑139 (MQ=255)                                                          aaCAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCt                                                                            >  1:76441/1‑139 (MQ=255)                                                                   aCGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgat                                                                   <  2:214619/139‑1 (MQ=255)                                                                      cAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAAttgattga                                                                <  2:913491/139‑1 (MQ=255)                                                                                   aaaCCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTAt                                                                                                                               >  1:516318/1‑63 (MQ=255)                                                                                   aaaCCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTAt                                                                                                                               <  2:516318/63‑1 (MQ=255)                                                                                         gCGTTATGCCGCGAATATGCTGATGAAAGAGGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgattg                                                                 <  2:1180680/119‑1 (MQ=255)                                                                                         gCGTTATGCCGCGAATATGCTGATGAAAGAGGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgattg                                                                 >  1:1180680/1‑119 (MQ=255)                                                                                                gCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCAGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaaaa                                      <  1:965893/139‑1 (MQ=255)                                                                                                    cgAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTaa                                                                                                                            >  2:571110/1‑49 (MQ=255)                                                                                                    cgAATATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTaa                                                                                                                            <  1:571110/49‑1 (MQ=255)                                                                                                      aaTATGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGt                                >  1:951028/1‑139 (MQ=255)                                                                                                       atatGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAAtt                                    <  2:342906/134‑1 (MQ=255)                                                                                                       atatGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAAtt                                    >  1:342906/1‑134 (MQ=255)                                                                                                       atatGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTa                               <  2:332623/139‑1 (MQ=255)                                                                                                        tatGCTGATGAAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTAt                              <  2:414493/139‑1 (MQ=255)                                                                                                             tgatgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAt                         <  1:332778/139‑1 (MQ=255)                                                                                                             tgatgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAt                         <  2:76441/139‑1 (MQ=255)                                                                                                               atgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCAt                                                                                                   <  2:153229/63‑1 (MQ=255)                                                                                                               atgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCAt                                                                                                   >  1:153229/1‑63 (MQ=255)                                                                                                               atgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATCGATGATGTTGGTTGCTGCAATTTGAAAAtt                                    <  1:1341636/126‑1 (MQ=255)                                                                                                               atgaAAGAAGATATTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATCGATGATGTTGGTTGCTGCAATTTGAAAAtt                                    >  2:1341636/1‑126 (MQ=255)                                                                                                                         atatTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAt                         >  1:492458/1‑127 (MQ=255)                                                                                                                         atatTTTATTACTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAt                         <  2:492458/127‑1 (MQ=255)                                                                                                                              ttattaCTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGa        <  2:342568/139‑1 (MQ=255)                                                                                                                               tattaCTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGAt       <  1:1030629/139‑1 (MQ=255)                                                                                                                                    cTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAg  <  1:527433/139‑1 (MQ=255)                                                                                                                                    cTGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAg  >  2:847346/1‑139 (MQ=255)                                                                                                                                     tGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTAt                              <  2:1367114/110‑1 (MQ=255)                                                                                                                                     tGATCGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTAt                              >  1:1367114/1‑110 (MQ=255)                                                                                                                                         |                                                                                                                                      TGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAG  >  NC_000913/535999‑536268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑