Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,388,394 T→C M245V (ATG→GTG)  aaeA ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,388,3940TC100.0% 200.5 / NA 54M245V (ATG→GTG) aaeAp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/26);  total (28/26)

CCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAG  >  NC_000913/3388267‑3388528
                                                                                                                               |                                                                                                                                      
ccAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTgtcgt                                                                                                                             <  1:751836/139‑1 (MQ=255)
    tGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcg                                                                                                                         >  2:778157/1‑139 (MQ=255)
      accaccACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcgcg                                                                                                                       <  2:643093/139‑1 (MQ=255)
      accaccACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcgcg                                                                                                                       <  1:1100897/139‑1 (MQ=255)
      accaccACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcgcg                                                                                                                       >  1:643093/1‑139 (MQ=255)
            acTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGtgctgc                                                                                                                 <  1:571478/139‑1 (MQ=255)
               gTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTgg                                                                                                              <  1:361865/139‑1 (MQ=255)
                tAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGc                                                                                                             >  2:1001514/1‑139 (MQ=255)
                        gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGt                                                                                                                      <  1:813685/122‑1 (MQ=255)
                        gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGt                                                                                                                      >  2:813685/1‑122 (MQ=255)
                        gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTg                                                                                                      <  2:177146/138‑1 (MQ=255)
                        gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTg                                                                                                      >  1:177146/1‑138 (MQ=255)
                          gCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcc                                                                                                   >  1:292128/1‑139 (MQ=255)
                           cccGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAccc                                                                                                  >  2:749793/1‑139 (MQ=255)
                                   ccAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCa                                                                                          <  1:1001514/139‑1 (MQ=255)
                                   ccAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCa                                                                                          <  2:590355/139‑1 (MQ=255)
                                    cAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCaa                                                                                         <  1:537843/139‑1 (MQ=255)
                                            ctctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCa                                                                                 >  2:1150155/1‑139 (MQ=255)
                                                 gCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGt                                                                            >  2:967633/1‑139 (MQ=255)
                                                  cTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGATAGAGTCTATAGTCGCCACACCGTTAGCTTCACGCGTGCTGCTGGCGTTGGATACCCCTGCGGCAACACTATCAACAGTc                                                                           <  2:164956/139‑1 (MQ=255)
                                                    ggTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTccc                                                                         >  2:21941/1‑139 (MQ=255)
                                                    ggTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTccc                                                                         >  1:235346/1‑139 (MQ=255)
                                                     gTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCt                                                                        >  1:1404270/1‑139 (MQ=255)
                                                       tGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCttt                                                                      <  2:1402073/139‑1 (MQ=255)
                                                         tCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcccc                                                                                                 <  2:548671/110‑1 (MQ=255)
                                                         tCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGAcccc                                                                                                 >  1:548671/1‑110 (MQ=255)
                                                          cGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAg                                                                   <  1:734929/139‑1 (MQ=255)
                                                          cGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAg                                                                   <  1:1150155/139‑1 (MQ=255)
                                                                   tACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCaa                                                                                         <  1:187977/108‑1 (MQ=255)
                                                                   tACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCaa                                                                                         >  2:187977/1‑108 (MQ=255)
                                                                         ccGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGcc                                                    <  2:1404270/139‑1 (MQ=255)
                                                                             aaCACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAgc                                                >  2:421740/1‑139 (MQ=255)
                                                                                     gcgcAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGAt                                        >  1:631129/1‑139 (MQ=255)
                                                                                      cgcAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGAtc                                       >  2:1364459/1‑139 (MQ=255)
                                                                                       gcAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcgcg                                                                                                                       <  2:298202/58‑1 (MQ=255)
                                                                                       gcAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcgcg                                                                                                                       >  1:298202/1‑58 (MQ=255)
                                                                                         aaGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATctct                                    <  1:1364459/139‑1 (MQ=255)
                                                                                         aaGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATctct                                    <  1:749793/139‑1 (MQ=255)
                                                                                           gACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGc                                  >  1:1304709/1‑139 (MQ=255)
                                                                                            aCGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCa                                 <  1:21941/139‑1 (MQ=255)
                                                                                             cGCACCCATTCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCAc                                <  2:292128/139‑1 (MQ=255)
                                                                                                     ttCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAac                                                                                        <  2:678058/75‑1 (MQ=255)
                                                                                                     ttCAAGGTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAac                                                                                        >  1:678058/1‑75 (MQ=255)
                                                                                                          ggTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcg                                                                                                                         <  1:1083095/37‑1 (MQ=255)
                                                                                                          ggTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCAcg                                                                                                                         >  2:1083095/1‑37 (MQ=255)
                                                                                                          ggTTAGAGTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACg                   >  2:645575/1‑139 (MQ=255)
                                                                                                              agagTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGAtct                                      >  2:705788/1‑116 (MQ=255)
                                                                                                              agagTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGAtct                                      <  1:705788/116‑1 (MQ=255)
                                                                                                               gagTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCAccc              >  1:858242/1‑139 (MQ=255)
                                                                                                               gagTCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCAccc              >  2:973805/1‑139 (MQ=255)
                                                                                                                  tCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCgg                                                                                            <  2:769584/58‑1 (MQ=255)
                                                                                                                  tCTATAGTCGCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCgg                                                                                            >  1:769584/1‑58 (MQ=255)
                                                                                                                           gCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAg  >  2:493163/1‑139 (MQ=255)
                                                                                                                           gCCACCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAg  >  2:944324/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                      
CCAGTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGTCACGCGTGCTGCTGGCGTTGGTGACCCCTGCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAG  >  NC_000913/3388267‑3388528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑