Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,125 T→C T37A (ACC→GCC)  rplV ← 50S ribosomal subunit protein L22

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1250TC100.0% 198.9 / NA 55T37A (ACC→GCC) rplV50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/30);  total (25/30)

TGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTC  >  NC_000913/3449990‑3450260
                                                                                                                                       |                                                                                                                                       
tGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaa                                                                                                                                      <  1:1190963/139‑1 (MQ=255)
 gCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaa                                                                                                                                     <  2:456565/139‑1 (MQ=255)
 gCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaa                                                                                                                                     <  2:909152/139‑1 (MQ=255)
      ggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCGGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATc                                                                                                                                >  1:98559/1‑139 (MQ=255)
      ggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTAACCAGTACAGCCGCGTTCTTGTTGGTGTAGGCCAAAATATc                                                                                                                                >  2:1351906/1‑139 (MQ=255)
            cGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGc                                                                                                                          >  1:1235027/1‑139 (MQ=255)
             gTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGcc                                                                                                                         >  2:367644/1‑139 (MQ=255)
              tCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCt                                                                                                                        <  2:68569/139‑1 (MQ=255)
                  cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                                    <  2:818614/139‑1 (MQ=255)
                         tttCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTGCAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTc                                                                                                             <  2:1469133/139‑1 (MQ=255)
                                  tttCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGa                                                                                                    >  1:795628/1‑139 (MQ=255)
                                    tCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  2:1422756/139‑1 (MQ=255)
                                    tCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAACCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  1:657514/139‑1 (MQ=255)
                                       gATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAg                                                                                               <  2:572529/139‑1 (MQ=255)
                                                  tCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaacaa                                                                                    <  2:710972/139‑1 (MQ=255)
                                                            cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTg                                                                                                                       >  1:1418415/1‑94 (MQ=255)
                                                            cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTg                                                                                                                       <  2:1418415/94‑1 (MQ=255)
                                                                 tgtTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAg                                                                     <  1:1351906/139‑1 (MQ=255)
                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  >  1:414271/1‑106 (MQ=255)
                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  2:414271/106‑1 (MQ=255)
                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAAAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCaga                                                                 >  2:924094/1‑139 (MQ=255)
                                                                        cGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCagaaga                                                              >  1:1245985/1‑139 (MQ=255)
                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              <  1:571416/72‑1 (MQ=255)
                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              >  2:571416/1‑72 (MQ=255)
                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  2:1296134/119‑1 (MQ=255)
                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  1:1296134/1‑119 (MQ=255)
                                                                             gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                            <  1:58704/72‑1 (MQ=255)
                                                                             gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                            >  2:58704/1‑72 (MQ=255)
                                                                                    cAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCg                                                  <  1:864473/139‑1 (MQ=255)
                                                                                    cAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCg                                                  <  1:367644/139‑1 (MQ=255)
                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              >  1:34424/1‑60 (MQ=255)
                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              <  2:34424/60‑1 (MQ=255)
                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               >  1:964283/1‑139 (MQ=255)
                                                                                         tCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGtt                                             >  1:73411/1‑139 (MQ=255)
                                                                                                   tCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGt                                   >  2:1438617/1‑139 (MQ=255)
                                                                                                             tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  2:1366922/1‑85 (MQ=255)
                                                                                                             tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  1:1366922/85‑1 (MQ=255)
                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         <  2:1103339/102‑1 (MQ=255)
                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         >  1:1103339/1‑102 (MQ=255)
                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTtcctc                    <  2:878866/139‑1 (MQ=255)
                                                                                                                      tttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAc                <  2:795628/139‑1 (MQ=255)
                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  2:538599/1‑75 (MQ=255)
                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  1:538599/75‑1 (MQ=255)
                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt                                                                        >  2:1153533/1‑82 (MQ=255)
                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt                                                                        <  1:1153533/82‑1 (MQ=255)
                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAcc               >  1:1033100/1‑139 (MQ=255)
                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         >  2:1019279/1‑90 (MQ=255)
                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttc        <  1:1438617/139‑1 (MQ=255)
                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTATTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         <  1:1019279/90‑1 (MQ=255)
                                                                                                                               ggTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                    >  2:1329186/1‑78 (MQ=255)
                                                                                                                               ggTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                    <  1:1329186/78‑1 (MQ=255)
                                                                                                                                gtgtAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttctt      >  1:1151889/1‑139 (MQ=255)
                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               >  2:1431193/1‑94 (MQ=255)
                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               <  1:1431193/94‑1 (MQ=255)
                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttcttcttc  <  2:1033100/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
TGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTC  >  NC_000913/3449990‑3450260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑