Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,469,498	T→C	Q119R (CAG→CGG)	chiA ←	periplasmic endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,469,498	0	T	C	100.0%	311.8 / NA	83	Q119R (CAG→CGG)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/35);  total (48/35)

CACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAC  >  NC_000913/3469367‑3469633                                                                                                                                    |                                                                                                                                        cACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTgg                                                                                                                                  >  2:104960/1‑139 (MQ=255)        aCAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGcc                                                                                                                           <  2:1434101/139‑1 (MQ=255)              agaagaGGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCg                                                                                                                     >  2:470419/1‑139 (MQ=255)                    ggCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTa                                                                                                               >  1:1169395/1‑139 (MQ=255)                      cTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGc                                                                                                             >  1:326853/1‑139 (MQ=255)                      cTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGc                                                                                                             >  1:218621/1‑139 (MQ=255)                          ccaccaGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         <  1:470419/139‑1 (MQ=255)                               aGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTAtt                                                                                                    <  2:1169395/139‑1 (MQ=255)                                     ttttATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGc                                                                                              <  2:218621/139‑1 (MQ=255)                                             aCCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATACAGGCGTat                                                                                      >  2:465783/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       >  2:25733/1‑104 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       <  1:25733/104‑1 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         >  2:396306/1‑118 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         <  1:396306/118‑1 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAgg                                                                                           >  2:803008/1‑132 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAgg                                                                                           <  1:803008/132‑1 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  1:317600/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  2:928332/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  2:816623/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  1:1368279/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  1:1051257/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  2:1302243/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  2:586651/1‑139 (MQ=255)                                               cGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTattt                                                                                    >  2:741424/1‑139 (MQ=255)                                                 cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGaa                                                                                                                        >  1:226195/1‑101 (MQ=255)                                                 cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGaa                                                                                                                        <  2:226195/101‑1 (MQ=255)                                                 cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAg                                                                                            <  1:1475385/129‑1 (MQ=255)                                                 cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAg                                                                                            >  2:1475385/1‑129 (MQ=255)                                                 cACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttga                                                                                  >  2:784150/1‑139 (MQ=255)                                                   ccATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTatttgaat                                                                                <  1:793353/139‑1 (MQ=255)                                                            acgacATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGaagaag                                                                       >  1:620258/1‑139 (MQ=255)                                                              gacATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGAt                                                                     >  2:1409471/1‑139 (MQ=255)                                                               acATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATg                                                                    <  1:784150/139‑1 (MQ=255)                                                               acATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATg                                                                    >  1:480567/1‑139 (MQ=255)                                                                   aCCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTa                                                                                                      >  1:787505/1‑101 (MQ=255)                                                                   aCCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTa                                                                                                      <  2:787505/101‑1 (MQ=255)                                                                        gTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGc                                                           >  2:602578/1‑139 (MQ=255)                                                                        gTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGc                                                           >  2:164694/1‑139 (MQ=255)                                                                             aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCttt                                                      >  2:740014/1‑139 (MQ=255)                                                                             aCCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCttt                                                      >  1:997304/1‑139 (MQ=255)                                                                               cGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTc                                                    <  2:432985/139‑1 (MQ=255)                                                                                gCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCg                                                   <  2:997304/139‑1 (MQ=255)                                                                                  ccTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCAt                                                                                                                 >  2:530711/1‑75 (MQ=255)                                                                                  ccTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCAt                                                                                                                 <  1:530711/75‑1 (MQ=255)                                                                                     gctgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGa                                              <  1:816623/139‑1 (MQ=255)                                                                                      ctgTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                             <  2:1368279/139‑1 (MQ=255)                                                                                              tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGTCGAACCGCCATTAGCCGGc                                                                                                         >  2:930619/1‑71 (MQ=255)                                                                                              tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         <  1:930619/71‑1 (MQ=255)                                                                                              tCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTAtt                                     <  2:317600/139‑1 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       <  2:1327620/55‑1 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       >  1:555406/1‑55 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       <  2:555406/55‑1 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAAc                                                                                                                       >  1:1327620/1‑55 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         >  2:994840/1‑69 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGc                                                                                                         <  1:994840/69‑1 (MQ=255)                                                                                                cAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGc                                   >  1:150361/1‑139 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGc                                                                                                             >  2:674134/1‑62 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGc                                                                                                             <  1:674134/62‑1 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                             >  2:1244640/1‑126 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                             <  1:1244640/126‑1 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaa                                <  2:1051257/139‑1 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATACAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaa                                <  1:465783/139‑1 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCACCATTAg                                                                                                              <  2:1399483/61‑1 (MQ=255)                                                                                                   gCAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCACCATTAg                                                                                                              >  1:1399483/1‑61 (MQ=255)                                                                                                    cAACTACAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCaaa                               <  1:451313/139‑1 (MQ=255)                                                                                                          cAGAAGAATTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACt                         >  1:696993/1‑139 (MQ=255)                                                                                                                 aTTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAg                                                                                            <  2:68976/65‑1 (MQ=255)                                                                                                                 aTTAGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAg                                                                                            >  1:68976/1‑65 (MQ=255)                                                                                                                    aGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCa                                                                                             >  2:846596/1‑61 (MQ=255)                                                                                                                    aGACGGAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCa                                                                                             <  1:846596/61‑1 (MQ=255)                                                                                                                        ggAACAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTg           <  1:602578/139‑1 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACt                         >  1:789874/1‑123 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACt                         <  2:789874/123‑1 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                >  2:1219197/1‑132 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTcagc                <  1:1219197/132‑1 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGtt         >  1:421084/1‑139 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGtt         >  1:404244/1‑139 (MQ=255)                                                                                                                          aaCAGTGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGtt         >  2:1345846/1‑139 (MQ=255)                                                                                                                               tGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                             <  1:574585/98‑1 (MQ=255)                                                                                                                               tGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGaa                                             >  2:574585/1‑98 (MQ=255)                                                                                                                               tGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGt    <  2:266695/139‑1 (MQ=255)                                                                                                                               tGCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGt    >  1:266695/1‑139 (MQ=255)                                                                                                                                gCCCCGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTa   >  1:1085592/1‑139 (MQ=255)                                                                                                                                 ccccGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAc  <  2:1085592/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        CACCCGGACAGTTAGAAGAGGCTACCCACCAGGCATTTTTATATACCGCACCATTAAAGACGACATACCAGGTCTGACCGCCCTGCTGTTTATTCCAGGCAACTACAGAAGAATTAGACGGAACAGTGCCCTGTGCTGGTGTAGCCGAACCGCCATTAGCCGGCGTATTGGATGCAGGCGTATTTGAATTTGAAGAAGATGAGCTGCCGCTCTTTTCGCAGGAAAGTGTATTGCCAAACTGACTAATTTCAGCAGCTGTTGCGGTAC  >  NC_000913/3469367‑3469633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑