Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,901,765 G→A G152G (GGC→GGT)  bglH ← carbohydrate‑specific outer membrane porin, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,901,7650GA98.6% 233.3 / NA 69G152G (GGC→GGT) bglHcarbohydrate‑specific outer membrane porin, cryptic
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (33/35);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAG  >  NC_000913/3901637‑3901898
                                                                                                                                |                                                                                                                                     
ccGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAAc                                                                                                                             >  2:1238524/1‑139 (MQ=255)
        gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc                                                                                                                     <  2:707893/139‑1 (MQ=255)
        gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc                                                                                                                     <  1:1238524/139‑1 (MQ=255)
        gTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc                                                                                                                     <  1:1401933/139‑1 (MQ=255)
           ctgctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCGAAGCGACCCAGAGAACCAATCGCCCAt                                                                                                                  >  2:867136/1‑139 (MQ=255)
              ctgACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGAt                                                                                                               >  1:485956/1‑139 (MQ=255)
                    aaCGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGt                                                                                                         <  2:1197476/139‑1 (MQ=255)
                     aCGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTg                                                                                                        <  2:222703/139‑1 (MQ=255)
                                catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGccc                                                                                                                    >  2:323423/1‑116 (MQ=255)
                                catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGccc                                                                                                                    <  1:323423/116‑1 (MQ=255)
                                catcatCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGagag                                                                                             >  1:1269846/1‑139 (MQ=255)
                                 atcatcACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCagag                                                                                                                                >  1:1171916/1‑103 (MQ=255)
                                 atcatcACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCagag                                                                                                                                <  2:1171916/103‑1 (MQ=255)
                                 atcatcACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAgg                                                                                            >  2:694969/1‑139 (MQ=255)
                                   catcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTg                                                                                                        <  1:713466/125‑1 (MQ=255)
                                   catcaCAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTg                                                                                                        >  2:713466/1‑125 (MQ=255)
                                        caACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc                                                                                     >  1:1382841/1‑139 (MQ=255)
                                        caACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccc                                                                                     >  2:1484410/1‑139 (MQ=255)
                                           cGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCa                                                                                  >  1:218877/1‑139 (MQ=255)
                                             gCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAc                                                                                <  2:960969/139‑1 (MQ=255)
                                              cATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc                                                                               <  2:107222/139‑1 (MQ=255)
                                              cATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAAcc                                                                               >  1:925777/1‑139 (MQ=255)
                                                tCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAg                                                                             >  1:1085692/1‑139 (MQ=255)
                                                  aaCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGaa                                                                           <  1:867136/139‑1 (MQ=255)
                                                   aCCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAAc                                                                          >  2:1302742/1‑139 (MQ=255)
                                                        tttGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTaa                                                                     >  1:1202606/1‑139 (MQ=255)
                                                           gCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAg                                                                  <  1:1204974/139‑1 (MQ=255)
                                                                      tGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACt                                                       <  1:1293531/139‑1 (MQ=255)
                                                                              cGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCaa                                               >  2:577179/1‑139 (MQ=255)
                                                                               gttgttTTAACTGCAAATCAAACCAGCCGGATTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAt                                              >  1:578205/1‑139 (MQ=255)
                                                                               gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACt                                                       >  2:1397702/1‑130 (MQ=255)
                                                                               gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACt                                                       <  1:1397702/130‑1 (MQ=255)
                                                                               gttgttTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAAt                                              >  2:146287/1‑139 (MQ=255)
                                                                                        aCTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCttt                                     <  2:808172/139‑1 (MQ=255)
                                                                                         cTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc                                                                                                                     <  2:544990/58‑1 (MQ=255)
                                                                                         cTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGcc                                                                                                                     >  1:544990/1‑58 (MQ=255)
                                                                                         cTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTc                                    >  1:1485955/1‑139 (MQ=255)
                                                                                          tGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTca                                   <  1:87021/139‑1 (MQ=255)
                                                                                            cAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAgg                                                                                                          >  1:35598/1‑66 (MQ=255)
                                                                                            cAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAgg                                                                                                          <  2:35598/66‑1 (MQ=255)
                                                                                               aTCAAACCAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAAt                              <  1:526112/139‑1 (MQ=255)
                                                                                                      cAGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGa                       >  2:255993/1‑139 (MQ=255)
                                                                                                       aGCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaa                      <  1:717320/139‑1 (MQ=255)
                                                                                                        gCCGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaaa                     <  2:1269846/139‑1 (MQ=255)
                                                                                                         ccGGAGTATTCGTTACCAAAGCGACCCAGAGAACCaa                                                                                                                          >  1:1028893/1‑37 (MQ=255)
                                                                                                         ccGGAGTATTCGTTACCAAAGCGACCCAGAGAACCaa                                                                                                                          <  2:1028893/37‑1 (MQ=255)
                                                                                                         ccGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccccc                                                                                   >  2:1502696/1‑76 (MQ=255)
                                                                                                         ccGGAGTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTccccc                                                                                   <  1:1502696/76‑1 (MQ=255)
                                                                                                              gTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAAc                                                        <  2:579209/98‑1 (MQ=255)
                                                                                                              gTATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAAc                                                        >  1:579209/1‑98 (MQ=255)
                                                                                                               tATTCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCa              <  1:255993/139‑1 (MQ=255)
                                                                                                                attatttACCACAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCACCCACAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATTTTTCACAAATTTACTGAAATCTTTCag             <  1:976554/134‑1 (MQ=255)
                                                                                                                  tCGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCagag           <  1:694969/139‑1 (MQ=255)
                                                                                                                  tCGTTACCAAAGCGACCCAAAGAACCAATCGCCCATGATTTAGCTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCagag           <  2:980858/139‑1 (MQ=255)
                                                                                                                   cGTTACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGt          <  1:146287/139‑1 (MQ=255)
                                                                                                                     ttACCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCa        <  2:1314588/139‑1 (MQ=255)
                                                                                                                       aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag                                                                                               <  2:532902/50‑1 (MQ=255)
                                                                                                                       aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag                                                                                               >  1:784350/1‑50 (MQ=255)
                                                                                                                       aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag                                                                                               >  1:532902/1‑50 (MQ=255)
                                                                                                                       aCCAAAGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATgag                                                                                               <  2:784350/50‑1 (MQ=255)
                                                                                                                         cAAAGCGGCCCAGACAACCAATCGCCCAGGATTTCGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTACAGCCAATTTCATCTTTCACTAATTTACTGAAATCTTTCAGCGTCATGga    >  2:976554/1‑139 (MQ=255)
                                                                                                                           aaGCGACCTAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaag  >  1:980858/1‑139 (MQ=255)
                                                                                                                           aaGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCa        >  2:148924/1‑133 (MQ=255)
                                                                                                                           aaGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCa        <  1:148924/133‑1 (MQ=255)
                                                                                                                           aaGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGgaag  >  1:1030600/1‑139 (MQ=255)
                                                                                                                            aGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGGGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCa              >  1:292469/1‑126 (MQ=255)
                                                                                                                            aGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaa                      <  1:869339/118‑1 (MQ=255)
                                                                                                                            aGCGACCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCa              <  2:292469/126‑1 (MQ=255)
                                                                                                                            aGCGACCCAGAGAACCAATCGCCCATGATATAGGTGAACCATGAGGGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGaa                      >  2:869339/1‑118 (MQ=255)
                                                                                                                                |                                                                                                                                     
CCGGTAGAGTACTGCTGACCAACGTTACCATCCATCATCACAACGGCATCAACCCGTTTGCCGTTTTCGTTGTAGACACGTTGTTTTAACTGCAAATCAAACCAGCCGGAGTATTCGTTACCAAAGCGGCCCAGAGAACCAATCGCCCATGATTTAGGTGAACCATGAGAGGCGGTCCCCCAACCAGAACGGTAATAGCCGTTGTAACTAAAGCCAATTTCATCTTTCACAAATTTACTGAAATCTTTCAGAGTCATGGAAG  >  NC_000913/3901637‑3901898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑