| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,225,294 | A→G | G489G (GGA→GGG) | metH → | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,225,294 | 0 | A | G | 100.0% | 205.5 / NA | 56 | G489G (GGA→GGG) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/25); total (31/25) | |||||||||||
TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG > NC_000913/4225161‑4225427 | tGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg < 1:1117180/137‑1 (MQ=255)tGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg > 2:1117180/1‑137 (MQ=255) ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCAc < 2:21620/139‑1 (MQ=255) aGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGaaa > 1:1347280/1‑139 (MQ=255) aGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGaaa > 1:1483764/1‑139 (MQ=255) ttGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCga > 2:1094790/1‑139 (MQ=255) tGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCgag > 2:778704/1‑139 (MQ=255) tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCg > 1:1233940/1‑139 (MQ=255) ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg > 2:622151/1‑139 (MQ=255) cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:778704/139‑1 (MQ=255) cGATAAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 2:439027/139‑1 (MQ=255) aTGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGt > 1:820561/1‑139 (MQ=255) gAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc < 2:820561/139‑1 (MQ=255) agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTACTGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 2:178994/1‑139 (MQ=255) agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 1:794431/1‑139 (MQ=255) cGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc > 1:1244073/1‑139 (MQ=255) gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 1:169853/139‑1 (MQ=255) aTGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGa < 2:1483764/139‑1 (MQ=255) cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAagag > 1:577479/1‑139 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 1:390538/1‑128 (MQ=255) tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 2:390538/128‑1 (MQ=255) tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTc > 2:1456370/1‑139 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg > 2:777542/1‑106 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg < 1:777542/106‑1 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc < 2:45941/111‑1 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc > 1:45941/1‑111 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt < 1:1335541/116‑1 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt > 2:1335541/1‑116 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:467785/1‑139 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 2:794431/139‑1 (MQ=255) cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 2:1237495/139‑1 (MQ=255) aaaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCag < 2:577479/139‑1 (MQ=255) tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc > 1:1335167/1‑118 (MQ=255) tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc < 2:1335167/118‑1 (MQ=255) tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatcatc > 1:1381566/1‑139 (MQ=255) gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt < 1:1193274/139‑1 (MQ=255) gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt > 2:1193274/1‑139 (MQ=255) tGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt > 2:721696/1‑76 (MQ=255) tGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt < 1:721696/76‑1 (MQ=255) gtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGATCCAAACATCTTCGCgg < 1:781382/139‑1 (MQ=255) tAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGt < 2:836813/139‑1 (MQ=255) tGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTcg > 1:210654/1‑132 (MQ=255) tGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTcg < 2:210654/132‑1 (MQ=255) ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt > 1:459223/1‑93 (MQ=255) ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt < 2:459223/93‑1 (MQ=255) ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAAc < 2:1233940/139‑1 (MQ=255) cTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACt > 1:1078998/1‑139 (MQ=255) ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt < 2:187512/53‑1 (MQ=255) ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt > 1:187512/1‑53 (MQ=255) ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTgg > 1:206001/1‑139 (MQ=255) tGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 1:1059880/1‑139 (MQ=255) tGACGAACAGGGGCAGGCCGATACTCGCCCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCACACCGAAGAGAGTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 2:301675/1‑139 (MQ=255) aCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa > 1:78073/1‑139 (MQ=255) cAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:897799/50‑1 (MQ=255) cAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTTACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAag < 2:1078998/139‑1 (MQ=255) cAGGGGCAGGCCGATACTCGCGCACGGAAAATAGAGATTTGCCGTCGGGc > 2:897799/1‑50 (MQ=255) | TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG > NC_000913/4225161‑4225427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |