Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,242,259	A→G	intergenic (‑5/+367)	xylE ← / ← malG	D‑xylose transporter/maltose transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,259	0	A	G	100.0%	257.3 / NA	69	intergenic (‑5/+367)	xylE/malG	D‑xylose transporter/maltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/34);  total (35/34)

ACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGACCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCA  >  NC_000913/4242127‑4242391                                                                                                                                     |                                                                                                                                     acaaAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGcc                                                                                                                                <  2:1149198/139‑1 (MQ=255)    aaGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATATTAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTa                                                                                                                             <  2:66195/139‑1 (MQ=255)            aTTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATAGATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTc                                                                                                                     >  2:906381/1‑139 (MQ=255)             ttGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  1:707214/139‑1 (MQ=255)             ttGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  1:906381/139‑1 (MQ=255)                     cTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    >  1:636079/1‑131 (MQ=255)                     cTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  2:636079/131‑1 (MQ=255)                       cAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          <  1:1408201/139‑1 (MQ=255)                       cAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          >  1:168635/1‑139 (MQ=255)                         aCAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCa                                                                                                        <  1:197023/139‑1 (MQ=255)                             tACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    >  1:1432179/1‑123 (MQ=255)                             tACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  2:1432179/123‑1 (MQ=255)                               ccGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAt                                                                                                  <  1:28999/139‑1 (MQ=255)                               ccGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAt                                                                                                  >  2:538124/1‑139 (MQ=255)                                  gAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGa                                                                                               >  1:1511693/1‑139 (MQ=255)                                       aaCGGCGGTGTCGTAGCTAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCa                                                                                                        <  2:6850/125‑1 (MQ=255)                                       aaCGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCa                                                                                                        >  1:6850/1‑125 (MQ=255)                                                           ataataAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGcc                                                                                                                                    >  1:772799/1‑77 (MQ=255)                                                           ataataAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          <  2:1337713/103‑1 (MQ=255)                                                           ataataAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          >  1:1337713/1‑103 (MQ=255)                                                           ataataAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCa                                                                                 >  2:828289/1‑128 (MQ=255)                                                           ataataAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCa                                                                                 <  1:828289/128‑1 (MQ=255)                                                              ataaACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCttt                                                                                                             <  2:1322680/97‑1 (MQ=255)                                                              ataaACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCttt                                                                                                             >  1:1322680/1‑97 (MQ=255)                                                                 aaCCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  1:341306/87‑1 (MQ=255)                                                                 aaCCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    >  2:341306/1‑87 (MQ=255)                                                                 aaCCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTa                                                                <  2:1511693/139‑1 (MQ=255)                                                                              aGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACaa                                                   >  1:410938/1‑139 (MQ=255)                                                                               gCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    >  1:375135/1‑73 (MQ=255)                                                                               gCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCt                                                                                                                    <  2:375135/73‑1 (MQ=255)                                                                               gCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAAt                                                  >  2:312263/1‑139 (MQ=255)                                                                                cGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          >  2:1117051/1‑82 (MQ=255)                                                                                cGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTAt                                                                                                          <  1:1117051/82‑1 (MQ=255)                                                                                cGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAt                                                                                                  >  2:1130044/1‑90 (MQ=255)                                                                                cGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAt                                                                                                  <  1:1130044/90‑1 (MQ=255)                                                                                 gACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGc                                                >  1:287418/1‑139 (MQ=255)                                                                                      aGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTa                                           >  1:446762/1‑139 (MQ=255)                                                                                           aTCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTaa                                      <  2:465775/139‑1 (MQ=255)                                                                                           aTCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTaa                                      >  1:154924/1‑139 (MQ=255)                                                                                           aTCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTaa                                      <  1:1029557/139‑1 (MQ=255)                                                                                           aTCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTaa                                      <  2:410938/139‑1 (MQ=255)                                                                                              gAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGc                                                                              <  2:637968/96‑1 (MQ=255)                                                                                              gAAAATATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGc                                                                              >  1:637968/1‑96 (MQ=255)                                                                                                aaaTATATAACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCt                                 <  2:168635/139‑1 (MQ=255)                                                                                                      ataACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACaa                                                   <  2:1387975/115‑1 (MQ=255)                                                                                                      ataACTGGAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACaa                                                   >  1:1387975/1‑115 (MQ=255)                                                                                                             gAATTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCtt                    >  2:74255/1‑139 (MQ=255)                                                                                                              aaTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCtgt                          <  1:699100/132‑1 (MQ=255)                                                                                                              aaTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCtgt                          >  2:699100/1‑132 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCa                                                                                                   <  2:955250/58‑1 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCa                                                                                                   >  1:955250/1‑58 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAAt                                                  <  1:559334/107‑1 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAAt                                                  >  2:559334/1‑107 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTaa                  <  2:154924/139‑1 (MQ=255)                                                                                                               aTTATACTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTaa                  <  2:446762/139‑1 (MQ=255)                                                                                                                   taCTGGGTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATtgt              <  1:538124/139‑1 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTa                                                                                                           <  2:901354/41‑1 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTa                                                                                                           >  1:901354/1‑41 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCa                                                                                                   >  2:188058/1‑49 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCa                                                                                                   <  1:188058/49‑1 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAtt                                                                                                 <  1:248272/51‑1 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCAtt                                                                                                 >  2:248272/1‑51 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAgg                                                                               <  2:1322170/69‑1 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAgg                                                                               >  1:1322170/1‑69 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATaa         >  2:519435/1‑139 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATaa         >  2:843982/1‑139 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATaa         >  1:1216497/1‑139 (MQ=255)                                                                                                                        ggTATTCATTCAGGCCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATaa         >  1:115383/1‑139 (MQ=255)                                                                                                                               attcaGGCCTGCCTTGGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTAAAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCa  >  2:1006796/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                     ACAAAGACGGTATTGAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGACCTGCCTTAGACCATTCTGATCTTTTATCAGTGCATTGATGTTGGAATTGTCAGGCAGTTAAGGCAATTATCATTTTTTACAATGCGTTTACGTAATGTCTTTTCTGTGATCTTAATTGTGATAATTATCCA  >  NC_000913/4242127‑4242391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑