Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,518	A→G	I618I (ATT→ATC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,518	0	A	G	100.0%	237.5 / NA	64	I618I (ATT→ATC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/32);  total (32/32)

CGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTC  >  NC_000913/4285383‑4285652                                                                                                                                        |                                                                                                                                       cGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGAtt                                                                                                                                     >  2:1063878/1‑139 (MQ=255)     aCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgc                                                                                                                                 <  1:36425/139‑1 (MQ=255)     aCCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgc                                                                                                                                 <  1:25987/139‑1 (MQ=255)           tCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGaa                                                                                                                           >  2:1122315/1‑139 (MQ=255)             gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  1:869537/139‑1 (MQ=255)             gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  1:1431466/139‑1 (MQ=255)             gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  2:450667/139‑1 (MQ=255)              cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATAc                                                                                                                        <  2:1037951/139‑1 (MQ=255)              cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATAc                                                                                                                        >  2:696367/1‑139 (MQ=255)              cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATAc                                                                                                                        >  2:606934/1‑139 (MQ=255)               aTCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACg                                                                                                                       <  2:318931/139‑1 (MQ=255)                       aTTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgcg                                                                                                                                <  1:304394/122‑1 (MQ=255)                       aTTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgcg                                                                                                                                >  2:304394/1‑122 (MQ=255)                             ggTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGc                                                                                                         >  1:269044/1‑139 (MQ=255)                             ggTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGc                                                                                                         <  1:606934/139‑1 (MQ=255)                             ggTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGc                                                                                                         <  1:1173691/139‑1 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  1:1482709/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    <  2:1029230/139‑1 (MQ=255)                                   gATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGc                                                                                                   <  1:800075/139‑1 (MQ=255)                                                     tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 <  1:736832/139‑1 (MQ=255)                                                     tGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGACGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 <  1:956116/139‑1 (MQ=255)                                                      gCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 >  2:1133458/1‑138 (MQ=255)                                                      gCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 <  1:1133458/138‑1 (MQ=255)                                                          ctctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTc                                                                            >  1:1132214/1‑139 (MQ=255)                                                          ctctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTc                                                                            <  1:283383/139‑1 (MQ=255)                                                             ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACg                                                                                                                       <  2:756210/93‑1 (MQ=255)                                                             ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACg                                                                                                                       >  1:756210/1‑93 (MQ=255)                                                             ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                         <  1:538172/139‑1 (MQ=255)                                                                 agcagcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTgca                                                                     >  2:675877/1‑139 (MQ=255)                                                                     gcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgcg                                                                                                                                >  1:616710/1‑76 (MQ=255)                                                                     gcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgcg                                                                                                                                <  2:616710/76‑1 (MQ=255)                                                                      cTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 >  1:970153/1‑122 (MQ=255)                                                                      cTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACg                                                                >  1:1127478/1‑139 (MQ=255)                                                                      cTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACg                                                                >  1:121769/1‑139 (MQ=255)                                                                      cTTCTCGACTACGCCAGGATCGGCAAGCATCGAGGTATCGCCCAGGCTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 <  2:970153/122‑1 (MQ=255)                                                                                   ccAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCa                                                   >  1:814810/1‑139 (MQ=255)                                                                                    cAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAg                                                  <  1:472558/139‑1 (MQ=255)                                                                                       gATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCgg                                               >  1:727258/1‑139 (MQ=255)                                                                                           ggCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCa                                           >  2:984380/1‑139 (MQ=255)                                                                                           ggCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCa                                           >  2:1114264/1‑139 (MQ=255)                                                                                           ggCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCa                                           >  1:1158305/1‑139 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         >  2:1107664/1‑60 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  1:1107664/60‑1 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         >  1:1204100/1‑60 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  2:1204100/60‑1 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCg                                                                               <  1:938459/102‑1 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCg                                                                               >  2:938459/1‑102 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCACATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 >  2:1512939/1‑100 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCACATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGt                                                                                 <  1:1512939/100‑1 (MQ=255)                                                                                                 cGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                     >  2:1449607/1‑139 (MQ=255)                                                                                                 cGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                     >  1:1395062/1‑139 (MQ=255)                                                                                                 cGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                     >  1:1427229/1‑139 (MQ=255)                                                                                                 cGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTAGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                     >  2:979544/1‑139 (MQ=255)                                                                                                           tCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAccc                           <  2:658449/139‑1 (MQ=255)                                                                                                                cAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCa                                                                                                                  >  1:431823/1‑47 (MQ=255)                                                                                                                cAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCa                                                                                                                  <  2:431823/47‑1 (MQ=255)                                                                                                                  ggTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCg                    <  2:1132214/139‑1 (MQ=255)                                                                                                                        tGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTc              >  1:294610/1‑139 (MQ=255)                                                                                                                            aTCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCg          <  1:15755/139‑1 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAg                                                                                      >  1:237168/1‑62 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAg                                                                                      <  2:237168/62‑1 (MQ=255)                                                                                                                               gcccgccGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTAc       <  2:1482709/139‑1 (MQ=255)                                                                                                                                cccgccGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACa      <  2:727258/139‑1 (MQ=255)                                                                                                                                    ccgcGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTc  >  2:610442/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       CGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTC  >  NC_000913/4285383‑4285652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑