| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,341,750 | G→A | intergenic (‑122/‑161) | melR ← / → melA | melibiose operon transcriptional regulator, autoregulator/alpha‑galactosidase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,341,750 | 0 | G | A | 98.3% | 198.8 / NA | 59 | intergenic (‑122/‑161) | melR/melA | melibiose operon transcriptional regulator, autoregulator/alpha‑galactosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (33/25); minor base T (0/1); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAG > NC_000913/4341625‑4341884 | tCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGc > 1:142261/1‑139 (MQ=255) tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:992406/1‑139 (MQ=255) tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:1511381/1‑139 (MQ=255) tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 1:151143/1‑139 (MQ=255) tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGGGAGGTAGGCTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCATAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:295218/1‑139 (MQ=255) gCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGt > 2:103491/1‑139 (MQ=255) gCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGt > 1:1405998/1‑139 (MQ=255) ctctTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAAt > 1:655977/1‑139 (MQ=255) tCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt < 2:151143/139‑1 (MQ=255) tGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTccc < 2:1198040/139‑1 (MQ=255) tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt < 2:1438870/129‑1 (MQ=255) tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt > 1:1438870/1‑129 (MQ=255) tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACt < 1:103491/139‑1 (MQ=255) tGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTAc > 2:749773/1‑139 (MQ=255) ggAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCTAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActg < 1:1086936/136‑1 (MQ=255) ggAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCTAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActg > 2:1086936/1‑136 (MQ=255) aGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTgctgct > 1:636157/1‑139 (MQ=255) ggaggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGc < 1:295218/139‑1 (MQ=255) gaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGa > 2:228800/1‑139 (MQ=255) aTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAt < 2:655977/139‑1 (MQ=255) aTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAt < 2:1425445/139‑1 (MQ=255) aCTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTg > 1:377873/1‑139 (MQ=255) cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACg > 2:1160132/1‑126 (MQ=255) cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACg < 1:1160132/126‑1 (MQ=255) cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGtt > 1:141472/1‑139 (MQ=255) aGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc < 2:1073474/104‑1 (MQ=255) aGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc > 1:1073474/1‑104 (MQ=255) gggAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg < 1:1511381/139‑1 (MQ=255) cGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAA‑‑CTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCa > 1:1375709/1‑139 (MQ=255) ttttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCaa > 1:600949/1‑139 (MQ=255) cccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGa > 2:357985/1‑139 (MQ=255) ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCttt < 2:1122514/56‑1 (MQ=255) ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCttt > 1:1122514/1‑56 (MQ=255) ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGcc > 1:1091150/1‑139 (MQ=255) cAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAA‑‑CTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt < 2:1375709/139‑1 (MQ=255) ggggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCA‑GATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCgg < 1:599504/131‑1 (MQ=255) ggggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCA‑GATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCgg > 2:599504/1‑131 (MQ=255) ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCa < 1:1037509/63‑1 (MQ=255) ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCa > 2:1037509/1‑63 (MQ=255) ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActgct > 2:756832/1‑82 (MQ=255) ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActgct < 1:756832/82‑1 (MQ=255) cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc > 1:1130010/1‑139 (MQ=255) cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc > 2:311804/1‑139 (MQ=255) cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTATGc > 1:298319/1‑139 (MQ=255) ggggACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGa < 2:1405998/139‑1 (MQ=255) ggggACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGa < 1:357985/139‑1 (MQ=255) ccGAGTATCATGAGTCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAGCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc < 2:298319/139‑1 (MQ=255) ccGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc < 2:1091150/139‑1 (MQ=255) tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAt > 1:355731/1‑37 (MQ=255) tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAt < 2:355731/37‑1 (MQ=255) tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc < 2:1391923/49‑1 (MQ=255) tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc > 1:1391923/1‑49 (MQ=255) aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCa < 1:438594/69‑1 (MQ=255) aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCa > 2:438594/1‑69 (MQ=255) aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGaa > 1:285789/1‑139 (MQ=255) aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGaa < 2:600949/139‑1 (MQ=255) tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCt > 2:280318/1‑74 (MQ=255) tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCt < 1:280318/74‑1 (MQ=255) tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAg < 2:142261/139‑1 (MQ=255) | TCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAG > NC_000913/4341625‑4341884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |