Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	631,934	A→G	T681A (ACG→GCG)	cstA →	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	631,934	0	A	G	100.0%	243.0 / NA	65	T681A (ACG→GCG)	cstA	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/31);  total (34/31)

GTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC  >  NC_000913/631808‑632066                                                                                                                               |                                                                                                                                     gtgtTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAAc                                                                                                                          <  2:1412576/139‑1 (MQ=255) gtgtTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAAc                                                                                                                          <  1:166878/139‑1 (MQ=255)   gtTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCa                                                                                                                        >  2:1391268/1‑139 (MQ=255)   gtTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCa                                                                                                                        <  2:306401/139‑1 (MQ=255)           acCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTTGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCTGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGa                                                                                                                >  1:1122744/1‑139 (MQ=255)              gTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGaaaa                                                                                                             >  2:1499148/1‑139 (MQ=255)                 tGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGt                                                                                                          >  1:44252/1‑139 (MQ=255)                   gATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCg                                                                                                        >  1:1326047/1‑139 (MQ=255)                       ccGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCgagga                                                                                                    >  1:1110313/1‑139 (MQ=255)                             tAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGt                                                                                              >  2:1280543/1‑139 (MQ=255)                                 cATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGgcg                                                                                          <  1:1470337/139‑1 (MQ=255)                                         ttATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCaaa                                                                                  >  2:1143078/1‑139 (MQ=255)                                             ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGa                                                                                                                >  2:714499/1‑105 (MQ=255)                                             ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGa                                                                                                                <  1:714499/105‑1 (MQ=255)                                             ggtggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgc                                                                              <  1:1475244/139‑1 (MQ=255)                                               tggtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcgc                                                                            <  2:44252/139‑1 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGcc                                                                                                                   >  1:1489712/1‑98 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGcc                                                                                                                   <  2:1489712/98‑1 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  2:112460/1‑139 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  1:119510/1‑139 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  2:33086/1‑139 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  2:386467/1‑139 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  1:216591/1‑139 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          <  2:1318728/139‑1 (MQ=255)                                                 gtCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGcac                                                                          >  1:1318728/1‑139 (MQ=255)                                                  tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcgc                                                                            >  2:1226181/1‑136 (MQ=255)                                                  tCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcgc                                                                            <  1:1226181/136‑1 (MQ=255)                                                    gtggtgGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACt                                                                       <  1:1280543/139‑1 (MQ=255)                                                         ggtTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGt                                                                  <  2:359772/139‑1 (MQ=255)                                                                gCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCg                                                                                                                                    >  1:1344928/1‑66 (MQ=255)                                                                     gTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAgggg                                                      >  1:1195177/1‑139 (MQ=255)                                                                      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCag                                                               <  2:1472673/129‑1 (MQ=255)                                                                      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCag                                                               >  1:1472673/1‑129 (MQ=255)                                                                      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggg                                                     <  2:385495/139‑1 (MQ=255)                                                                      ttCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAggggg                                                     >  2:768973/1‑139 (MQ=255)                                                                                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATg                                                                                                           >  2:36264/1‑75 (MQ=255)                                                                                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATg                                                                                                           <  1:36264/75‑1 (MQ=255)                                                                                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcgc                                                                            <  1:137992/106‑1 (MQ=255)                                                                                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcgc                                                                            >  2:137992/1‑106 (MQ=255)                                                                                aGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGc                                           >  1:705322/1‑139 (MQ=255)                                                                                      cACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcg                                                                             >  2:338153/1‑99 (MQ=255)                                                                                      cACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcg                                                                             <  1:338153/99‑1 (MQ=255)                                                                                        cTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTg                                   <  1:768973/139‑1 (MQ=255)                                                                                         ttGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcg                                                                             <  2:408634/96‑1 (MQ=255)                                                                                         ttGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGgcg                                                                             >  1:408634/1‑96 (MQ=255)                                                                                           gCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCt                                <  1:386467/139‑1 (MQ=255)                                                                                             ggCATTGAAAGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTa                              <  1:1315607/139‑1 (MQ=255)                                                                                                    aaaGATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                       <  1:1499148/139‑1 (MQ=255)                                                                                                       gATCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAggg                    <  1:33086/139‑1 (MQ=255)                                                                                                        aTCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                   <  2:119510/139‑1 (MQ=255)                                                                                                         tCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAAt                                       <  1:1076117/118‑1 (MQ=255)                                                                                                         tCCGAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAAt                                       >  2:1076117/1‑118 (MQ=255)                                                                                                            gAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTa                              <  2:138667/124‑1 (MQ=255)                                                                                                            gAAGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTa                              >  1:138667/1‑124 (MQ=255)                                                                                                              aGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc             >  1:897471/1‑139 (MQ=255)                                                                                                              aGCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctc             >  1:1139495/1‑139 (MQ=255)                                                                                                               gCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTgg                                            <  2:1372893/107‑1 (MQ=255)                                                                                                               gCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTgg                                            >  1:1372893/1‑107 (MQ=255)                                                                                                               gCCAACGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCctct            <  2:1122744/139‑1 (MQ=255)                                                                                                                  aaCGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAAt                                                                                                            >  1:158585/1‑40 (MQ=255)                                                                                                                  aaCGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAAt                                                                                                            <  2:158585/40‑1 (MQ=255)                                                                                                                    cGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                       >  1:560495/1‑123 (MQ=255)                                                                                                                    cGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCa                       <  2:560495/123‑1 (MQ=255)                                                                                                                    cGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTAGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                   >  1:682848/1‑127 (MQ=255)                                                                                                                    cGGCGAAAGAAGCGCCGTATGAACCAATGCCGGAAAATGTAGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGt                   <  2:682848/127‑1 (MQ=255)                                                                                                                        gaaagaagCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAc   <  2:1139495/139‑1 (MQ=255)                                                                                                                         aaagaagCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAAcc  <  1:428975/139‑1 (MQ=255)                                                                                                                          aagaagCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTg                                                           <  1:748895/81‑1 (MQ=255)                                                                                                                          aagaagCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTg                                                           >  2:748895/1‑81 (MQ=255)                                                                                                                               |                                                                                                                                     GTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACC  >  NC_000913/631808‑632066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑