Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,910	G→A	V203M (GTG→ATG)	fimC →	periplasmic chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,910	0	G	A	100.0%	255.0 / NA	73	V203M (GTG→ATG)	fimC	periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/38);  total (35/38)

TCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAA  >  NC_000913/4544786‑4545040                                                                                                                             |                                                                                                                                   tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGc                                                                                                                      >  1:1160306/1‑139 (MQ=255) tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGc                                                                                                                      <  2:1074010/139‑1 (MQ=255)        gCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                                               <  2:548731/139‑1 (MQ=255)          aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACg                                                                                                             >  1:720828/1‑139 (MQ=255)                                  cgcgAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     >  1:1378588/1‑139 (MQ=255)                                  cgcgAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     <  2:720828/139‑1 (MQ=255)                                  cgcgAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     <  1:184395/139‑1 (MQ=255)                                      aaTTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 <  1:71891/139‑1 (MQ=255)                                                     aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  <  2:251059/139‑1 (MQ=255)                                                     aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  <  1:1450717/139‑1 (MQ=255)                                                     aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  <  2:1195720/139‑1 (MQ=255)                                                          cccGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATa                                                             <  2:463466/139‑1 (MQ=255)                                                           ccGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATaa                                                            >  2:235553/1‑139 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     >  2:541270/1‑110 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     <  1:541270/110‑1 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   <  1:691660/112‑1 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   >  2:691660/1‑112 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  >  1:854827/1‑129 (MQ=255)                                                               cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  <  2:854827/129‑1 (MQ=255)                                                                    tATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGTCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATg                                                   <  2:922107/139‑1 (MQ=255)                                                                     aTTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGtt                                                                                                          <  2:588631/83‑1 (MQ=255)                                                                     aTTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGtt                                                                                                          >  1:588631/1‑83 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAGAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTg                                                                                                    <  1:483409/82‑1 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAGAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTg                                                                                                    >  2:483409/1‑82 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                                  >  2:147093/1‑84 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                                  <  1:147093/84‑1 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     >  2:1340800/1‑97 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     <  2:1255264/97‑1 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     <  1:1340800/97‑1 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                                     >  1:1255264/1‑97 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 <  2:424082/101‑1 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 >  1:424082/1‑101 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                                           >  1:1007652/1‑139 (MQ=255)                                                                            gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                                           >  2:854976/1‑139 (MQ=255)                                                                             aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTa                                          >  1:1415935/1‑139 (MQ=255)                                                                              cGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAc                                         >  2:771680/1‑139 (MQ=255)                                                                              cGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAc                                         >  2:1175896/1‑139 (MQ=255)                                                                                gTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAccc                                       >  1:838562/1‑139 (MQ=255)                                                                                   aCAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   >  1:33975/1‑92 (MQ=255)                                                                                   aCAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   <  2:33975/92‑1 (MQ=255)                                                                                    cAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  >  1:754249/1‑108 (MQ=255)                                                                                    cAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                                  <  2:754249/108‑1 (MQ=255)                                                                                       agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 >  1:464801/1‑90 (MQ=255)                                                                                       agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 <  2:464801/90‑1 (MQ=255)                                                                                         ttGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGa                              <  2:1419284/139‑1 (MQ=255)                                                                                          tGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGAc                             <  1:235553/139‑1 (MQ=255)                                                                                           gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 >  2:525165/1‑86 (MQ=255)                                                                                           gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 <  1:525165/86‑1 (MQ=255)                                                                                             aTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg                          <  1:959067/139‑1 (MQ=255)                                                                                             aTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg                          <  2:1378588/139‑1 (MQ=255)                                                                                             aTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg                          <  1:622567/139‑1 (MQ=255)                                                                                             aTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAACCCCAAAATGACggg                          <  2:1007652/139‑1 (MQ=255)                                                                                              tGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                                        <  2:947696/76‑1 (MQ=255)                                                                                              tGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                                        >  1:947696/1‑76 (MQ=255)                                                                                               gCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACt                                            >  1:915172/1‑119 (MQ=255)                                                                                               gCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACt                                            <  2:915172/119‑1 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGTGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                                        <  2:896894/73‑1 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGtt                                                                                                          >  1:684582/1‑55 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGtt                                                                                                          <  2:684582/55‑1 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                                  >  2:956206/1‑63 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                                  <  1:956206/63‑1 (MQ=255)                                                                                                 cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCGTTCTGATGCa                                                                                        >  1:896894/1‑73 (MQ=255)                                                                                                   gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                                    <  2:1050474/91‑1 (MQ=255)                                                                                                   gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                                    >  1:1050474/1‑91 (MQ=255)                                                                                                      cccGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGAt                                                       <  2:784023/101‑1 (MQ=255)                                                                                                      cccGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGAt                                                       >  1:784023/1‑101 (MQ=255)                                                                                                      cccGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGa                 >  1:265996/1‑139 (MQ=255)                                                                                                          ggTTCTTGAAAATGCGTTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 <  2:1320110/71‑1 (MQ=255)                                                                                                          ggTTCTTGAAAATGCGTTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                                                 >  1:1320110/1‑71 (MQ=255)                                                                                                            atCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGCTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTCACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACCGGCGTAATGGAATAACg           >  1:928496/2‑139 (MQ=255)                                                                                                                     aTGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   >  1:908228/1‑58 (MQ=255)                                                                                                                     aTGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                                   <  2:908228/58‑1 (MQ=255)                                                                                                                     aTGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGaa  >  2:274718/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                   TCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAATGGAATAACGCAGGGGGAA  >  NC_000913/4544786‑4545040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑