Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,972,909	(C)6→7	coding (954/1194 nt)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,972,903	1	.	C	100.0%	104.3 / NA	28	G320G (GGC→GGG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (13/15);  total (13/15)

TGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑CCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAG  >  NC_000913/2972782‑2973028                                                                                                                           |                                                                                                                              tGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCaa                                                                                                               <  1:35244/139‑1 (MQ=255)   cccAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATc                                                                                                             <  2:480389/139‑1 (MQ=255)      aGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGc                                                                                                          >  1:335945/1‑139 (MQ=255)      aGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑cc                                                                                                                             >  2:411634/1‑119 (MQ=255)      aGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑cc                                                                                                                             <  1:411634/119‑1 (MQ=255)             acaacaTGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATcaaca                                                                                                   >  1:204234/1‑139 (MQ=255)                   tGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCAt                                                                                             <  2:335945/139‑1 (MQ=255)                    ggCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATa                                                                                            >  1:307056/1‑139 (MQ=255)                      cgcTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCAt                                                                                             <  2:396674/136‑1 (MQ=255)                      cgcTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCAt                                                                                             >  1:396674/1‑136 (MQ=255)                        cTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCt                                                                                        <  2:109331/139‑1 (MQ=255)                            tttCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGgca                                                                                    <  2:307056/139‑1 (MQ=255)                                                              cgccccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTaccac                                                  >  1:322916/1‑139 (MQ=255)                                                                ccccgACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTAccacca                                                <  2:322916/139‑1 (MQ=255)                                                                                aCCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATccc                                <  1:509601/139‑1 (MQ=255)                                                                                  cccGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCgg                              <  2:204234/139‑1 (MQ=255)                                                                                     gCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTg                           >  1:27437/1‑139 (MQ=255)                                                                                      cTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACg                                              <  1:333441/119‑1 (MQ=255)                                                                                      cTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACg                                              >  2:333441/1‑119 (MQ=255)                                                                                                 aCGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCaa                                                                                                               >  1:278096/1‑43 (MQ=255)                                                                                                 aCGCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCaa                                                                                                               <  2:278096/43‑1 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCt                            <  1:249698/124‑1 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCt                            >  2:249698/1‑124 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTg               <  1:455651/137‑1 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTg               >  2:455651/1‑137 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGG‑AAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGaca            <  1:446935/139‑1 (MQ=255)                                                                                                   gCATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGG‑AAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGaca            >  2:446935/1‑139 (MQ=255)                                                                                                    cATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGc                         <  2:591698/126‑1 (MQ=255)                                                                                                    cATTGAGCGGAACGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGc                         >  1:591698/1‑126 (MQ=255)                                                                                                              aaCGACAAAAAAGCCCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAg  >  1:512522/1‑139 (MQ=255)                                                                                                                           |                                                                                                                              TGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAG‑CCCCCCATCACGCCAATCAGCATCAACAAGGCATAGGCTGGCAGCAGCTCGTGTTGCAGGGAAAAAATCAGTACCACCACGCCAATCAAAATCCCGGCTGGCATACAGCGTGACACGGTTTCCAG  >  NC_000913/2972782‑2973028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑