Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	219,650	G→A	P116S (CCA→TCA)	rcsF ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	219,650	0	G	A	100.0%	129.7 / NA	38	P116S (CCA→TCA)	rcsF	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

ACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGT  >  NC_000913/219514‑219781                                                                                                                                         |                                                                                                                                    aCCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACg                                                                                                                                   <  2:533362/139‑1 (MQ=255)      gctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCg                                                                                                                              <  2:251497/139‑1 (MQ=255)           cgcgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTGCCGCTGGt                                                                                                                         >  1:296757/1‑139 (MQ=255)           cgcgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGt                                                                                                                         >  2:397861/1‑139 (MQ=255)           cgcgGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGt                                                                                                                         >  1:566756/1‑139 (MQ=255)                     cGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAg                                                                                                               <  1:397861/139‑1 (MQ=255)                        aCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTa                                                                                                            <  1:85686/139‑1 (MQ=255)                                 tataGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCg                                                                                                                  <  1:103979/124‑1 (MQ=255)                                 tataGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCg                                                                                                                  >  2:103979/1‑124 (MQ=255)                                    aGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCTCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACa                                                                                                >  1:107927/1‑139 (MQ=255)                                              aaTAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCtt                                                                                      <  2:296757/139‑1 (MQ=255)                                                        aTTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAg                                                                                                       >  1:203299/1‑112 (MQ=255)                                                        aTTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAg                                                                                                       <  2:203299/112‑1 (MQ=255)                                                         tttGCTCGAACTGGAAACTGCTCATTTCGACGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCCGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTagag                                                                           >  2:407958/1‑139 (MQ=255)                                                              tCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTa                                                                                                                                 <  1:348240/80‑1 (MQ=255)                                                              tCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTa                                                                                                                                 >  2:348240/1‑80 (MQ=255)                                                                            gCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACTGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGAGTTGATTTGCATCCGCt                                                        <  1:350072/139‑1 (MQ=255)                                                                             cTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACtt                                                                                                                    >  2:227569/1‑78 (MQ=255)                                                                             cTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACtt                                                                                                                    <  1:227569/78‑1 (MQ=255)                                                                                               tAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCt                                     <  1:294353/139‑1 (MQ=255)                                                                                                 aGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGTTcg                                   >  1:238034/1‑139 (MQ=255)                                                                                                      aGAACCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                              >  1:16408/1‑139 (MQ=255)                                                                                                        aaCCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgaga                            >  2:462094/1‑139 (MQ=255)                                                                                                        aaCCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgaga                            >  1:438933/1‑139 (MQ=255)                                                                                                        aaCCGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGgaga                            <  2:107927/139‑1 (MQ=255)                                                                                                          ccGATACATACAGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGt                          >  2:69978/1‑139 (MQ=255)                                                                                                                  tacaGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTa                  <  1:558767/139‑1 (MQ=255)                                                                                                                     aGCCTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTagag               >  2:163527/1‑139 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCg                                                                                                                  <  2:376452/38‑1 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCg                                                                                                                  >  1:376452/1‑38 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATg                                                                                                          <  2:339659/46‑1 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATg                                                                                                          >  1:339659/1‑46 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                              >  2:401019/1‑122 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                              <  1:401019/122‑1 (MQ=255)                                                                                                                       ccTGACGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGc             <  2:566756/139‑1 (MQ=255)                                                                                                                           aCGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                              >  1:467028/1‑118 (MQ=255)                                                                                                                           aCGATAGCAGCCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga                              <  2:467028/118‑1 (MQ=255)                                                                                                                                  cagcCTGACGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGTCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTACAGGCCTGGCAAGAGt  <  1:407958/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    ACCTGGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGT  >  NC_000913/219514‑219781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑