Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,065,209	C→T	P392S (CCT→TCT)	yccE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,065,209	0	C	T	100.0%	158.5 / NA	45	P392S (CCT→TCT)	yccE	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/19);  total (26/19)

TTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTA  >  NC_000913/1065086‑1065342                                                                                                                            |                                                                                                                                      ttatttatTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTc                                                                                                                        >  1:141378/1‑139 (MQ=255)            tAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTc                                                                                                                        >  2:350504/1‑128 (MQ=255)            tAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTc                                                                                                                        <  1:350504/128‑1 (MQ=255)              aTAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGtt                                                                                                           >  2:204218/1‑139 (MQ=255)              aTAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGtt                                                                                                           >  1:482362/1‑139 (MQ=255)                          cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTAc                                                                                                                <  2:274717/122‑1 (MQ=255)                          cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTAc                                                                                                                >  1:274717/1‑122 (MQ=255)                          cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATa                                                                                                   <  1:105968/135‑1 (MQ=255)                          cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATa                                                                                                   >  2:105968/1‑135 (MQ=255)                          cATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGa                                                                                               >  1:587624/1‑139 (MQ=255)                                    cTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATgcg                                                                                     <  2:171487/139‑1 (MQ=255)                                       ggggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTAc                                                                                                                >  2:527683/1‑109 (MQ=255)                                       ggggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTAc                                                                                                                <  1:527683/109‑1 (MQ=255)                                       ggggAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCaa                                                                                  >  2:213892/1‑139 (MQ=255)                                                      ttCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAAt                                                                   <  2:482362/139‑1 (MQ=255)                                                             gATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             >  1:226138/1‑122 (MQ=255)                                                             gATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             <  2:226138/122‑1 (MQ=255)                                                                      cTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAAt                                                   >  1:298762/1‑139 (MQ=255)                                                                        tGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             <  2:61617/111‑1 (MQ=255)                                                                        tGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             >  1:61617/1‑111 (MQ=255)                                                                        tGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATgg                                                 >  2:490867/1‑139 (MQ=255)                                                                        tGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATgg                                                 >  2:487125/1‑139 (MQ=255)                                                                          aGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCa                                               <  1:204218/139‑1 (MQ=255)                                                                              tGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAgg                                           >  1:151837/1‑139 (MQ=255)                                                                               ggTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTa                                                                                                          >  2:433993/1‑75 (MQ=255)                                                                               ggTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTa                                                                                                          <  1:433993/75‑1 (MQ=255)                                                                                 ttATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCat                                        >  1:14651/1‑139 (MQ=255)                                                                                  tATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCata                                       <  1:61796/139‑1 (MQ=255)                                                                                    tttAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACa                                                                                                      >  1:482415/1‑74 (MQ=255)                                                                                    tttAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACa                                                                                                      <  2:482415/74‑1 (MQ=255)                                                                                      tAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             <  2:150583/97‑1 (MQ=255)                                                                                      tAATGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             >  1:150583/1‑97 (MQ=255)                                                                                         tGAACACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTaaa                                <  1:213892/139‑1 (MQ=255)                                                                                           aaCACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTc                                                                                                                        >  1:410895/1‑49 (MQ=255)                                                                                           aaCACTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTc                                                                                                                        <  2:410895/49‑1 (MQ=255)                                                                                             cacTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAcc                            <  2:137887/139‑1 (MQ=255)                                                                                             cacTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAAcc                            <  2:141378/139‑1 (MQ=255)                                                                                               cTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCa                           >  2:459034/1‑138 (MQ=255)                                                                                               cTGGCAATCATTATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCa                           <  1:459034/138‑1 (MQ=255)                                                                                                          tATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             >  1:74716/1‑77 (MQ=255)                                                                                                          tATGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGa                                                                             <  2:74716/77‑1 (MQ=255)                                                                                                            tGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtctc             >  2:436049/1‑139 (MQ=255)                                                                                                            tGACGTTTACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGtctc             >  2:219157/1‑139 (MQ=255)                                                                                                                   tACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAGAAACCATTGTTATTAGTCTCACACttt      >  1:159538/1‑139 (MQ=255)                                                                                                                   tACCTCCCTTCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACttt      >  2:305317/1‑139 (MQ=255)                                                                                                                       tcccttcTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTa  <  2:14651/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                      TTATTTATTGTTAATAGTAATGTGGCATCAATCTCCTTGGGGAATGAATCCTTTTCAACAGATGAAGATCTTGAGTATGGTTATTTAATGAACACTGGCAATCATTATGACGTTTACCTCCCTCCTGAACTTTTTGCTCAGGCTTACAAGTTAAACAATAAGGAAATGAATGCGCAACTCGACTATTTAAATCGTTATGCAATTTAATGGCAAAGGCATATGCTAAAAACCATTGTTATTAGTCTCACACTTTTTTA  >  NC_000913/1065086‑1065342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑