| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,464 | T→C | V256A (GTT→GCT) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,464 | 0 | T | C | 100.0% | 96.9 / NA | 26 | V256A (GTT→GCT) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/13); total (13/13) | |||||||||||
GCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG > NC_000913/1756332‑1756598 | gcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt < 1:103418/139‑1 (MQ=255) cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAATTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTc > 1:587417/1‑139 (MQ=255) aaaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc < 2:223160/139‑1 (MQ=255) aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc < 1:268453/139‑1 (MQ=255) cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAATTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCag < 2:587417/139‑1 (MQ=255) tctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt > 2:518132/1‑121 (MQ=255) tctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt < 1:518132/121‑1 (MQ=255) ggAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCa > 2:160186/1‑139 (MQ=255) tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa > 1:418820/1‑128 (MQ=255) tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa < 2:418820/128‑1 (MQ=255) tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGAt > 2:70212/1‑139 (MQ=255) tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg > 1:274749/1‑119 (MQ=255) tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg < 2:274749/119‑1 (MQ=255) atcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGt > 2:159230/1‑90 (MQ=255) atcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGt < 1:159230/90‑1 (MQ=255) catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc < 1:6417/58‑1 (MQ=255) catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc > 2:6417/1‑58 (MQ=255) ttGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc < 1:535459/53‑1 (MQ=255) ttGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc > 2:535459/1‑53 (MQ=255) cgcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACg > 2:536372/1‑139 (MQ=255) gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg > 2:420226/1‑78 (MQ=255) gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg < 1:420226/78‑1 (MQ=255) acccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa > 2:375400/2‑139 (MQ=255) ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt > 2:11623/1‑50 (MQ=255) ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt < 1:11623/50‑1 (MQ=255) gaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg < 1:70212/139‑1 (MQ=255) | GCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG > NC_000913/1756332‑1756598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |