Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,702	T→C	A10A (GCA→GCG)	nlpC ←	putative C40 clan peptidase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,702	0	T	C	100.0%	111.2 / NA	30	A10A (GCA→GCG)	nlpC	putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

CGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAT  >  NC_000913/1792588‑1792829                                                                                                                   |                                                                                                                                cGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGaaa                                                                                                         >  2:124429/1‑139 (MQ=255)                   tAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTcc                                                                                       <  1:591110/139‑1 (MQ=255)                    aGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCct                                                                                      <  1:124429/139‑1 (MQ=255)                    aGCCGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCct                                                                                      <  2:393503/139‑1 (MQ=255)                         gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCa                                                                                                             >  1:448684/1‑111 (MQ=255)                         gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCa                                                                                                             <  2:448684/111‑1 (MQ=255)                         gTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGtt                                                                                 >  1:577008/1‑139 (MQ=255)                                                            aaaGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc                                              <  2:456941/139‑1 (MQ=255)                                                                 cTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGAtt                                         <  2:519707/139‑1 (MQ=255)                                                                              gcggTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt                                                                        >  2:519609/1‑95 (MQ=255)                                                                              gcggTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt                                                                        <  1:519609/95‑1 (MQ=255)                                                                                         tggtggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAAc                                                                                                        <  1:607825/52‑1 (MQ=255)                                                                                         tggtggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAAc                                                                                                        >  2:607825/1‑52 (MQ=255)                                                                                            tggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCa                                                                                                     >  1:532759/1‑52 (MQ=255)                                                                                            tggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCa                                                                                                     <  2:532759/52‑1 (MQ=255)                                                                                             ggCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTc             <  2:577008/139‑1 (MQ=255)                                                                                              gCTACACCCGGCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCg            >  2:185525/1‑139 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTc                                                                                        <  1:542834/53‑1 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTc                                                                                        >  2:542834/1‑53 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCctc                                                                                     >  1:53804/1‑56 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCctc                                                                                     <  2:53804/56‑1 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt                                                                        >  1:560384/1‑69 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCt                                                                        <  2:560384/69‑1 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc                                              <  2:303745/95‑1 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTAcc                                              >  1:303745/1‑95 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGc    >  2:201249/1‑137 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGc    <  1:201249/137‑1 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAt  >  2:146839/1‑139 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATC‑AAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGcg                                                         >  2:529799/1‑83 (MQ=255)                                                                                                        gCCAGAAGCAGCGCTGTGATC‑AAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGcg                                                         <  1:529799/83‑1 (MQ=255)                                                                                                                   |                                                                                                                                CGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAT  >  NC_000913/1792588‑1792829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑