Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,907,417	G→A	Q7* (CAG→TAG)	cspC ←	stress protein, member of the CspA‑family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,907,417	0	G	A	100.0%	73.9 / NA	23	Q7* (CAG→TAG)	cspC	stress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/11);  total (12/11)

AACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACG  >  NC_000913/1907286‑1907547                                                                                                                                    |                                                                                                                                   aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTa                                                                                                                             >  1:140515/1‑139 (MQ=255)                      agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCAt                                                                                                                  <  2:48634/129‑1 (MQ=255)                      agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCAt                                                                                                                  >  1:48634/1‑129 (MQ=255)                                ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg                                                                                              <  1:549443/139‑1 (MQ=255)                                                cGGTGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAAGCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                              >  1:392846/1‑139 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGaaaa                                                                                                           >  1:38652/1‑110 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGaaaa                                                                                                           <  2:38652/110‑1 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                              >  2:584325/1‑139 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                              >  1:405759/1‑139 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                              >  2:471184/1‑139 (MQ=255)                                                cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAg                                                                              >  2:177906/1‑139 (MQ=255)                                                          tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCa                                                                           >  1:252104/1‑132 (MQ=255)                                                          tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCa                                                                           <  2:252104/132‑1 (MQ=255)                                                             gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGAt                                                                 <  2:297964/139‑1 (MQ=255)                                                                  caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACa                                                                      >  1:154774/1‑129 (MQ=255)                                                                  caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACa                                                                      <  2:154774/129‑1 (MQ=255)                                                                  caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAAc                                                            >  1:469003/1‑139 (MQ=255)                                                                    tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTg                                                          <  2:392846/139‑1 (MQ=255)                                                                         gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGAcaca                                                     <  2:469003/139‑1 (MQ=255)                                                                                      ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAgg                                        <  1:584325/139‑1 (MQ=255)                                                                                        aGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCa                                      <  2:420181/139‑1 (MQ=255)                                                                                         gTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTAACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCAc                                     <  2:479298/139‑1 (MQ=255)                                                                                                                            cTTAACCTAACCTTTAATCTTTGCCATTGGAAAAGTTCCTTAGATTGTTCTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTAGGCAGAGAAGCGGTATtcaacg  >  1:395147/1‑139 (MQ=255)                                                                                                                              taacctaaccTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCt                         <  1:599379/114‑1 (MQ=255)                                                                                                                              taacctaaccTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCt                         >  2:599379/1‑114 (MQ=255)                                                                                                                                    |                                                                                                                                   AACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCGCAGGCATAACATAGATAAAACTGACACATTACTGCATGAGGCACCAATATAAGGCTCGGCAGAGAAGCGGTATTCAACG  >  NC_000913/1907286‑1907547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑