Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,968,408 C→T V32M (GTG→ATG)  cheB ← fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,968,4080CT100.0% 75.8 / NA 23V32M (GTG→ATG) cheBfused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/14);  total (9/14)

GCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACA  >  NC_000913/1968279‑1968526
                                                                                                                                 |                                                                                                                      
gCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCAt                                                                                                               >  2:420464/1‑139 (MQ=255)
                ttCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtg                                                                                               >  1:13853/1‑139 (MQ=255)
                  cGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtgat                                                                                             <  1:130279/139‑1 (MQ=255)
                  cGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTtgat                                                                                             <  1:284781/139‑1 (MQ=255)
                       aaaTCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt                                                                                        <  2:43828/139‑1 (MQ=255)
                        aaTCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTc                                                                                       <  2:89941/139‑1 (MQ=255)
                                                        tCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAatcat                                                       <  1:268180/139‑1 (MQ=255)
                                                          cAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCg                                                     <  2:13853/139‑1 (MQ=255)
                                                           aGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGa                                                    >  2:384515/1‑139 (MQ=255)
                                                               tCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATc                                                                             >  1:116076/1‑110 (MQ=255)
                                                               tCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATc                                                                             <  2:116076/110‑1 (MQ=255)
                                                                                aaTTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt                                                                                        >  1:584171/1‑82 (MQ=255)
                                                                                aaTTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGAttt                                                                                        <  2:584171/82‑1 (MQ=255)
                                                                                        aaTCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt                       <  2:561659/139‑1 (MQ=255)
                                                                                             aGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATcc                  <  2:495015/139‑1 (MQ=255)
                                                                                               tCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt                       <  2:74019/132‑1 (MQ=255)
                                                                                               tCACGCGCGACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGtt                       >  1:74019/1‑132 (MQ=255)
                                                                                                   gcgcgACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCcttactt            <  1:420464/139‑1 (MQ=255)
                                                                                                    cgcgACCAGCGGATCAGGCGCGGTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCcttactta           >  1:564887/1‑139 (MQ=255)
                                                                                                                          gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGa                                                                               >  2:320116/1‑49 (MQ=255)
                                                                                                                          gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGa                                                                               <  1:320116/49‑1 (MQ=255)
                                                                                                                          gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATaca  <  1:214787/126‑1 (MQ=255)
                                                                                                                          gTCGCCATCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATaca  >  2:214787/1‑126 (MQ=255)
                                                                                                                                 |                                                                                                                      
GCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGATGATTTCTGTCATGATCTGGCGCATCAGTGCCGAATCATCGACAGATAACACCCTGATTTTGCTCATCGTTAATCCTTACTTAGCGCATACA  >  NC_000913/1968279‑1968526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑