Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,971,962 G→A R244C (CGC→TGC)  tar ← methyl‑accepting chemotaxis protein II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,971,9620GA100.0% 127.5 / NA 38R244C (CGC→TGC) tarmethyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

CGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATA  >  NC_000913/1971843‑1972087
                                                                                                                       |                                                                                                                             
cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         >  1:309352/1‑126 (MQ=255)
cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         <  2:309352/126‑1 (MQ=255)
cgcAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            >  2:314866/1‑139 (MQ=255)
                ccGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTaccac                                                                                            >  1:210185/1‑139 (MQ=255)
                      tAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGa                                                                                      <  2:535082/139‑1 (MQ=255)
                                   aaCCTTCGCGGACATGAGAGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgtg                                                                         >  1:276765/1‑139 (MQ=255)
                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGt                                                                                                                   <  1:560418/91‑1 (MQ=255)
                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGt                                                                                                                   >  2:560418/1‑91 (MQ=255)
                                         cgcgGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCaat                                                                   >  2:417089/1‑139 (MQ=255)
                                                  gAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGc                                                          <  1:578766/139‑1 (MQ=255)
                                                    gTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCa                                                        <  1:417089/139‑1 (MQ=255)
                                                     agACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       <  2:276765/138‑1 (MQ=255)
                                                          gtgtCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         <  1:63082/68‑1 (MQ=255)
                                                          gtgtCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTc                                                                                                                         >  2:63082/1‑68 (MQ=255)
                                                             tcagtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTaa                                               >  1:362041/1‑139 (MQ=255)
                                                               agtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            >  2:197842/1‑76 (MQ=255)
                                                               agtcaAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTa                                                                                                            <  1:197842/76‑1 (MQ=255)
                                                                     agagCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       >  1:287194/1‑123 (MQ=255)
                                                                     agagCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAg                                                       <  2:287194/123‑1 (MQ=255)
                                                                         cGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCa                                                                                                                        >  2:207784/1‑54 (MQ=255)
                                                                         cGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCa                                                                                                                        <  1:207784/54‑1 (MQ=255)
                                                                         cGTTGCATATGAGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGc                                   <  1:390194/139‑1 (MQ=255)
                                                                             gCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAAt                               >  1:166346/1‑139 (MQ=255)
                                                                              cATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGa                                 >  2:50854/1‑136 (MQ=255)
                                                                              cATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGa                                 <  1:50854/136‑1 (MQ=255)
                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAg                                              >  2:502269/1‑115 (MQ=255)
                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAg                                              <  1:502269/115‑1 (MQ=255)
                                                                                      aaaCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCa                      >  2:509170/1‑139 (MQ=255)
                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGc                             >  1:196025/1‑130 (MQ=255)
                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGc                             <  2:196025/130‑1 (MQ=255)
                                                                                        aCGCTCTGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACg                    >  1:509684/1‑139 (MQ=255)
                                                                                           ctctGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgt                                                                          >  2:292616/1‑82 (MQ=255)
                                                                                           ctctGCGCCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAAtgt                                                                          <  1:292616/82‑1 (MQ=255)
                                                                                                cgcCAGGTCGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACcagc            <  2:527507/139‑1 (MQ=255)
                                                                                                        cGCCCATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCaa    >  2:435839/1‑139 (MQ=255)
                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCa     >  2:298631/1‑136 (MQ=255)
                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCa     <  1:298631/136‑1 (MQ=255)
                                                                                                          cccATTTCACTGCACCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATa  >  2:179989/1‑139 (MQ=255)
                                                                                                                       |                                                                                                                             
CGCAATTTCACGGGTACCGGCATAGATGGCATCTGAACCTTCGCGGACATGAGTGACGGTGTCAGTCAAAGAGCGTTGCATATGTGAAACGCTCTGCGCCAGGTCGCCCATTTCACTGCGCCCGTCAATGGTCAGGGTATTCGCCAGGTTACCACCGGCGATTTCGCGAATGTGAGCAATAATTTTTGCCAGCGGAGTAAGCAACATACGGCGAATGCCGTACCACGCCACCAGCAGAATCAATA  >  NC_000913/1971843‑1972087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑