Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,073,079	T→C	L514P (CTG→CCG)	flu →	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,073,079	0	T	C	100.0%	347.3 / NA	89	L514P (CTG→CCG)	flu	CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/38);  total (51/38)

CGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCG  >  NC_000913/2072945‑2073210                                                                                                                                       |                                                                                                                                    cGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCcgaat                                                                                                                                 >  2:262590/1‑139 (MQ=255)   aTACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCcgaatga                                                                                                                               >  1:398328/1‑139 (MQ=255)    tACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCcgaatgaa                                                                                                                              >  2:492759/1‑139 (MQ=255)         tGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCac                                                                                                                         >  2:201317/1‑139 (MQ=255)           aCCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgc                                                                                                                       >  2:64273/1‑139 (MQ=255)            ccATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgct                                                                                                                      <  2:449903/139‑1 (MQ=255)             cATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctg                                                                                                                     >  1:512705/1‑139 (MQ=255)              aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctga                                                                                                                    >  2:164715/1‑139 (MQ=255)              aTCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctga                                                                                                                    <  1:209822/139‑1 (MQ=255)                ccGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctgacg                                                                                                                  >  1:278040/1‑139 (MQ=255)                 cGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctgacgc                                                                                                                 >  1:504693/1‑139 (MQ=255)                   tGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgctgacgctg                                                                                                               <  2:474321/139‑1 (MQ=255)                     aaGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGaa                                                                                                             <  2:398328/139‑1 (MQ=255)                       ggCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGG‑AAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGa                                                                                                          >  1:62263/1‑139 (MQ=255)                           aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGTGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAgg                                                                                                                            <  1:387256/118‑1 (MQ=255)                           aTGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAgg                                                                                                                            >  2:387256/1‑118 (MQ=255)                                  ccTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTc                                                                                                >  1:450194/1‑139 (MQ=255)                                      cAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacca                                                                                            >  2:28800/1‑139 (MQ=255)                                             gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                     >  2:267102/1‑139 (MQ=255)                                             gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                     >  2:550095/1‑139 (MQ=255)                                             gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                     >  2:404752/1‑139 (MQ=255)                                             gCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGt                                                                                     >  2:211135/1‑139 (MQ=255)                                              ctctctCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTc                                                                                    >  1:132429/1‑139 (MQ=255)                                              ctctctCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTc                                                                                    <  2:163338/139‑1 (MQ=255)                                              ctctctCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTc                                                                                    >  1:68480/1‑139 (MQ=255)                                                    cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              <  1:262590/139‑1 (MQ=255)                                                    cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              <  1:564365/139‑1 (MQ=255)                                                    cACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              <  1:331936/139‑1 (MQ=255)                                                         gTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         <  1:64273/139‑1 (MQ=255)                                                             cGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacc                                                                                             <  1:106711/115‑1 (MQ=255)                                                             cGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTcacc                                                                                             >  2:106711/1‑115 (MQ=255)                                                             cGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGt                                                                     >  1:478964/1‑139 (MQ=255)                                                               gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                   <  2:478964/139‑1 (MQ=255)                                                               gCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTAc                                                                   >  1:359238/1‑139 (MQ=255)                                                                 aGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              >  2:129373/1‑126 (MQ=255)                                                                 aGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              <  1:129373/126‑1 (MQ=255)                                                                 aGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAg                                                                 <  2:504693/139‑1 (MQ=255)                                                                   cGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                               >  1:496445/1‑139 (MQ=255)                                                                                 gAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCgg                                                 <  2:551491/139‑1 (MQ=255)                                                                                      ggCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            <  1:203198/139‑1 (MQ=255)                                                                                             tcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGAc                                                                                                         <  2:35912/71‑1 (MQ=255)                                                                                             tcactGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGAc                                                                                                         >  1:35912/1‑71 (MQ=255)                                                                                                ctGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGaa                                  <  1:492759/139‑1 (MQ=255)                                                                                                 tGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAAc                                 >  1:483273/1‑139 (MQ=255)                                                                                                     aGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCa                                                                                   <  2:165160/85‑1 (MQ=255)                                                                                                     aGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCa                                                                                   >  1:165160/1‑85 (MQ=255)                                                                                                     aGCAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTg                             >  2:62077/1‑139 (MQ=255)                                                                                                       cAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTAcc                                                                                                   <  1:300645/67‑1 (MQ=255)                                                                                                       cAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTAcc                                                                                                   >  2:300645/1‑67 (MQ=255)                                                                                                       cAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            >  2:602237/1‑122 (MQ=255)                                                                                                       cAACACCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            <  1:602237/122‑1 (MQ=255)                                                                                                         acacCACACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAt                         >  2:533175/1‑139 (MQ=255)                                                                                                          caccacACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCAtt                        >  1:285707/1‑139 (MQ=255)                                                                                                            ccacACTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGa                      <  2:68480/139‑1 (MQ=255)                                                                                                             cacaCTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAg                                                                                                       <  2:261902/57‑1 (MQ=255)                                                                                                             cacaCTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAg                                                                                                       >  1:261902/1‑57 (MQ=255)                                                                                                             cacaCTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGAc                     >  2:7017/1‑139 (MQ=255)                                                                                                               cacTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgct                                                                                                                      >  2:301252/1‑40 (MQ=255)                                                                                                               cacTCACCCAGAAAGCCGTCAACCCGAATGAAGGCacgct                                                                                                                      <  1:301252/40‑1 (MQ=255)                                                                                                               cacTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         <  2:96715/85‑1 (MQ=255)                                                                                                               cacTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         >  1:96715/1‑85 (MQ=255)                                                                                                                 cTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa                                                                                        <  1:419716/68‑1 (MQ=255)                                                                                                                 cTCACCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGa                                                                                        >  2:419716/1‑68 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCacgctgacgctg                                                                                                               >  2:373013/1‑42 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCacgctgacgctg                                                                                                               <  1:373013/42‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTAGCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCATTGTGCTGAACGGTGCCATTGACCCCACGa              >  2:412671/1‑139 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         <  2:171415/80‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         >  1:171415/1‑80 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         >  2:540159/1‑80 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         <  1:540159/80‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                               >  2:178897/1‑90 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGcc                                                               <  1:178897/90‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            <  2:61557/109‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            >  1:61557/1‑109 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACg                                >  1:100869/1‑121 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACg                                <  2:100869/121‑1 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa              >  1:428954/1‑139 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa              >  2:862/1‑139 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa              >  1:597953/1‑139 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa              >  2:483704/1‑139 (MQ=255)                                                                                                                    aCCCAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGa              >  1:429434/1‑139 (MQ=255)                                                                                                                     cccAGAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGaa             <  1:267102/139‑1 (MQ=255)                                                                                                                         gAAAGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCTATTGACCCCACGAATGTc         <  1:340023/139‑1 (MQ=255)                                                                                                                          aaaGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              <  1:41563/69‑1 (MQ=255)                                                                                                                          aaaGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCt                                                                              >  2:41563/1‑69 (MQ=255)                                                                                                                          aaaGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGCCa        >  1:141890/1‑139 (MQ=255)                                                                                                                            aGCCGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCAct      <  1:7017/139‑1 (MQ=255)                                                                                                                              ccGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAgcg                                                                         <  1:372040/70‑1 (MQ=255)                                                                                                                              ccGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGAACCGTCACCACGGATGTCATTGCTCAgcg                                                                         >  2:372040/1‑70 (MQ=255)                                                                                                                               cGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            <  1:343007/98‑1 (MQ=255)                                                                                                                               cGTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGgcagca                                            >  2:343007/1‑98 (MQ=255)                                                                                                                                gTCAACCCGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCg  >  2:37806/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    CGATACCCTGACCATCCGTGAAGGCGATGCACTCCTGCAGGGAGGCTCTCTCACCGGTAACGGCAGCGTGGAAAAATCAGGAAGTGGCACACTCACTGTCAGCAACACCACACTCACCCAGAAAGCCGTCAACCTGAATGAAGGCACGCTGACGCTGAACGACAGTACCGTCACCACGGATGTCATTGCTCAGCGCGGTACAGCCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCG  >  NC_000913/2072945‑2073210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑