| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,184,452 | G→A | A22V (GCC→GTC) | thiD ← | hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,184,452 | 0 | G | A | 96.3% | 88.7 / ‑6.2 | 27 | A22V (GCC→GTC) | thiD | hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/12); minor base C (0/1); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGGCCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACAA > NC_000913/2184336‑2184568 | cgACAAAATCAGGCTCATTTCGATACACCGTCTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTCCGCAGCCATAAGCGTCAAGGGCCGAGAAGGTGTTAAGATCGCCCTGAAACCCCGCACCACCACt < 1:133771/139‑1 (MQ=255)cgACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGcac < 2:4469/132‑1 (MQ=255)cgACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGcac > 1:4469/1‑132 (MQ=255)cgACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt > 2:38402/1‑139 (MQ=255)cgACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt < 2:232388/139‑1 (MQ=255) aCAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCg > 2:18542/1‑139 (MQ=255) aaTGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCa < 1:18542/139‑1 (MQ=255) aCTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGtt > 2:138322/1‑139 (MQ=255) cGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCGCTCGGCTCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCa < 2:407927/139‑1 (MQ=255) cGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGAt < 2:253590/103‑1 (MQ=255) cGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGAt > 1:253590/1‑103 (MQ=255) gggTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt > 2:118405/1‑97 (MQ=255) gggTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt < 1:118405/97‑1 (MQ=255) tATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGcc < 1:38402/139‑1 (MQ=255) ccaGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGc > 2:253538/1‑139 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCagag > 2:253743/1‑139 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCagag < 2:524512/139‑1 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCagag > 2:53169/1‑139 (MQ=255) aGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCa > 1:385278/1‑139 (MQ=255) cgcCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCAt < 1:448297/128‑1 (MQ=255) cgcCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCAt > 2:448297/1‑128 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGcac > 2:147030/1‑42 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGcac < 1:147030/42‑1 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCAc > 1:69103/1‑99 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCAc < 2:69103/99‑1 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGa > 2:38854/1‑139 (MQ=255) tGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACaa < 2:385278/139‑1 (MQ=255) | CGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGGCCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACAA > NC_000913/2184336‑2184568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |