Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,916	A→G	Q181Q (CAA→CAG)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,916	0	A	G	100.0%	100.5 / NA	27	Q181Q (CAA→CAG)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/14);  total (13/14)

CCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCA  >  NC_000913/2484786‑2485050                                                                                                                                   |                                                                                                                                       ccaaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTcc                                                                                                                                <  1:465268/139‑1 (MQ=255)  caaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCa                                                                                                                               <  2:510491/139‑1 (MQ=255)                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCCAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCttt                                                                                                           >  2:144570/1‑139 (MQ=255)                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGca                                                                                                                         >  1:595428/1‑124 (MQ=255)                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGca                                                                                                                         <  2:595428/124‑1 (MQ=255)                                           ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAGATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAtt                                                                                      >  1:87934/1‑139 (MQ=255)                                           ttCACGACTCAATGCGACCACTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                                              <  2:241842/131‑1 (MQ=255)                                           ttCACGACTCAATGCGACCACTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                                              >  1:241842/1‑131 (MQ=255)                                                   tCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCg                                                                              >  2:72049/1‑139 (MQ=255)                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                                                  >  1:156387/1‑139 (MQ=255)                                                                  aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                                               <  1:144570/139‑1 (MQ=255)                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                                           <  2:222246/139‑1 (MQ=255)                                                                                       atTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta                                          <  1:331095/139‑1 (MQ=255)                                                                                         tGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc                                        >  1:441964/1‑139 (MQ=255)                                                                                         tGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc                                        >  1:192916/1‑139 (MQ=255)                                                                                          gCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca                                       >  1:178934/1‑139 (MQ=255)                                                                                                 tAGCAAATTACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAc                                <  1:72049/139‑1 (MQ=255)                                                                                                         tACCCCCCAGACGAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTCTATTGGTAGTAACATCATTACCAGCAGTa                        >  2:99700/1‑139 (MQ=255)                                                                                                                     gAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta                                          <  2:415646/109‑1 (MQ=255)                                                                                                                     gAGGTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta                                          >  1:415646/1‑109 (MQ=255)                                                                                                                        gTAATTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTAtt         >  2:30992/1‑139 (MQ=255)                                                                                                                           aTTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag                             >  2:254420/1‑116 (MQ=255)                                                                                                                           aTTCATCAGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcag                             <  1:254420/116‑1 (MQ=255)                                                                                                                             tcatcaGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcact    <  2:184109/139‑1 (MQ=255)                                                                                                                             tcatcaGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcact    <  2:441964/139‑1 (MQ=255)                                                                                                                             tcatcaGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcact    <  1:30992/139‑1 (MQ=255)                                                                                                                             tcatcaGTCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcact    <  2:87934/139‑1 (MQ=255)                                                                                                                               atcagtcaTTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca  <  2:178934/139‑1 (MQ=255)                                                                                                                               atcagtcaTTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca  <  2:377212/139‑1 (MQ=255)                                                                                                                               atcagtcaTTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca  >  1:377212/1‑139 (MQ=255)                                                                                                                               atcagtcaTTTACAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactca  <  1:99700/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCA  >  NC_000913/2484786‑2485050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑