Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 322,417 T→A V27E (GTG→GAG)  ykgF → ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913322,4170TA100.0% 110.6 / NA 32V27E (GTG→GAG) ykgFferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (15/17);  total (15/17)

TCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATCATGTTCTGAGTAATCTCGACGCTTATCTGTAC  >  NC_000913/322287‑322550
                                                                                                                                  |                                                                                                                                     
tCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGAAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAAcg                                                                                                                               <  1:229996/139‑1 (MQ=255)
       gATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCgcagca                                                                                                                        <  1:244142/139‑1 (MQ=255)
       gATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCgcagca                                                                                                                        <  1:523462/139‑1 (MQ=255)
                       gtgtTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAAt                                                                                                        >  1:514648/1‑139 (MQ=255)
                               gaGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGc                                                                                                                                    <  1:229207/103‑1 (MQ=255)
                               gaGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGc                                                                                                                                    >  2:229207/1‑103 (MQ=255)
                                     cTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGt                                                                                          >  2:532091/1‑139 (MQ=255)
                                                    tGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGC‑AAAAATGGTCGATGAATTGGGGCa                                                                           >  1:385493/1‑138 (MQ=255)
                                                    tGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGC‑AAAAATGGTCGATGAATTGGGGCa                                                                           <  2:385493/138‑1 (MQ=255)
                                                     gTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCAc                                                                          >  2:73436/1‑139 (MQ=255)
                                                      tCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         >  2:299983/1‑139 (MQ=255)
                                                      tCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         <  2:447575/139‑1 (MQ=255)
                                                       cGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTg                                                                        >  1:318020/1‑139 (MQ=255)
                                                                    tAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         <  1:150680/125‑1 (MQ=255)
                                                                    tAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         >  2:150680/1‑125 (MQ=255)
                                                                                tAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         <  2:435646/113‑1 (MQ=255)
                                                                                tAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGgccgcc                                               <  1:532091/139‑1 (MQ=255)
                                                                                tAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGgccgcc                                               <  2:514648/139‑1 (MQ=255)
                                                                                tAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGACAAATCGGCAAAAAATGGTCGATGAATTGGGGCACt                                                                         >  1:435646/1‑113 (MQ=255)
                                                                                        gCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTg                                                                                >  1:214216/1‑98 (MQ=255)
                                                                                        gCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTg                                                                                <  2:214216/98‑1 (MQ=255)
                                                                                        gCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       <  1:73436/139‑1 (MQ=255)
                                                                                        gCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       <  1:314240/139‑1 (MQ=255)
                                                                                             cGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAATATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATc                                  <  2:318020/139‑1 (MQ=255)
                                                                                                          aaGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       <  1:46877/121‑1 (MQ=255)
                                                                                                          aaGATCCGATCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       >  2:46877/1‑121 (MQ=255)
                                                                                                                  aTCATGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATCATGTTCTGAGTAATCTCGACg             >  1:485730/1‑139 (MQ=255)
                                                                                                                     aTGCGCAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATCATGTTCTGAGTAATCTCGACGCtt          <  1:321034/139‑1 (MQ=255)
                                                                                                                       gcgcAAAGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATCATGTTCTGAGTAATCTCGACGCTTAt        >  1:3191/1‑139 (MQ=255)
                                                                                                                             aGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       >  1:580357/1‑102 (MQ=255)
                                                                                                                             aGCGGAGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACg                                       <  2:580357/102‑1 (MQ=255)
                                                                                                                             aGCGGAGGCAAACGAGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGTGTGGCGCGATCGGGCCGCCCAGAAACGTGATCGTGTTCTGAGTAATCTCGACGCTTATCTGTaa  >  1:605000/1‑138 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TCAAAGTGATGCATATTGCTGAAGTGTTGATGAGCCGCTGAGGATATAAAGATGTCGATCAAAACCAGTAATACAGATTTTAAGACACGCATCCGTCAGCAAATTGAAGATCCGATCATGCGCAAAGCGGTGGCAAACGCGCAGCAGCGTATTGGGGCAAATCGGCAAAAAATGGTCGATGAATTGGGGCACTGGGAGGAGTGGCGCGATCGGGCCGCCCAGATACGTGATCATGTTCTGAGTAATCTCGACGCTTATCTGTAC  >  NC_000913/322287‑322550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑