Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,691,927	G→A	A1206V (GCG→GTG)	purL ←	phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,691,927	0	G	A	100.0%	74.1 / NA	23	A1206V (GCG→GTG)	purL	phosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

AGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAT  >  NC_000913/2691797‑2692056                                                                                                                                   |                                                                                                                                  aGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                           >  1:18921/1‑139 (MQ=255)            ccGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcgcg                                                                                                                <  2:161910/139‑1 (MQ=255)             cGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCTCGCACTTCCACgcgc                                                                                                               <  1:350710/139‑1 (MQ=255)                 aTACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCtt                                                                                                           <  2:18921/139‑1 (MQ=255)                      gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCa                                                                                                      <  1:497540/139‑1 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                  >  2:154652/1‑126 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                  <  1:154652/126‑1 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGccc                                                                                                             <  2:459868/131‑1 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGccc                                                                                                             >  1:459868/1‑131 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCAt                                                                                                     >  1:191226/1‑139 (MQ=255)                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCAt                                                                                                     >  1:67333/1‑139 (MQ=255)                            tGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATgagag                                                                                                >  2:41881/1‑139 (MQ=255)                                   ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCt                                                                                                            <  1:49719/120‑1 (MQ=255)                                   ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCt                                                                                                            >  2:49719/1‑120 (MQ=255)                                    tCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAAt                                                                                        >  1:163924/1‑139 (MQ=255)                                        gTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTcc                                                                                                                     <  2:217072/106‑1 (MQ=255)                                        gTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTcc                                                                                                                     >  1:217072/1‑106 (MQ=255)                                                              aCTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCt                                                              >  1:200382/1‑139 (MQ=255)                                                                                           tGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                 >  2:492625/1‑139 (MQ=255)                                                                                                            ttgccAGTGCCGCCAGATGCGCCACAGCCCGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAAcg                <  1:33182/136‑1 (MQ=255)                                                                                                                      cgccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAg      <  2:249010/139‑1 (MQ=255)                                                                                                                          aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt  <  1:492625/139‑1 (MQ=255)                                                                                                                          aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt  <  1:225738/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  AGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAT  >  NC_000913/2691797‑2692056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑