Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,899	T→C	intergenic (‑39/+160)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,899	0	T	C	100.0%	108.1 / NA	30	intergenic (‑39/+160)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

GCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTT  >  NC_000913/2817764‑2818034                                                                                                                                        |                                                                                                                                        gCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGccact                                                                                                                                      <  1:190043/139‑1 (MQ=255)     aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAg                                                                                                                                  >  1:279643/1‑139 (MQ=255)     aaGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAg                                                                                                                                  >  2:290380/1‑139 (MQ=255)          tACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCata                                                                                                                             <  2:149981/139‑1 (MQ=255)           aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  2:298940/139‑1 (MQ=255)           aCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:321090/139‑1 (MQ=255)            cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            >  2:290916/1‑138 (MQ=255)            cGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:290916/138‑1 (MQ=255)                   tatGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAag                                                                                                                    >  2:538278/1‑139 (MQ=255)                          cATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGt                                                                                                             <  2:490810/139‑1 (MQ=255)                           aTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTg                                                                                                            >  1:455058/1‑139 (MQ=255)                                 gaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCg                                                                                                      >  2:317644/1‑139 (MQ=255)                                  aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCa                                                                                                                               <  1:287223/113‑1 (MQ=255)                                  aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCa                                                                                                                               >  2:287223/1‑113 (MQ=255)                                     aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTATAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                                  <  2:455058/139‑1 (MQ=255)                                     aTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggag                                                                                                  <  1:538278/139‑1 (MQ=255)                                                                        aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGtt                                                               <  1:290380/139‑1 (MQ=255)                                                                              tCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTaaa                                                         >  2:398845/1‑139 (MQ=255)                                                                                  gCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCaa                                                     <  1:398845/139‑1 (MQ=255)                                                                                   cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                            <  1:309934/99‑1 (MQ=255)                                                                                   cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTAc                                                                                            >  2:309934/1‑99 (MQ=255)                                                                                   cTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAAc                                                    >  2:393698/1‑139 (MQ=255)                                                                                      aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            <  1:299702/64‑1 (MQ=255)                                                                                      aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCatat                                                                                                                            >  2:299702/1‑64 (MQ=255)                                                                                        cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  2:279643/139‑1 (MQ=255)                                                                                        cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  1:317644/139‑1 (MQ=255)                                                                                                gCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTa                                       >  2:110173/1‑139 (MQ=255)                                                                                                                       gctgATAATGATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                <  1:503510/139‑1 (MQ=255)                                                                                                                                gATACCGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGAt       <  2:393753/139‑1 (MQ=255)                                                                                                                                     cGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTat                                               <  1:133460/94‑1 (MQ=255)                                                                                                                                     cGCCACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt  >  1:601593/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTT  >  NC_000913/2817764‑2818034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑