Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,913,461 T→C E64G (GAG→GGG)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,4610TC100.0% 133.0 / NA 35E64G (GAG→GGG) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/16);  total (19/16)

CGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAT  >  NC_000913/2913327‑2913591
                                                                                                                                      |                                                                                                                                  
cGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCccac                                                                                                                                <  2:187519/139‑1 (MQ=255)
cGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCccac                                                                                                                                <  2:385591/139‑1 (MQ=255)
        aGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCaa                                                                                                                        >  1:218698/1‑139 (MQ=255)
                   cgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGc                                                                                                                <  2:609923/136‑1 (MQ=255)
                   cgacTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGc                                                                                                                >  1:609923/1‑136 (MQ=255)
                                ctctCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTgg                                                                                                >  1:350904/1‑139 (MQ=255)
                                   tcACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCCCGCCACAATAACAGACTGGCAt                                                                                             <  1:115792/139‑1 (MQ=255)
                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATACCAGACTGGCATCCGGATGccc                                                                                   >  2:360377/1‑139 (MQ=255)
                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGAc                                                                                                   <  1:278133/123‑1 (MQ=255)
                                             caTCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGAc                                                                                                   >  2:278133/1‑123 (MQ=255)
                                                   cGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  2:569970/139‑1 (MQ=255)
                                                   cGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  1:360377/139‑1 (MQ=255)
                                                     cTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCt                                                                           >  2:370679/1‑139 (MQ=255)
                                                           aCGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCa                                                                     <  2:257686/139‑1 (MQ=255)
                                                               tGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATGCccac                                                                                                                                <  2:576276/76‑1 (MQ=255)
                                                                  cATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATa                                                              >  2:499205/1‑139 (MQ=255)
                                                                      cGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGcc                                                          <  1:370679/139‑1 (MQ=255)
                                                                                     cagcGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAg                                           <  2:218698/139‑1 (MQ=255)
                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             <  1:115155/102‑1 (MQ=255)
                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGt                                                                             >  2:115155/1‑102 (MQ=255)
                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGtt                                                                        >  2:60581/1‑107 (MQ=255)
                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGtt                                                                        <  1:60581/107‑1 (MQ=255)
                                                                                        cgccgcCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCAc                                        >  2:87909/1‑139 (MQ=255)
                                                                                          ccgcccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTc                                      >  2:252842/1‑139 (MQ=255)
                                                                                             cccgcAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTcaca                                   >  2:26728/1‑139 (MQ=255)
                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGttt                                                   <  1:60065/96‑1 (MQ=255)
                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGttt                                                   >  2:60065/1‑96 (MQ=255)
                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACt                                       <  2:454316/108‑1 (MQ=255)
                                                                                                                        aTGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACt                                       >  1:454316/1‑108 (MQ=255)
                                                                                                                         tGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACtt       >  2:103467/1‑139 (MQ=255)
                                                                                                                          gTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTg      <  2:350904/139‑1 (MQ=255)
                                                                                                                          gTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAACACTCACACGACTTCTGGCTGGTAATACCCAGACTTg      >  2:606551/1‑139 (MQ=255)
                                                                                                                            cGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCg    >  1:91349/1‑139 (MQ=255)
                                                                                                                              agagGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt  >  1:91624/1‑139 (MQ=255)
                                                                                                                              agagGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAt  >  1:302792/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                  
CGTGAATAAGGTTAACGACCGACTTACCGACGCTCTCACGCAGCACATCTTCGCTGACTACGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTCAATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTCTGGCTGGTAATACCCAGACTTGCGAT  >  NC_000913/2913327‑2913591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: