Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,055,285	T→C	T54A (ACG→GCG)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,055,285	0	T	C	100.0%	113.2 / NA	30	T54A (ACG→GCG)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

CATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGTCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGT  >  NC_000913/3055162‑3055404                                                                                                                            |                                                                                                                        catcGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAg                                                                                                          <  2:334401/139‑1 (MQ=255)    cGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCgg                                                                                                       <  1:327395/139‑1 (MQ=255)       cATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAg                                                                                                    >  1:509199/1‑139 (MQ=255)             gCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGa                                                                                              <  1:64188/139‑1 (MQ=255)              cAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAg                                                                                             <  1:605845/139‑1 (MQ=255)                          aaaaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTa                                                                                 <  1:561272/139‑1 (MQ=255)                          aaaaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCC‑TCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTAc                                                                                <  1:158954/139‑1 (MQ=255)                            aaaGGAAGCCGTCATCCTGCAGGACATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTAcc                                                                               <  2:553234/139‑1 (MQ=255)                             aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCg                                                                              <  2:301553/139‑1 (MQ=255)                               ggAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGcc                                                                            >  1:299576/1‑139 (MQ=255)                                 aaGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCcac                                                                          >  2:238738/1‑139 (MQ=255)                                       tCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGACGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATatca                                                                    <  2:399373/139‑1 (MQ=255)                                               cAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTg                                                            >  2:15051/1‑139 (MQ=255)                                                  ggCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGAtt                                                         >  1:490716/1‑139 (MQ=255)                                                        gCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCa                                                   <  1:478271/139‑1 (MQ=255)                                                            gtggCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCa                                               >  2:121274/1‑139 (MQ=255)                                                                 agagTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCa                                          >  2:531710/1‑139 (MQ=255)                                                                        aTTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTgggg                                   <  2:299576/139‑1 (MQ=255)                                                                               gCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCAT‑CCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGAccc                           >  2:547/1‑139 (MQ=255)                                                                                cAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGAccc                           <  1:531710/139‑1 (MQ=255)                                                                                  gTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGt                         >  2:577626/1‑139 (MQ=255)                                                                                   tGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTc                        >  1:159374/1‑139 (MQ=255)                                                                                        gtgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttg                   >  1:114258/1‑139 (MQ=255)                                                                                         tgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttgt                  <  2:490716/139‑1 (MQ=255)                                                                                         tgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttgt                  <  1:15051/139‑1 (MQ=255)                                                                                               cTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTc            >  2:32635/1‑139 (MQ=255)                                                                                               cTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTc            >  1:155141/1‑139 (MQ=255)                                                                                                   tGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGAt        >  1:78617/1‑139 (MQ=255)                                                                                                    gACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATa       <  1:238738/139‑1 (MQ=255)                                                                                                         aaaGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGt  >  1:193498/1‑139 (MQ=255)                                                                                                                            |                                                                                                                        CATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGTCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGT  >  NC_000913/3055162‑3055404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑