Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	133.6 / NA	38	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/23);  total (15/23)

GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTC  >  NC_000913/3366825‑3367083                                                                                                                                 |                                                                                                                                   gTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCT‑GTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                         <  2:503272/139‑1 (MQ=255)  ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                         <  2:358334/139‑1 (MQ=255)       cTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGc                                                                                                                    <  1:149897/139‑1 (MQ=255)                      gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                     <  2:256066/139‑1 (MQ=255)                        tCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCg                                                                                                   >  2:572669/1‑139 (MQ=255)                          cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                 >  2:290149/1‑139 (MQ=255)                             cTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                              <  2:561537/139‑1 (MQ=255)                              ttttAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                             <  1:431927/139‑1 (MQ=255)                                                   aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAgg                                                                        <  1:517579/139‑1 (MQ=255)                                                                    ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCa                                                       <  1:247084/139‑1 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                       >  1:599845/1‑120 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                                                                       <  2:599845/120‑1 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTg                                                         >  2:12706/1‑134 (MQ=255)                                                                       cAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTg                                                         <  1:12706/134‑1 (MQ=255)                                                                         ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                  <  2:22616/139‑1 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                >  2:583214/1‑139 (MQ=255)                                                                           cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                >  1:257723/1‑139 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                     >  2:180957/1‑83 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                     <  1:180957/83‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTgg                                                               >  2:227721/1‑121 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTgg                                                               <  1:227721/121‑1 (MQ=255)                                                                              tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTc                                             <  2:449383/139‑1 (MQ=255)                                                                                        cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                   <  1:290149/139‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGc                                                     >  1:496131/1‑113 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGc                                                     <  2:496131/113‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                  <  1:144628/116‑1 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                  >  2:144628/1‑116 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                           >  2:400606/1‑139 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                           >  2:117643/1‑139 (MQ=255)                                                                                                ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                           <  1:89837/139‑1 (MQ=255)                                                                                                        gAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAgttgtt                   <  2:329797/139‑1 (MQ=255)                                                                                                           cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                            <  2:319540/63‑1 (MQ=255)                                                                                                           cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                            >  1:319540/1‑63 (MQ=255)                                                                                                             aTGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt              <  2:257723/139‑1 (MQ=255)                                                                                                              tGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTc             >  2:474269/1‑139 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt              >  1:602366/1‑128 (MQ=255)                                                                                                                        cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACtt              <  2:602366/128‑1 (MQ=255)                                                                                                                         tGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc  <  1:583214/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                   GTTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTC  >  NC_000913/3366825‑3367083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑