Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,880,017	C→T	G35S (GGT→AGT)	gyrB ←	DNA gyrase, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,880,017	0	C	T	100.0%	162.0 / NA	48	G35S (GGT→AGT)	gyrB	DNA gyrase, subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/22);  total (26/22)

CGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879883‑3880150                                                                                                                                       |                                                                                                                                      cGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTg                                                                                                                                   >  1:404649/1‑139 (MQ=255)     aTGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGt                                                                                                                               >  2:152843/1‑139 (MQ=255)       gCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCa                                                                                                                             >  2:25882/1‑139 (MQ=255)                gTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCg                                                                                                                    <  1:64063/139‑1 (MQ=255)                   aTCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:591229/139‑1 (MQ=255)                        gTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGAtata                                                                                                            >  1:73728/1‑139 (MQ=255)                              agacagagTTATCGGCGTGAATGGTGATGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATAcc                                                                                                      >  2:275245/1‑139 (MQ=255)                                acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCg                                                                                                    >  2:400342/1‑139 (MQ=255)                                acagagTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCg                                                                                                    >  2:353568/1‑139 (MQ=255)                                  agagTTATCGGCGTGAATGGTGATGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCggg                                                                                                  <  1:275245/139‑1 (MQ=255)                                         tCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:73675/1‑117 (MQ=255)                                         tCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:73675/117‑1 (MQ=255)                                             cGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCAc                                                                                       <  1:152843/139‑1 (MQ=255)                                                       gACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  1:353568/139‑1 (MQ=255)                                                           aTAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCtt                                                                         >  2:457203/1‑139 (MQ=255)                                                                          tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:384510/84‑1 (MQ=255)                                                                          tGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:384510/1‑84 (MQ=255)                                                                            aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCTCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  1:385413/1‑139 (MQ=255)                                                                            aCCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTg                                                        >  2:111075/1‑139 (MQ=255)                                                                               cgcgAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:81018/138‑1 (MQ=255)                                                                               cgcgAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:81018/1‑138 (MQ=255)                                                                                      gcTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCAt                                              <  1:25882/139‑1 (MQ=255)                                                                                                             caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGcc                                                                                                                  <  2:24338/48‑1 (MQ=255)                                                                                                             caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGcc                                                                                                                  >  1:24338/1‑48 (MQ=255)                                                                                                             caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTGCGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:355914/1‑108 (MQ=255)                                                                                                             caccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:355914/108‑1 (MQ=255)                                                                                                              accTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  2:136137/84‑1 (MQ=255)                                                                                                              accTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             >  1:136137/1‑84 (MQ=255)                                                                                                               ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 >  1:242732/1‑47 (MQ=255)                                                                                                               ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCg                                                                                                                 <  2:242732/47‑1 (MQ=255)                                                                                                               ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             >  1:26842/1‑83 (MQ=255)                                                                                                               ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGc                                                                             <  2:26842/83‑1 (MQ=255)                                                                                                               ccTCGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCg                     <  1:111075/139‑1 (MQ=255)                                                                                                                  cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      >  1:44062/1‑103 (MQ=255)                                                                                                                  cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTgga                                                      <  2:44062/103‑1 (MQ=255)                                                                                                                  cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  1:485307/1‑139 (MQ=255)                                                                                                                  cGAATACCATGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTc                  >  2:213348/1‑139 (MQ=255)                                                                                                                          aTGTGGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATAc          <  1:213348/139‑1 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTc                                                                       <  2:337133/77‑1 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTc                                                                       >  1:337133/1‑77 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggag                                                     >  1:265991/1‑95 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTggag                                                     <  2:265991/95‑1 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                   >  1:251234/1‑129 (MQ=255)                                                                                                                           tgtgGTGCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCt                   <  2:251234/129‑1 (MQ=255)                                                                                                                               gtgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTggg     >  2:609389/1‑139 (MQ=255)                                                                                                                                tgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGgcgc                                                                                               <  2:514802/48‑1 (MQ=255)                                                                                                                                tgCAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGgcgc                                                                                               >  1:514802/1‑48 (MQ=255)                                                                                                                                  cAGACTGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTa  <  2:385413/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CGGAATGCCGCGCCCGTCATCCTGTACAGAGACAGAGTTATCGGCGTGAATGGTGACGATAATTTCTTTACAGTGACCCGCGAGCGCTTCGTCGATAGCGTTATCTACCACCTCGAATACCATGTGGTGCAGACCGGTGCCGTCATCCGTGTCGCCGATATACATACCCGGGCGCTTACGCACCGCATCCAGCCCTTTCAGGACTTTGATACTGGAGGAGTCATAAGAATTCGACATCAACGTTTCTCGCTCATTTATACTTGGGTTA  >  NC_000913/3879883‑3880150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑