Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,188,572	C→T	R1075C (CGT→TGT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,188,572	0	C	T	100.0%	76.9 / NA	23	R1075C (CGT→TGT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

TACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGAC  >  NC_000913/4188460‑4188706                                                                                                                 |                                                                                                                                       tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                              <  1:72761/139‑1 (MQ=255) tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                              <  2:48549/139‑1 (MQ=255)         tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTaa                                                                                                      >  2:493035/1‑139 (MQ=255)             cGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgac                                                                                                  >  1:422582/1‑139 (MQ=255)                cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTg                                                                                                                   <  1:18246/119‑1 (MQ=255)                cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTg                                                                                                                   >  2:18246/1‑119 (MQ=255)                cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGCTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGGGGTAAAGATCTCTGTCGGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                               >  1:468092/1‑139 (MQ=255)                         cATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCa                                                                                      <  1:456023/139‑1 (MQ=255)                             aCGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                                                                  >  2:290953/1‑139 (MQ=255)                                                                  tggtggTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACAGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGc                                             <  2:599425/139‑1 (MQ=255)                                                                     tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAt                                          >  1:136207/1‑139 (MQ=255)                                                                                 ccGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                              <  1:290953/139‑1 (MQ=255)                                                                                 ccGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                              <  2:422582/139‑1 (MQ=255)                                                                                  cGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                     <  2:316732/131‑1 (MQ=255)                                                                                  cGCAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                     >  1:316732/1‑131 (MQ=255)                                                                                    cAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                           <  2:136207/139‑1 (MQ=255)                                                                                    cAGAACGTACCGCAGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                           <  1:493035/139‑1 (MQ=255)                                                                                                 aGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                              <  1:4015/43‑1 (MQ=255)                                                                                                 aGGTGGTAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCt                                                                                                              >  2:4015/1‑43 (MQ=255)                                                                                                     ggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                         <  2:88966/60‑1 (MQ=255)                                                                                                     ggtAAAGATCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATc                                                                                         >  1:88966/1‑60 (MQ=255)                                                                                                      gtAAAGATCTGTGTCCGGCACTGAAAATCGTTGCTGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc         >  2:17992/1‑139 (MQ=255)                                                                                                             tCTGTGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGac  >  1:46369/1‑139 (MQ=255)                                                                                                                 |                                                                                                                                       TACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGAC  >  NC_000913/4188460‑4188706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑