Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,604	C→T	R227C (CGC→TGC)	zraS →	sensory histidine kinase in two‑component regulatory system with ZraR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,604	0	C	T	100.0%	37.2 / NA	12	R227C (CGC→TGC)	zraS	sensory histidine kinase in two‑component regulatory system with ZraR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

ATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCA  >  NC_000913/4202472‑4202702                                                                                                                                     |                                                                                                   aTCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCACAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTcgc                                                                                              >  1:48004/1‑139 (MQ=255)                  tGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt                                                                                                 <  1:15308/119‑1 (MQ=255)                  tGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCt                                                                                                 >  2:15308/1‑119 (MQ=255)                          ttGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGatgaaatgaa                                                                     <  2:256951/139‑1 (MQ=255)                               aCCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCa                                                                >  2:438076/1‑139 (MQ=255)                                cccAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCaa                                                               >  2:375376/1‑139 (MQ=255)                                    gagTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAagag                                                           >  2:88820/1‑139 (MQ=255)                                          ggAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCtg                                                     <  1:88820/139‑1 (MQ=255)                                                                  ccTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCa                             >  1:603692/1‑139 (MQ=255)                                                                             tGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCa                  >  2:67855/1‑139 (MQ=255)                                                                                          gCTGGCAAGCGTATTGTCATTCTTCTGTTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGACAGGCGTTGCCCACGAAATCCGTAAc     <  1:438076/139‑1 (MQ=255)                                                                                             ggCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGTGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCa  <  2:603692/139‑1 (MQ=255)                                                                                                                                     |                                                                                                   ATCGCCGTTGATGCCAGTGATCTGGTTGCAACCCAGAGTGGGGAAAAGCGCAATACCCTGATTATCCTCTTCGCCCTGGCGACGGTCTTGCTGGCAAGCGTATTGTCATTCTTCTGGTATCGCCGCTATCTGCGCTCGCGCCAGCTTCTACAAGATGAAATGAAGCGCAAAGAGAAGCTGGTGGCGCTGGGGCATCTTGCGGCAGGCGTTGCCCACGAAATCCGTAACCCA  >  NC_000913/4202472‑4202702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑