| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,281,472 | A→G | E498G (GAG→GGG) | yjcE → | putative cation/proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,281,472 | 0 | A | G | 100.0% | 81.6 / NA | 22 | E498G (GAG→GGG) | yjcE | putative cation/proton antiporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/13); total (9/13) | |||||||||||
AGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAA > NC_000913/4281343‑4281602 | agCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGa > 2:238169/1‑139 (MQ=255) aTACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCt < 1:421217/139‑1 (MQ=255) tAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGaa > 2:576995/1‑139 (MQ=255) aaCCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAAc < 2:491746/139‑1 (MQ=255) aGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgc > 2:297338/1‑139 (MQ=255) gTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcg < 1:204839/139‑1 (MQ=255) tctcGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcg < 1:111530/139‑1 (MQ=255) gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc < 1:57155/136‑1 (MQ=255) gcgTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc > 2:57155/1‑136 (MQ=255) cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcg > 1:340954/1‑118 (MQ=255) cgtcgtCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcg < 2:340954/118‑1 (MQ=255) gtcgtcGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTg > 1:242355/1‑96 (MQ=255) gtcgtcGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTg < 2:242355/96‑1 (MQ=255) aCGTAACGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTa < 1:297338/139‑1 (MQ=255) aaCGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt > 1:471746/1‑62 (MQ=255) aaCGACGTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCAtt < 2:471746/62‑1 (MQ=255) acgacgTTGAAAGTTCCGTGCAGGAATGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTAAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCa < 2:219024/139‑1 (MQ=255) gTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACg > 1:67251/1‑117 (MQ=255) gTTGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACg < 2:67251/117‑1 (MQ=255) ttGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc < 1:6631/91‑1 (MQ=255) ttGAAAGTTCCGTGCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTgcgcgc > 2:6631/1‑91 (MQ=255) gCAGGAAGGGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGaa < 1:576995/139‑1 (MQ=255) | AGCGTCTGGCGGCGGATACCGAAGAGAATATCGATAACCAGCTGCTTACGGAGGTCAGTTCTCGCGTCATTGGTAACCTGCGTCGTCGCGCCGATGGACGTAACGACGTTGAAAGTTCCGTGCAGGAAGAGAACCTTGAGCGTCGCTTCCGTCTGGCGGCATTGCGTTCTGAACGTGCTGAACTTTACCACCTGCGCGCCACACGGGAGATCAGCAACGAAACGCTGCAAAAATTACTGCACGATCTCGATTTGCTTGAA > NC_000913/4281343‑4281602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |