Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	764,978	G→A	E267K (GAA→AAA)	sucD →	succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	764,978	0	G	A	100.0%	58.8 / NA	18	E267K (GAA→AAA)	sucD	succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

TACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAG  >  NC_000913/764876‑765091                                                                                                       |                                                                                                                  tACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCgg                                                                               <  2:148615/139‑1 (MQ=255)        cTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCgg                                                                        >  1:368715/1‑139 (MQ=255)             gtgACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAg                                                                   <  2:112890/139‑1 (MQ=255)               gACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCa                                                                 <  2:127495/139‑1 (MQ=255)                     gcCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGcc                                                                                     <  2:317579/113‑1 (MQ=255)                     gcCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGcc                                                                                     >  1:317579/1‑113 (MQ=255)                                                   tGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGa                             >  1:71975/1‑139 (MQ=255)                                                              ccGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGaaataaat                                          <  1:193265/115‑1 (MQ=255)                                                              ccGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGaaataaat                                          >  2:193265/1‑115 (MQ=255)                                                               cGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTcgc                 <  2:71975/139‑1 (MQ=255)                                                                  tGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCg                                                                                          <  2:284419/63‑1 (MQ=255)                                                                  tGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCg                                                                                          >  1:284419/1‑63 (MQ=255)                                                                   gggAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCt             >  1:22990/1‑139 (MQ=255)                                                                    ggAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCtt            >  2:341599/1‑139 (MQ=255)                                                                      aaaGGGACTGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTcc          <  2:22990/139‑1 (MQ=255)                                                                              tGCGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAg  >  1:346502/1‑139 (MQ=255)                                                                                cGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTcc          >  1:608016/1‑129 (MQ=255)                                                                                cGGATGAGAAATTCGCTGCTCTGAAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTcc          <  2:608016/129‑1 (MQ=255)                                                                                                       |                                                                                                                  TACATCGCTGGTGTGACTGCGCCGAAAGGCAAACGTATGGGCCACGCGGGTGCCATCATTGCCGGTGGGAAAGGGACTGCGGATGAGAAATTCGCTGCTCTGGAAGCCGCAGGCGTGAAAACCGTTCGCAGCCTGGCGGATATCGGTGAAGCACTGAAAACTGTTCTGAAATAAATATCTGTAATAAGAAATAGCCCTCGCCGCTTCCCTCTACAG  >  NC_000913/764876‑765091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑