Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,673 G→A G223R (GGA→AGA)  creB → response regulator in two‑component regulatory system with CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,6730GA100.0% 82.2 / NA 25G223R (GGA→AGA) creBresponse regulator in two‑component regulatory system with CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/12);  total (13/12)

CAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAA  >  NC_000913/4636538‑4636807
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cAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGat                                                                                                                                     <  1:203533/139‑1 (MQ=255)
         gATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGAGGCATGAGATATAGCCTGa                                                                                                                            <  2:558970/139‑1 (MQ=255)
                   gggAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTa                                                                                                                  >  2:233328/1‑139 (MQ=255)
                        gATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCg                                                                                                             >  2:131605/1‑139 (MQ=255)
                                        aCGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGgttg                                                                                             >  2:500077/1‑139 (MQ=255)
                                               cACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                    <  2:46574/125‑1 (MQ=255)
                                               cACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                    >  1:46574/1‑125 (MQ=255)
                                               cACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTgg                                                                                      >  1:551722/1‑139 (MQ=255)
                                                                     aCGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                    >  1:426234/1‑103 (MQ=255)
                                                                     aCGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCAt                                                                                                    <  2:426234/103‑1 (MQ=255)
                                                                      cGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTggtggcggtg                                                               <  1:201306/139‑1 (MQ=255)
                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAgg                                                                                                                          >  1:277625/1‑75 (MQ=255)
                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAgg                                                                                                                          <  2:277625/75‑1 (MQ=255)
                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                          <  1:500077/139‑1 (MQ=255)
                                                                           cGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGc                                                          <  1:233328/139‑1 (MQ=255)
                                                                                cAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGt                                                     <  2:551722/139‑1 (MQ=255)
                                                                                                        tCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAgggg                                                                                                                        >  1:388264/1‑48 (MQ=255)
                                                                                                        tCTTTCACCGATTAATACTCATCGCGGCATGAGATATAGCCTGAgggg                                                                                                                        <  2:388264/48‑1 (MQ=255)
                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAc                    >  2:536218/1‑138 (MQ=255)
                                                                                                                  aTTAATACTCATCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAc                    <  1:536218/138‑1 (MQ=255)
                                                                                                                            aTCGCGGCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGa         >  1:476635/1‑139 (MQ=255)
                                                                                                                                 ggCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGc    >  1:116455/1‑139 (MQ=255)
                                                                                                                                 ggCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGc    >  1:200255/1‑139 (MQ=255)
                                                                                                                                 ggCATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGc    <  1:131605/139‑1 (MQ=255)
                                                                                                                                   cATGAGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCaa  >  2:451994/1‑139 (MQ=255)
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CAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAA  >  NC_000913/4636538‑4636807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: