Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,037,464 A→G D239G (GAC→GGC)  hyaF → hydrogenase‑1 protein nickel incorporation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,037,4640AG100.0% 98.4 / NA 29D239G (GAC→GGC) hyaFhydrogenase‑1 protein nickel incorporation factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/12);  total (17/12)

AGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGCTGCA  >  NC_000913/1037338‑1037597
                                                                                                                              |                                                                                                                                     
aGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAgg                                                                                                                           >  2:456962/1‑139 (MQ=255)
  gCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGcc                                                                                                                         <  2:452163/139‑1 (MQ=255)
      aTCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGtt                                                                                                                     >  1:313712/1‑139 (MQ=255)
                             tgggggCCGGGAAATATTCAGATTCGTACCTTTGGCTAGGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGACCGTTACTGGAAAGTTATGAAATCTGcc                                                                                              <  1:220008/136‑1 (MQ=255)
                              gtgGGCCGGGAACTATTTAGTTTCGTACCATTGGCTATGGCGAGAGCTATATCAACCCCACGGGGTTACTCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGccc                                                                                             <  1:456962/139‑1 (MQ=255)
                                  gCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATa                                                                                         >  2:76087/1‑139 (MQ=255)
                                   ccGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATAc                                                                                        <  2:205479/139‑1 (MQ=255)
                                      ggAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGcc                                                                                              <  2:448165/130‑1 (MQ=255)
                                      ggAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGcc                                                                                              >  1:448165/1‑130 (MQ=255)
                                      ggAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCgg                                                                                     >  2:306512/1‑139 (MQ=255)
                                                                 tATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAACGTTATGAAATCTGCCCACTACCGGAAGTGGTGCTGGCAGCGCCAGAAGAtt                                                          >  1:344775/1‑139 (MQ=255)
                                                                    ggCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTgg                                                       >  2:520419/1‑139 (MQ=255)
                                                                    ggCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTgg                                                       >  1:268192/1‑139 (MQ=255)
                                                                        agagCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGa                                                                                                               >  1:129591/1‑79 (MQ=255)
                                                                        agagCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGa                                                                                                               <  2:129591/79‑1 (MQ=255)
                                                                        agagCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGccc                                                                                             <  1:227868/97‑1 (MQ=255)
                                                                        agagCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGccc                                                                                             >  2:227868/1‑97 (MQ=255)
                                                                               tatCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGaa                                                                                   <  1:3435/100‑1 (MQ=255)
                                                                               tatCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGaa                                                                                   >  2:3435/1‑100 (MQ=255)
                                                                                    aCGCCACGGGGTTACCCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAAGACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCg                                       >  1:408013/1‑139 (MQ=255)
                                                                                          cGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAg                                 <  1:379769/139‑1 (MQ=255)
                                                                                                  cGCCATGTCTGGCATTTACGCTGTACGGGTACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTAt                         >  1:267243/1‑139 (MQ=255)
                                                                                                  cGCCATGTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTAt                         >  2:495332/1‑139 (MQ=255)
                                                                                                        gTCTGGCATTTACGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGt                   >  2:553815/1‑139 (MQ=255)
                                                                                                                   aCGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGc                                >  2:357967/1‑115 (MQ=255)
                                                                                                                   aCGCTGTACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGc                                <  1:357967/115‑1 (MQ=255)
                                                                                                                         tACGGGTACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGCTGCa  <  2:267243/139‑1 (MQ=255)
                                                                                                                         tACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGtggctggc            >  2:291297/1‑129 (MQ=255)
                                                                                                                         tACGGGCACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGtggctggc            <  1:291297/129‑1 (MQ=255)
                                                                                                                              |                                                                                                                                     
AGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACCTTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGACTCTGCGCAGCGGCTTAGCGAGGTATGTCAGTGGCTGGCGGAAGCTGCA  >  NC_000913/1037338‑1037597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: